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Inhibitory effect of berberine on morphine tolerance and hyperalgesia in mice

中医杂志:英文版 2023年第5期43卷 915-924页

作者： HAN Shuai MAO Hua JIN Tingting YAN Rubing WANG Ziyi ZHANG Jie SHI Jianwen LIANG Yongxin Department of Anesthesiology Women’s and Children’s Hospital Affiliated to Qingdao UniversityQingdao 266034China Yangzhou University Yangzhou 225009China Qingdao University Qingdao 266071China Department of Anesthesiology the Affiliated Hospital of Qingdao UniversityQingdao 266075China

OBJECTIVE: To evaluate the effect of berberine on morphine analgesia, tolerance, and hyperalgesia. METHODS: Morphine-induced acute tolerance model: mice received intraperitoneal berberine at doses of 2.5, 5.0, and 10 mg/kg;30 min later, subcutaneous morphine 10 mg/kg was injected every hour for nine continuous h. Morphine 10 mg/kg alone was administered at 24 and 48 h. Morphine-induced chronic tolerance model: mice received intraperitoneal berberine 2.5, 5.0, and 10 mg/kg;30 min later, 10 mg/kg morphine was injected subcutaneously for eight consecutive days. On the ninth day, morphine 10 mg/kg was given alone. Morphineinduced established tolerance model: mice were injected subcutaneously with morphine 10 mg/kg once a day for eight consecutive days. Berberine 2.5 mg/kg was administered on day one, four, and seven and morphine 10 mg/kg alone on day nine. The baseline latency(T0) and post-treatment latency(T1) were determined by the hot plate test, and the maximum possible analgesic effect (MPAE) was calculated. Nitric oxide synthase(NOS) activity and nitric oxide(NO) content in the spinal cord were measured by spectrophotometer. Verification of berberine analgesic effect by blocking N-methyl-Daspartate(NMDA) receptor: HT-22 and HEK-293 cells transfected with NMDA plasmid were randomly divided into five groups: control group, NMDA group, berberine low-dose, medium-dose, and high-dose groups(5, 10, 20 μmol/L, respectively). Except for the control group, cells were treated with NMDA(HT-22 cells: 20 mmol/L;HEK-293 cells: 50 μmol/L). After 24 h, cell viability was detected by cell counting kit-8. The molecular mechanism between berberine and the NMDA receptor was studied by molecular docking. RESULTS: Berberine 2.5 and 5.0 mg/kg could prolong the analgesic time of morphine. In acute and chronic morphine tolerance models, berberine could inhibit the decrease of MPAE and baseline latency(P tolerance model, berberine could rapidly reverse the decre

关键词： berberine morphine pain drug tolerance hyperalgesia

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Volumetric fluid analysis of fixed monthly anti-VEGF treatment in patients with neovascular age-related macular degeneration

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International Journal of Ophthalmology(English edition) 2023年第6期16卷 909-914页

作者： Bo Hee Kim In Boem Chang Hyeong Gon Yu In Hwan Hong Department of Ophthalmology Kangbuk Samsung HospitalSungkyunkwan University School of MedicineSeoul 03181Republic of Korea Seoul On Eye Clinic Seoul 03355Republic of Korea Department of Ophthalmology Seoul National University College of MedicineSeoul National University HospitalSeoul 03080Republic of Korea Retina Center Sky Eye InstituteSeoul 06536Republic of Korea Department of Ophthalmology Dongtan Sacred Heart HospitalHallym University Medical CenterHwaseong-siGyeonggido 18450Republic of Korea

·AIM:To evaluate visual outcomes and changes in fluid after administering monthly anti-vascular endothelial growth factor(VEGF)injections to treat neovascular agerelated macular degeneration(n AMD)with subretinal fluid(SRF)and pigment epithelial detachment(PED).·METHODS:This prospective study included eyes with n AMD previously treated with as-needed anti-VEGF *** patients were treated with six monthly intravitreal injections of *** volumetric segmentation analyses of the SRF and PED were *** main outcome measures included best-corrected visual acuity(BCVA),and SRF and PED volumes.·RESULTS:Twenty eyes of 20 patients were included in this *** the 6-month follow-up,BCVA and PED volume did not change significantly(P=0.110 and 0.999,respectively)but the mean SRF volume decreased from 0.53±0.82 mm3 at baseline to 0.08±0.23 mm3(P=0.002).The absorption rate of the SRF volume was negatively correlated with the duration of previous antiVEGF treatment(P=0.029).Seven of the 20 eyes(35%)showed a fluid-free macula and significant improvement in BCVA(P=0.036)by month 6.·CONCLUSION:Quantifying the SRF can precisely determine the patient’s responsiveness to anti-VEGF treatment of n AMD.

关键词： neovascular age-related macular degeneration anti-vascular endothelial growth factor treatment drug tolerance persistent subretinal fluid volumetric fluid analysis

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Empirical Study on Chemotherapeutic Susceptibility of SP Cells in Human Pulmonary Adenocarcinoma Cell Line A549 in Vitro

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Clinical oncology and cancer researeh 2010年第4期7卷 265-270页

作者： Tong XIE Li LI De-seng LIU Nai-quan MAO Dan-rong LI Chuan-tian ZUO Ding-ming HUANG Department of Thoracic Surgery the Affiliated Cancer Hospital of Guangxi Medical University Nanning 530021 Guangxi Province China.

OBJECTIVE To explore the chemotherapeutic susceptibility of SP cells in human pulmonary adenocarcinoma cell line A549 and the possible mechanism of multidrug *** SP and non-SP （NSP） cells in the cell line A549 were isolated by fluorescence activated cell sorter. The susceptibility of SP and NSP cells to DDP, 5-FU, VP16, NVB and GEM was detected by a drug susceptibility test, and IC50s were calculated 24 h a er the chemotherapy; and then a er a 2-hour IC50 treatment with 5 chemotherapeutic drugs on the 2 subsets of NSP cells, the intracellular drug levels were determined and analyzed using high performance liquid *** There was no statistical signifi cance in comparison of the di. erences in IC50s and in intracellular drug levels a er DDP treatment between the 2 subsets （P 〉 0.05）, （P 〉 0.05）. However,all IC50s of the other 4 drugs were significantly higher in the SP cells than in the NSP cells （P 〈 0.01）. A er the chemotherapy, the intracellular drug levels of the other 4 drugs were significantly lower in SP cells than in NSP cells （P 〈 0.01）.CONCLUSION Compared to NSP cells, SP cells in the cell line A549 have stronger resistance to the chemotherapeutics. The multidrug resistance of SP cells closely correlates with the function of SP cells discharging chemotherapeutic agents.

关键词： neoplastic stem cells abcg2 protein human drug resistance multiple intracellular space drug tolerance

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Evaluation of Targeted Therapy for Locally Advanced or Metastatic Renal Cell Carcinoma in Tunisia

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Open Journal of Gastroenterology 2016年第7期6卷 197-204页

作者： Khaled Ben Ahmed Amira Daldoul Ghassen Tlili Laila Ben Fatma Olfa Gharbi Mahdi Afrit Jihene Fkih Hammouda Boussen Mounir Frikha Faouzi Mosbah Slim Ben Ahmed Department of Urology Sahloul University Hospital Sousse Tunisia Department of Medical Oncology Farhat Hached University Hospital Sousse Tunisia Department of Medical Oncology Abderrahman Mami University Hospital Ariana Tunisia Department of Medical Oncology Habib Bourguiba University Hospital Sfax Tunisia

Introduction: Renal cell carcinoma (RCC) is known to be chemo resistant but with the introduction of targeted therapies;there has been a “revolution” in its treatment strategies. The only targeted therapy available in Tunisia for the treatment of metastatic and/or locally advanced RCC is sunitinib. Objective of the Study: To evaluate therapeutic results and tolerance of sunitinib in metastatic and/or locally advanced RCC. Subjects and Methods: This was a retrospective study covering a period of six years (from January 2008 to January 2014) conducted in 5 medical oncology departments in Tunisia. The population of the study consisted of 29 patients treated with sunitinib for metastatic and/or locally advanced RCC. Results: The mean age of patients was 51 years. Three patients had tumor recurrence and 26 patients had a metastatic RCC. The prognosis was good for 5 patients, intermediate for 19 patients and poor for 5 patients. The median duration of treatment was 5 months. Because of side effects, treatment was discontinued in 12.5% of cases and the dose was reduced in 10.3% of cases. Side effects consisted of asthenia (95.8%), stomatitis (70.8%), anemia (50%), hand-foot syndrome (55.8%) in addition to nausea and vomiting (54.2%). Objective response was observed in 37.5% of patients after 3 months of treatment and in 50% after 6 months. The median progression-free survival was 14 months (95% CI, 7.9 to 20.6). The median overall survival was 22 months (95% CI, 15.6 to 28.7). Conclusion: The prognosis of RCC in Tunisian patients has clearly improved with the introduction of sunitinib, but other therapies with a proven efficacy as a first and second line therapy should be considered.

关键词： Renal Cell Carcinoma Metastasis Molecular Targeted Therapy Sunitinib drug tolerance

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Establishment and Validation of an Anoiki-Associated Gene Signature Capable of Predicting Hepatocellular Carcinoma-related Immune Cell Infiltration and Prognostic Outcomes

Establishment and Validation of an Anoiki-Associated Gene Si...

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浙江省免疫学会第十三次学术大会

作者： Junjie Ni Lutong Liu Pu Wu Xiaotao Zhu Chaoyang Xu

Background:Hepatocellular carcinoma(HCC) is extremely heterogeneous,abd predicting patient outcomes remains a *** is a distinct type of cell death that exerts an essential role in oncogenic invasion and *** presence of anti-anoikis factors contributes to cancer aggressiveness and drug *** and methods:HCC patient transcriptomic and clinical data were accessed using public databases.A least absolute shrinkage and selection operator(LASSO) Cox approach was used to define a multigenic prognostic biosignature in the Cancer Genome Atlas(TCGA) patients,after which International Cancer Genome Consortium(ICGC)patients were leveraged for validation of this *** overall survival(OS) of low-and high-risk patient groups was compared using Kaplan-Meier analyses,with univariate and multivariate approaches being employed to establish independent predictors related to patient OS.A single-sample gene-set enrichment analysis(ssGSEA) strategy was also used to calculate immune cell infiltration scores and immune pathway activity,while Kyoto encyclopedia of genes and genome(KEGG) and Hallmark enrichment was identified via GSEA ***:Through the use of a LASSO Cox regression approach,a 9-gene anoikis-related biosignature model was ***-risk patient OS was shorter relative to low-risk *** operating characteristic(ROC)curves confirmed the robust prognostic utility of this 9-gene biosignature,and risk scores were independently related to patient OS in a multivariate Cox *** differences in immune status were also detected between these groups,with greater enrichment of cancer-associated pathways in high-risk *** scores were significantly related to tumor staging,grading,and immune infiltration *** gene expression was also significantly related with tumor cell anti-tumor drug ***:These results highlight a novel signature comprised of 9 anoikis-relat

关键词： anoikis hepatocellular carcinoma prognostic model immune microenvironment drug tolerance

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