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Development and utilization of new sequenced characterized amplified region markers specific for e genome of Thinopyrum

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Frontiers in Biology 2013年第4期8卷 451-459页

作者： Wenping GONG Ling RAN Guangrong LI Jianping ZHOU Cheng LIU Zujun YANG School of Life Science and Technology University of Electronic Science and Technology of China Chengdu 610054 China

Species containing e genome of Thinopyrum offered potential to increase the genetic variability and desirable characters for wheat improvement. However, e genome specific marker was rare. The objective of the present report was to develop and identify sequenced characterized amplified region （SCAR） markers that can be used in detecting e chromosome in wheat background for breeding purpose. Total 280 random amplified polymorphic DNA （RAPD） primers were amplified for seeking of e genome specific fragments by using the genomic DNA of Thinopyrum elongatum and wheat controls as templates. As a result, six RAPD fragments specific for e genome were found and cloned, and then were converted to SCAR markers. The usability of these markers was validated using a number of e- genome-containing species and wheat as controls. These markers were subsequently located on e chromosomes using specific PCR and fluorescence in situ hybridization （FISH）. SCAR markers developed in this research could be used in molecular marker assisted selection of wheat breeding with Thinopyrum ehromatin introgressions.

关键词： Thinopyrum Trititrigia e genome SCAR markers FISH

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Genetic Relationships Among Five Basic genomes St, e, A, B and D in Triticeae Revealed by Genomic Southern and in situ Hybridization

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Journal of Integrative Plant Biology 2007年第7期49卷 1080-1086页

作者： Zhao Liu Dayong Li Xueyong Zhang Key Laboratory of Crop Germplasm and Biotechnology Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China.

The St and e are two important basic genomes in the perennial tribe Triticeae （Poaceae）. They exist in many perennial species and are very closely related to the A, B and D genomes of bread wheat （Triticum aestivum L.）. Genomic Southern hybridization and genomic in situ hybridization （GISH） were used to analyze the genomic relationships between the two genomes （St and e） and the three basic genomes （A, B and D） of T. aestivum. The semi-quantitative analysis of the Southern hybridization suggested that both St and e genomes are most closely related to the D genome, then the A genome, and relatively distant to the B genome. GISH analysis using St and e genomic DNA as probes further confirmed the conclusion. St and e are the two basic genomes of Thinopyrum ponticum （StSte^ee^be^x） and Th. intermedium （Ste^ee^b）, two perennial species successfully used in wheat improvement. Therefore, this paper provides a possible answer as to why most of the spontaneous wheat-Thinopyrum translocations and substitutions usually happen in the D genome, some in the A genome and rarely in the B genome. This would develop further use of alien species for wheat improvement, especially those containing St or e in their genome components.

关键词： e genome fluorescent in situ hybridization genomic in situ hybridization perennial Triticeae St genome wheat,

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