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duplex pcr Detection of Pseudomonas savastanoi *** and Curtobacterium flaccumfaciens pv,flaccumfaciens in Soybean

Plant Diseases and Pests 2016年第1期7卷 27-31页

作者： Liu Yan Yang Wanfeng Liu Xiang Shao Peize Chen Yunqing Zhao Wenjun Lianyungang Agriculture Science Institute/Jiangsu Collaborative Innovation Center for Modern Corp Production Lianyungang 222006China Lianyungang Entry-Exit Inspection and Quarantine Bureau Lianyungang 222042China Institute of Plant Quarantine Chinese Academy of Inspection and QuarantineBeijing 100029China

The paper aimed to establish a duplex pcr method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp in GenBank, the primers PSPF1/PSPR2 were designed. The duplex pcr assay was dereloped using the combined primers PSPF1/PSPR2 and CflF1/CffR2, which were specific primers for Cff. The reaction conditions were optimized and specificity md sensitivity of the duplex pcr were tested. The expected DNA fragment was specifically amplified from the genomic DNA of Psp and Cff. Specificity was conirmed in the artificially inoculated soybean samples imparted. Thus, the duplex pcr developed in this study could be used for the simultaneous detection of Psp md Cff from imported soybean.

关键词： Pseudomonas savastanoi pv. phaseolicola (Psp) Curtobacteriumflaccumfacierts pv. flaccumfaciens (Gff) duplex pcr Detection method

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Characterization of a duplex pcr assay for the detection of enterotoxigenic strains of Staphylococcus aureus

Characterization of a duplex PCR assay for the detection of ...

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中国食品科学技术学会第五届年会暨第四届东西方食品业高层论坛

作者： Junni Tang~(1,2) Xianming Shi Chunlei Shi~1 Huanchun Chen~2 (1Department of Food Science and Technology and Bor Luh Food Safety Center,Shanghai Jiao Tong University,Shanghai 201101 China)(2National Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070 China)

Sequence analysis demonstrated that all reported primers based on the thermonuclease gene (nuc)used in the detection of the food-borne pathogen,Staphylococcus aureus,were derived from the nuclease gene,instead of the thermonuclease *** this study,two pairs of primers targeting the sequences of the nuc gene and 16S rRNA gene were designed in a duplex pcr assay for the detection of ***,particularly those of the enterotoxigenic *** optimization of the amplification conditions by uniform design,two specific products were amplified in the pcr detection system:a 223-bp nuc fragment and a 565-bp 16S rDNA *** tests demonstrated that this duplex-pcr method has high specificity for only *** detection limit of the assay was 9.35 pg/μl using *** genomic *** sensitivity for direct detection of milk samples sparked with S. aureus was 104 to 105 CFU/ml,and the sensitivity was 1 CFU/ml as an initial concentration when an enrichment process was used.A pcr-based detection assay for enterotoxin gene was performed in parallel with the detection of the nuc-encoded TNase activity in several *** strains,and a good correlation between TNase production and enterotoxigenicity was demonstrated in all the strains tested.

关键词： duplex pcr uniform design Staphylococcus aureus rapid detection

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Development of a novel duplex real-time pcr for cell-free DNA quantification and its Clinical application of cell death assessment for trauma patients of the Wenchuan earthquake in China

Development of a novel duplex real-time PCR for cell-free DN...

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中华医学会第八次全国检验医学学术会议暨中华医学会检验分...

作者：潘世扬陈丹束永前南京医科大学第一附属医院

Backgrou nd It has long been known that cell-free DNA concentrations increase in the circulating blood of patients with various ***,the most important problem is that there was not an accurate and stable method.

关键词： plasma DNA quantification duplex pcr real-time pcr earthquake trauma

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