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Expression And Clinical Significance of Glucose Regulated Proteins GRP78 And GRP94 in Human Colon Cancer

Chinese Journal of Cancer Research 2010年第1期22卷 42-48页

作者： Ming-hua Liu Meng-chunWang Na Gao Yan Li Wei-guo Jiang Department of Gastroenterology Department of pathology Shengjing Hospital China Medical University Shenyang 110004 China Endoscopy Center Liaoning Tumour Hospital Shenyang 110000 China

Objective： To investigate the expression of glucose regulated proteins GRP78 and GRP94 in human colon cancer. Methods： Tissues of resected primary colon cancer, colon adenoma and normal tissue were investigated. Protein expression was detected with immunohistochemical staining, mRNA expression levels of GRP78 and GRP94 were determined by semiquantitative reverse transcriptase polymerase chain reaction （RT-PCR） after mRNA extraction. Results： The expression of GRP94 and GRP78 was significantly higher in colon cancer when compared to those in colon adenoma and normal tissue （P〈0.01）. GRP94 mRNA and protein expression was found to be in close relationship with the grade of differentiation, dukes stages, lymph node involvement and remote metastasis in colon cancer （P〈0.01）, but no relationship with gender and age （P〉0.05）. GRP78 mRNA and protein expression increased with cancer progression along the normal tissue-adenoma-cancer sequence, but showed no association with grade of differentiation, dukes stages, lymph node involvement, remote metastasis, gender and age （P〉0.05）. The mRNA expression of GRP78 and GRP94 was consistent with the proteins （P〈0.01）, but there is no correlation between overexpression of GRP78 and GRP94 （P〉0.05）, and the patients with both strong GRP78 and GRP94 protein expression did not show advanced tumor stages （P〉0.05）. Conclusion： Overexpression of GRP78 and GRP94 was found in colon cancer. Overexpression of GRP94 was closely related to cellular differentiation, dukes stages, invasion and metastasis.

关键词： Colon cancer GRP78 GRP94 Colon adenoma dukes stages Metastasis

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Prognostic values of tumor endothelial markers in patients with colorectal cancer

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World Journal of Gastroenterology 2005年第9期11卷 1283-1286页

作者： KA Rmali MCA Puntis WG Jiang Metastasis and Angiogenesis Research Group University Department of Surgery

AIM: Tumor endothelial markers (TEMs) are a newly discovered family of endothelial markers associated with tumor specificangiogenesis. This study sought to examine the levels of expression (qualitatively and quantitatively)for TEMs in human colon ***: Human colorectal cancer tissues (n = 48)and normal background tissues (n = 31) were obtained after surgery. RNA was extracted from frozen sections for gene amplification. The expression of TEMs (TEM-1to TEM-8) was assessed using RT-PCR and their transcript levels were determined using real-time-quantitative PCR(Q-RT-PCR).RESULTS: TEM-1 (P = 0.01), TEM-7 (P = 0.04), TEM-7R(P= 0.03), TEM-8 (P = 0.001) significantly raised in colon cancer tissues compared with the levels detected in normal background tissues. The expressions of TEM-2 and TEM-6were found to be not significantly different between tumor tissues and normal tissues (P＞0.05). Patients who had cancer penetrating into and through the muscularis propria of the bowel wall and developed nodal involvement(dukes C) exhibited significantly higher levels of TEM -8compared to patients who were node negative (P＜0.05).TEM-7 and TEM-7R showed high level of transcripts in dukes C, but they were not statistically ***: The level of the expression of TEM-1,TEM-7, TEM-7R and TEM-8 (but not TEM-2 and TEM-6)were associated with both nodal involvement and disease progression, and may therefore, have a prognostic value in colorectal cancer.

关键词： Colon cancer Angiogenesis Tumor endothelial markers dukes stages

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