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Molecular Plant 2023年第8期16卷 1269-1282页

作者： Maherun Nisa Thomas eekhout Clara Bergis Jose-Antonio Pedroza-Garcia Xiaoning He Christelle Mazubert Ilse Vercauteren Toon Cools Rim Brik-Chaouche Jeannine Drouin-Wahbi Layla Chmaiss David Latrasse Catherine Bergounioux Klaas Vandepoele Moussa Benhamed Lieven De Veylder Cécile Raynaud Universite Paris-Saclay CNRSINRAEUniversite EvryInstitute of Plant Sciences Paris-Saclay(IPS2)91190 Gif-sur-YvetteFrance Universite de Paris Cite CNRSINRAEInstitute of Plant Sciences Paris-Saclay(IPS2)91190 Gif-sur-YvetteFrance Department of Plant Biotechnology and Bioinformatics Ghent University9052 GhentBelgium Center for Plant Systems Biology VIB9052 GhentBelgium Present address:Plant Molecular and Cellular Biology Laboratory Salk Institute for Biological StudiesLa JollaCA 92037USA

Survival of living organisms is fully dependent on their maintenance of genome integrity,being permanently threatened by replication stress in proliferating *** the plant DNA damage response(DDR)regulator SOG1 has been demonstrated to cope with replication defects,accumulating evidence points to other pathways functioning independent of ***,we report the roles of the Arabidopsis e2fA and ef2B transcription factors,two well-characterized regulators of DNA replication,in plant response to replication *** a combination of reverse genetics and chromatin immunoprecipitation approaches,we show that e2fA and e2fB share many target genes with SOG1,providing evidence for their involvement in the *** of double-and triple-mutant combinations revealed that e2fB,rather than e2fA,plays the most prominent role in sustaining plant growth in the presence of replication defects,either operating antagonistically or synergistically with ***,SOG1 aids in overcoming the replication defects of e2fA/e2fB-deficient ***,our data reveal a complex transcriptional network controlling the replication stress response in which e2fs and SOG1 act as key regulatory factors.

关键词： replication stress e2f SOG1 cell cycle Arabidopsis

e2f transcription factors and their complementary roles during DNA damage responses

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Molecular Plant 2023年第9期16卷 1373-1375页

作者： Paula Casati Centro de estudios fotosinteticos y Bioquimicos(CefOBl) Universidad Nacional de RosarioSuipacha 5312000 RosarioArgentina

e2f transcription factors(Tf)comprise a family of proteins that regulate the expression of genes required for S-phase progression,among *** genome encodes six e2f Tfs(e2fA–f),which are classified as typical(e2fA–C)and atypical(e2fD–f)depending on their ability to interact with two DP interaction proteins(DPa and DPb)(Sablowski and Gutierrez,2022).While e2fA–C need to heterodimerize with either DPa or DPb to function,e2fD–f act independently of DP ***,the retinoblastoma-related1(RBR1)protein also binds and inhibits the typical e2f/DP Tf complexes when bound to the promoter of genes,repressing their expression in the absence of a signal to enter a new cell *** general,e2fA and e2fB are considered transcriptional activators,while e2fC is generally considered a repressor of cell proliferation(Sablowski and Gutierrez,2022).In addition,it is well documented that e2fB at least,and probably e2fA,can also stimulate the G2/M transition.

关键词： damage e2f complementary

MicroRNA-let-7a promotes e2f-mediated cell proliferation and NfKB activation in vitro

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Cellular & Molecular Immunology 2014年第1期11卷 79-93页

作者： Cristen B Chafin Nicole L Regna David L Caudell Christopher M Reilly Department of Biomedical Sciences & Pathobiology Virginia-Maryland Regional College of Veterinary Medicine Virginia Polytechnic Institute and StateUniversity Blacksburg VA USA Department of Pathology/Comparative Medicine Wake Forest University Primate Center Wake Forest School ofMedicine Winston-Salem NC USA edward Via College of Osteopathic Medicine Blacksburg VA USA

epigenetic factors, including altered microRNA （miRNA） expression, may contribute to aberrant immune cell function in systemic lupus erythematosus （SLe）. MiRNA-let-7a （let-7a） has been shown to directly alter cell cycle progression and proinflammatory cytokine production. Due to the crucial role of let-7a in cell division and inflammation, we investigated let-7a-mediated proliferation and NfKB translocation in J774A. 1 macrophages and MeS 13 mesangial cells in vitro. In immune-stimulated cells transfected with let-7a, cell proliferation was significantly increased over time. There was a significant increase in the number of immune-stimulated cells in S and G2 phases. Immune-stimulated cells overexpressing let-7a had increased nuclear translocation of NfKB. Bioinformatical analysis revealed that the e2f family, critical regulators of the G1-S transition, has potential binding sites for let-7a in their mRNA transcripts. Let-7a overexpression significantly increased the expression of the cell cycle activator e2f2 and increased retinoblastoma protein （Rb） phosphorylation in immune-stimulated cells. The cell cycle inhibitor e2f5 was significantly decreased in let-7a-transfected cells that were immune-stimulated. Bioinformatical analysis revealed e2f2 and NfKB are transcription factors predicted to regulate the let-7a promoter. We analyzed transcriptional regulation of let-7a by real-time RT-PCR using chromatin immunoprecipitation with e2f2 and NfKB antibodies. There was an increase in e2f2 and NfKB binding in DNA enriched for the let-7a promoter in immune-stimulated cells. Silencing e2f2 or NfKB significantly decreased let-7a expression and IL-6 production in immune-stimulated cells. Taken together, our results suggest that overexpression of let-7a may contribute to hyperplasia and the proinflammatory response in SLe.

关键词： cell proliferation e2f microRNA-let-7a Nf~:B systemic lupus erythematosus

Different Patterns of Cyclin D1/CDK4-e2f-1/4 Pathways in Human embryo Lung fibroblasts Treated by Benzo[a]pyrene at Different Doses

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Biomedical and environmental Sciences 2008年第1期21卷 30-36页

作者： MeNG Ye BING-CI LIU XIANG-LIN SHI BAO-RONG YOU HONG-JU DU XIAO-WeI JIA fU-HAI SHeN National Institute of Occupational Health and Poison Control Chinese Center for Disease Control and Prevention 29 Nanwei Road Beijing 100050 China Shanghai Institute for Nutritional Sciences Chinese Academy of Sciences 249 Taiyuan Road Shanghai 200031 China

Objective To investigate the roles of the cyclin D1/CDK4 and e2f-1/4 pathways and compare their work patterns in cell cycle changes induced by different doses of B[a]e Methods Human embryo lung fibroblasts （HeLfs） were treated with 2 μmol/L or 100 μmol/L B[a]P which were provided with some characteristics of transformed cells （T-HeLfs）. Cyclin D l, CDK4 and e2f-1/4 expressions were determined by Western blotting. flow cytometry was used to detect the distribution of cell cycle. Results After B[a]P treatment, the proportion of the first gap （G 1） phase cells decreased. CDK4 and e2f-4 expression did not change significantly. In 2 μmol/L treated cells, a marked overexpression of cyclin D1 and e2f-1 was observed. However, in T-HeLfs overexpression was limited to cyclin D1 only, and no overexpression of e2f-1 was observed. The decreases of G1 phase in response to B[a]P treatment were blocked in antisense cyclin D1 and antisense CDK4 transfected HeLfs （A-D1 and A-K4） and T-HeLfs （T-A-D1 and T-A-K4）. After 2 μmol/L B[a]P treatment, overexpression of e2f-1 was attenuated in A-D1, and e2f-4 expression was decreased significantly in A-K4. In T-A-D1 and T-A-K4, e2f-4 expression was increased significantly, compared with T-HeLfs. The e2f-1 expression remained unchanged in T-A-D1 and T-A-K4. Condusions Cyclin DI/CDK4-e2f-1/4 pathways work in different patterns in response to low dose and high dose B[a]P treatment. In HeLfs treated with 2 μmol/L B[a]P, cyclin D1 positively regulates the e2f-1 expression while CDK4 negatively regulates the e2f-4 expression; however, in HeLfs treated with 100 μmol/L B[a]P, both cyclin D1 and CDK4 negatively regulate the e2f-4 expression.

关键词： Benzo[a]pyrene Cyclin D1 CDK4 e2f Cell cycle

Vitamin C Inhibits Benzo[a]pyrene-Induced Cell Cycle Changes Partly via Cyclin D1/e2f Pathway in Human embryo Lung fibroblasts

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Biomedical and environmental Sciences 2006年第3期19卷 239-244页

作者： AI GAO BING-CI LIU XIANG-LIN SHI CHUAN-SHU HUANG XIAO-WeI JIA BAO-RONG YOU MeNG Ye fU-HAI SHeN HONG-JU DU National Institute of Occupation Health and Poison Control Chinese Center for Disease Control and Prevention Beijing 100050 China National Institute for Occupational Safety and Health 1095 Willowdale RoadMorgantown WV26506 USA Nelson Institute of environmental Medicine New York University School of Medicine 57 Old Forge Road Tuxedo NY 10987

Objective To study the molecular mechanism of the inhibitory effects of vitamin C on benzo[a]pyrene （B[a]P）-induced changes of cell cycle in human embryo lung fibroblast （HeLf） cells. Methods The stable transfectants, HeLf transfected with antisense cyclin D1 and antisense CDK4, were established. Cells were cultured and pretreated with vitamin C before stimulation with B[a]P for 24 h. The expression levels of cyclin DI, CDK4, e2fI, and e2f4 were determined by Western blot. flow cytometric analysis was employed to detect the distributions of cell cycle. Results B[a]P significantly elevated the expression levels of cyclin D 1, e2f1, and e2f4 in HeLf cells. Vitamin C decreased the expression levels of cyclin D 1, e2f1, and e2f4 in B [a]P-stimulated HeLf cells. Dose-dependent relationships were not found between the different concentrations of vitamin C （10, 100, 500, 1000, and 5000 lamol/L） and the expression levels of cyclin D 1, e2f1, and e2f4 in HeLf cells. The expression levels of cyclin D1, e2fI, and e2f4 in B[a]P-treated transfectants were lower than those in B[a]P-treated HeLf cells. The expression levels of cyclin DI and e2f4 treated with vitamin C and antisense cyclin D1 were decreased compared with those treated with antisense cyclin DI alone. The effects of vitamin C combined with antisense CDK4 on the expression levels of cyclin DI and e2fI/e2f4 were similar to those of antisense CDK4 alone. B[a]P progressed HeLf cells from GI to S phase. Both vitamin C and antisense cyclin DI suppressed the changes of cell cycle progressed by B[a]P. However, antisense CDK4 did not attenuate the above changes. Vitamin C combined with antisense CDK4 markedly suppressed B[a]P-induced changes of cell cycle as compared with antisense CDK4. But the inhibitory effects of vitamin C combined with antisense cyclin DI on B[a]P-induced changes of cell cycle were similar to those of vitamin C alone or antisense cyclin DI alone. Conclusions B[a]P progressed HeLf cells from G1 to S phase

关键词： B[a]P Ascorbic acid Cyclin D1 e2f Cell cycle Antisense

e2f transcription factors and digestive system malignancies:How much do we know?

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World Journal of Gastroenterology 2014年第29期20卷 10212-10216页

作者： Konstantinos evangelou Sophia Havaki Athanassios Kotsinas Laboratory Histology-embryology Medical SchoolNational and Kapodistrian University of Athens

The e2f proteins comprise a family of 8 members that function as transcription factors. They are key targets of the retinoblastoma protein (RB) and were initially divided into groups of activators and repressors. Accumulating data suggest that there is no specific role for each individual e2f member. Instead, each e2f can exert a variety of cellular effects, some of which represent opposing ones. for instance, specific e2fs can activate transcription and repression, promote or hamper cell proliferation, augment or inhibit apoptosis, all being dependent on the cellular context. This complexity reflects the importance that these transcription factors have on a cell’s fate. Thus, delineating the specific role for each e2f member in specific malignancies, although not easy, is a challenging and continuously pursued task, especially in view of potential e2f targeted therapies. Therefore, several reviews are continuously trying to evaluate available data on e2f status in various malignancies. Such reviews have attempted to reach a consensus, often in the simplistic form of oncogenes or tumor suppressor genes for the e2fs. However they frequently miss spatial and temporal alterations of these factors during tumor development, which should also be considered in conjunction with the status of the regulatory networks that these factors participate in. In the current ‘‘Letter to the editor’’, we comment on the flaws, misinterpretations and omissions in one such review article published recently in the World Journal of Gastroenterology regarding the role of e2fs in digestive system malignancies.

关键词： e2f Hepatocellular carcinoma Pancreatic ductal adenocarcinoma Gastrointestinal tract Digestive system p53 p73 Cancer Apoptosis Proliferation

SeNeX gene promotes cell proliferation by activating RB/e2f pathway in diffuse large B-cell lymphoma cells

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BIOCeLL 2021年第4期45卷 933-942页

作者： JIYU WANG YING PAN YANG WAN ZHIXIANG WANYAN ZHITAO WANG QIANSHAN TAO ZHIMIN ZHAI Department of Hematology The Second Hospital of Anhui Medical UniversityHefei230601China

The present study aimed to clarify the role of SeNeX in malignant cell proliferation in diffuse large B-cell lymphoma(DLBCL).22 DLBCL patients(6 newly diagnosed cases,7 cases at complete remission,and 9 relapsed cases)were included in the *** results indicated that both SeNeX gene and protein were significantly increased in peripheral blood mononuclear cells(PBMCs)and tumor cells of relapsed DLBCL patients,accompanied by overexpression of p21,p16,and phosphorylated retinoblastoma(Rb).Silencing the SeNeX gene in a DLBCL cell line caused a significant decrease in cell proliferation and inhibited cell cycle progression in the G1 *** Rb and e2f1 were also decreased,and activation of the Rb/e2f1 pathway was obviously *** conclude,the SeNeX gene promotes proliferation in PBMC and tumor cells of DLBCL patients by activating the Rb/e2f1 pathway,in a manner suggesting that increased SeNeX expression affects the relapse of DLBCL and may serve as an important target for DLBCL therapy.

关键词： SeNeX DLBCL Cell proliferation Rb e2f

Non-Canonical functions of the e2f/DP Pathway with emphasis in Plants

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Phyton-International Journal of experimental Botany 2021年第2期90卷 307-330页

作者： Víctor A.Sánchez-Camargo Samantha Romero-Rodríguez Jorge M.Vázquez-Ramos facultad de Química Departamento de BioquímicaUniversidad Nacional Autónoma de MéxicoAvenida Universidad y CopilcoCiudad de México04510México Plant Development and(epi)Genetics Group Swammerdam Institute for Life SciencesUniversiteit van AmsterdamAmsterdam1098XHNetherlands

The e2f/DP pathway is a widely conserved regulatory mechanism in pluricellular *** family of e2f and DP transcription factors was originally described having a role in the transition from the G1 to the S phase of the cell ***,the discovery of hundreds of possible gene targets and their involvement in many other biochemical processes,soon showed that they participated in cell development and differentiation,chromatin remodeling,DNA repair and *** e2f/DP transcription factors can act as either activators or repressors of transcription depending on their association to other regulatory proteins,particularly the retinoblastoma protein,or even depending to their protein structure that can define their *** plants the e2f/DP pathway also regulates endoreduplication,a process present along the life cycle,from organ elongation to root differentiation and *** transcription factors also help plant cells to respond to environmental disturbances such as those caused by different types of radiation,or by *** review focuses on the“so called”non-canonical functions of the e2f/DP family proteins in animal and plant cells,that are in fact essential activities that connect regulatory circuits among multiple metabolic pathways by means of their atypical functions.

关键词： e2f DP transcription factors atypical functions plant cells

基于e2f通路构建前列腺癌无复发生存的预测模型及关键基因生物学功能的研究

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基于E2F通路构建前列腺癌无复发生存的预测模型及关键基因生物学...

作者：陈磊安徽医科大学

学位级别：硕士

背景:生化复发(biochemical recurrence,BCR)显著影响前列腺癌患者的预后,本研究旨在深入探索与前列腺癌生化复发相关的信号通路及关键基因,揭示前列腺癌生化复发的分子机制。方法:从癌症基因组图谱(The Cancer Genome Atlas,TCGA)和基... 详细信息

背景:生化复发(biochemical recurrence,BCR)显著影响前列腺癌患者的预后,本研究旨在深入探索与前列腺癌生化复发相关的信号通路及关键基因,揭示前列腺癌生化复发的分子机制。方法:从癌症基因组图谱(The Cancer Genome Atlas,TCGA)和基因表达综合(Gene expression Omnibus,GeO)数据库中选取5个前列腺癌队列,在发生和未发生生化复发的前列腺癌患者间进行基因集变异分析(gene set variation analysis,GSVA)。将5个前列腺癌队列的GSVA分析结果取交集,进一步筛选得到前列腺癌生化复发相关的信号通路,并提取该信号通路相对应的基因列表。通过单因素Cox回归分析筛选前列腺癌生化复发相关的基因,使用LASSO-Cox回归分析进一步筛选生化复发相关的基因,根据筛选的基因构建并验证前列腺癌无复发生存(recurrence free survival,RfS)相关基因模型,绘制并验证无生化复发相关的列线图模型。通过MTT和平板克隆形成实验,验证筛选的前列腺癌生化复发相关基因对前列腺癌细胞增殖等生物学行为的影响,进一步验证上述生物信息学分析结果。结果:与未发生生化复发的前列腺癌患者相比,发生生化复发的前列腺癌患者中e2f通路被激活,因此,提取e2f通路中的基因名称,在纪念斯隆凯特琳癌症中心(Memorial Sloan Kettering Cancer Center,MSKCC)前列腺癌队列中,基于筛选出的前列腺癌生化复发相关基因,建立了一个基于e2f通路相关基因的前列腺癌无复发生存相关的预测模型。该基因模型是基于四个鉴定出的e2f通路相关基因而建立的,即CDKN2C,CDKN3,RACGAP1和RRM2四个基因。在MSKCC队列中,根据CDKN2C,CDKN3,RACGAP1和RRM2四个基因的表达水平和其相应的回归系数,计算出每个前列腺癌患者的风险评分,低风险评分的前列腺癌患者比高风险评分的患者无复发生存时间长。受试者工作特征曲线(Receiver operating characteristic,ROC)分析显示,e2f通路相关基因模型在预测前列腺癌无生化复发方面具有较高的准确性,并在TCGA数据库的前列腺癌队列中验证了上述模型的预测价值。亚组分析结果显示:与低Gleason评分、T分期较早的前列腺癌患者相比,高Gleason评分、T分期较晚的前列腺癌患者具有更高的风险评分。在MSKCC队列中,基于e2f通路相关基因模型构建的列线图风险模型能够较准确的预测前列腺癌患者发生生化复发的可能性,且在TCGA前列腺癌队列中进一步验证了该列线图具有较高的预测价值。体外细胞功能实验发现:敲低CDKN2C和RACGAP1基因的表达显著抑制前列腺癌细胞的增殖等生物学行为,证实了上述生物信息学分析的结果。结论:e2f通路与前列腺癌的生化复发过程相关,筛选出的四个e2f通路相关基因对前列腺癌细胞的生物学行为具有重要影响,且基于四个e2f通路相关基因建立的e2f通路相关基因模型及列线图模型在预测前列腺癌生化复发方面具有较高的准确性。

关键词：前列腺癌生化复发 e2f 基因模型 TCGA GeO