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Expression of the Grifola frondosa Trehalose Synthase Gene and Improvement of drought-tolerance in Sugarcane （Saccharum officinarum L.）

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Journal of Integrative Plant Biology 2006年第4期48卷 453-459页

作者： Shu-Zhen Zhang Ben-Peng Yang Cui-Lian Feng Ru-Kai Chen Jing-Ping Luo Wen-Wei cai Fei-Hu Liu State Key Laboratory of Tropical Crop Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China Institute of Tropical Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China Key Laboratory of Ecophysiology and Genetic Improvement for Sugarcane Ministry of Agriculture Fuzhou 350002 China College of Life Sciences Yunnan University Kunming 650091 China

Trehalose Is a nonreduclng dlsaccharlde of glucose that functions as a protectant In the stabilization of blologlcal structures and enhances stress tolerance to abiotic stresses in organisms. We report here the expression of a Grlfola frondosa trehalose synthase （TSase） gene for Improving drought tolerance In sugarcane （Saccharum offlclnarum L.）. The expression of the transgene was under the control of two tandem copies of the CaMV35S promoter and transferred Into sugarcane by Agrobacterium tumefaciens EHA105. The transgenlc plants accumulated high levels of trehalose, up to 8.805-12.863 mg/g fresh weight, whereas It was present at undetectable level in nontransgenlc plants. It has been reported that transgenlc plants transformed with Escherlchla coil TPS （trehalose-6-phosphatesynthase） and/or TPP （trehalose-6-phosphate phosphatase） are severely stunted and have root morphologlc alterations. Interestingly, our transgenlc sugarcane plants had no obvious morphological changes and no growth Inhibition in the field. Trehalose accumulation in 35S-35S：TSase plants resulted In In- creased drought tolerance, as shown by the drought and the drought physiological Indexes, such as the rate of bound water/free water, plasma membrane permeability, malondlaldehyde content, chlorophyll a and b contents, and activity of SOD and POD of the excised leaves. These results suggest that transgenlc plants transformed with the TSase gene can accumulate high levels of trehalose and have enhanced tolerance to drought.

关键词： drought tolerance Grifola frondosa Saccharum officinarum trehalose synthase gene.

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Improving drought tolerance of Casuarina equisetifolia seedlings by arbuscular mycorrhizas under glasshouse conditions

Improving drought tolerance of Casuarina equisetifolia seedl...

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第九届中国林业青年学术年会

作者： K.Pinyopusarerk CSIRO Plant Industry

Effects of mycorrhizal association on improving tolerance of host plant under stress environments have received attentions in recent *** this paper,six isolates of AMF （arbuscular mycorrhizal fungus） were inoculated to Casuarina equisetifolia seedlings under glasshouse conditions to investigate the effects of AMF on growth and drought tolerance of host *** the six isolates which belong to Glomus showed high mycorrhizal colonization （88.5-96.0%） with *** *** were subjected to drought stress without watering for seven days and survival of the seedlings inoculated with Glomus caledonium Gc90068,*** Gv9004 and *** Gc90036 increased by 36.6%,23.3%and 16.6%respectively compared with uninoculated *** influence of AMF on seedling height growth was found,but the effects of AMF on total biomass increment were very significant;the increment ranged from 25.7%to 118.9%compared with uninoculated treatment,and it was noted that AMF exerted more influences on root biomass than shoot *** on the changes in physiological and biochemical parameters among different treatments caused by drought stress,it was concluded that AM（arbuscular mycorrhizal） associations improve the drought tolerance of *** seedlings by means of some physiological and biochemical responses,such as lowering permeability of plasma membrane and MDA（malondialdehyde） contents,enhancing concentrations of P nutrition,soluble sugar,soluble protein and activities of POX（Peroxidase） of *** seedlings.

关键词： arbuscular mycorrhiza Casuarina equisetifolia physiological and biochemical responses drought tolerance

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The characteristics in phenotype and drought tolerance of the transgenic tobacco plants by induced overexpression of TaEXPB23 gene from wheat

The characteristics in phenotype and drought tolerance of th...

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从植物科学到农业发展——2012全国植物生物学大会

作者： Feng Li,Yangyang Han,Yanan Feng,Shichao Xing,Meirong Zhao,Jin Zhang, Wei Wang~* State Key Laboratory of Crop Biology,Shandong Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Tai’an,Shandong,271018.

Expansins are the key regulators of cell wall extension during plant *** our previous study,the drought tolerance of transgenic tobacco plants was improved by overexpressing a wheat expansin gene TaEXPB23 driven by a constitutive 35S cauliflower mosaic virus (CaMV) promoter,but the development and phenotype of transgenic plants were severely retarded under normal *** this paper,we found that the cellular characteristics and carbohydrate metabolism were changed in 35S::TaEXPB23 transgenic tobacco plants. Then,the suitability of expressing TaEXPB23 driven by a stress-inducible RD29A promoter in order to improve the phenotype of the transgenic tobacco plants was *** determination of physiological and biochemical parameters indicated that RD29A::TaEXPB23 transgenic tobacco plants exhibited enhanced tolerance in comparison with wild-type *** study demonstrates that the use of stress-inducible promoter, such as RD29A,may minimize the unfavorable phenotype caused by constitutive expression of transgene and improve the drought tolerance.

关键词： drought tolerance Expansin Stress-inducible promoter Transgenic plant

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The osmolyte-producing endophyte Streptomyces albidoflavus OsiLf-2 induces drought and salt tolerance in rice via a multi-level mechanism

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The Crop Journal 2022年第2期10卷 375-386页

作者： Shuqi Niu Yan Gao Huixian Zi Ying Liu Xuanming Liu Xianqiu Xiong Qingqing Yao Ziwei Qin Ning Chen Liang Guo Yuanzhu Yang Peng Qin Jianzhong Lin Yonghua Zhu Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation College of BiologyHunan UniversityChangsha 410008HunanChina Yuan Longping High-Tech Agriculture Co. Ltd.Changsha 410001HunanChina

drought and salinity are major environmental stresses that impair crop growth and productivity worldwide. Improving drought and salt tolerance of crops with microbial mutualists is an effective and environmentally sound strategy to meet the demands of the ever-growing world population. In the present study, we found that the Streptomyces albidoflavus Osi Lf-2, a moderately salt-tolerant endophytic actinomycete, produced abundant osmolytes, including proline, polysaccharides, and ectoine. Inoculation with Osi Lf-2 increased the osmotic-adjustment ability of the rice host by increasing the proline content(by250.3% and 49.4%) and soluble sugar(by 20.9% and 49.4%) in rice under drought and salt conditions, relative to the uninoculated control. Osi Lf-2 increased stress responses in the rice host at the physiological and biochemical levels(photosynthesis efficiency, osmolytes and antioxidant content), and the gene level(osmolytes synthesis, stress-responsive and ion-transport related genes), raising rice yields under both greenhouse and saline–alkaline soil conditions. The use of endophytic actinomycetes offers a promising biotechnological approach to developing stress-tolerant plants.

关键词： Rice Endophytic actinomycete Osmolytes Salt tolerance drought tolerance

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Improvement of drought tolerance in Cotton Expressing Exogenous ZmPIS Gene

Improvement of Drought Tolerance in Cotton Expressing Exogen...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Juan Wang, Kewei Zhang* School of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China

＜正＞The phospholipid signaling pathway （PI pathway） plays a key role in plant growth, development and responses to environmental stress conditions. Phosphatidylinositol

关键词： cotton drought tolerance phospholipid signaling ZmPIS

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Overexpression of an Arabidopsis Thaliana RGSl Confers ABA Hypersensitivity and drought tolerance

Overexpression of an Arabidopsis Thaliana RGSl Confers ABA H...

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长三角现代植物生物学与农业专题论坛

作者： Yun Chen, Fangfang Ji, Hong Xie, Jiansheng Liang (College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, People'' s Republic of China, 225009)

＜正＞Regulator of G-protein signaling （RGS1） proteins identified recently in Arabidopsis has been involved in the regulation of several physiological processes, but largely we know nothing about their roles at both physiological level and molecular level. In this experiment, we used the overexpression approach to present evidence that RGS1 protein played critical roles in plant development and in modulating ABA and drought stress signal transduction. RGS1 affected the shapes of leaves, the development of floral buds, the elongation of stems, siliques and hypocotyls, and the time of flowering. Post-germination growth of 35S-RGS1 transgenic plants was inhibited by 1μM ABA. RGS1 overexpression was hypersensitive to ABA in assays of root growth and confers lower water loss rate assayed with detached leaves and more drought tolerance. RT-PCR results indicated that RGS1 overexpresssion significantly stimulated the expression of NCED and ABA2, two genes encoding two key enzymes catalyzing ABA biosynthesis. Furthermore, the expression of several stress-regulated genes was either up-regulated or down-regulated in the RGS1 overexpression transgenic plants. Combined the results above and our previous results, we suggested that RGS1 exert its effects on plant responsiveness to ABA and drought tolerance largely through changing the expression of genes responsible either for ABA biosynthesis, and thus leading to changes in endogenous ABA levels, or for stress-responsive genes

关键词： ABA signaling Arabidopsis AtRGS1 protein drought tolerance RGS1 overexpression

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R2R3-MYB Protein GhMYB36 from Cotton Confers drought tolerance in Arabidopsis

R2R3-MYB Protein GhMYB36 from Cotton Confers Drought Toleran...

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江苏省遗传学会2017年学术研讨会——“技术创新与遗传...

作者：刘廷利 Jialiang Kan Xiaoshuang Liu Fan Liu Xitie Ling Tianzi Chen Yuwen Yang Jinyan Wang 张保龙 Provincial Key Laboratory of Agrobiology Jiangsu Academy of Agricultural Sciences

MYB transcription factors（TFs） are a large group of functionally diverse proteins and present in all eukaryotes. In plants, R2R3-MYB proteins are predominant among MYB TFs but their function in cotton remains largely unclear. In our study, we isolated GhMYB36, a gene encoding a R2R3-type MYB protein in Gossypium hirsutum, that is highly induced by PEG treatments. In addition, GhMYB36-silenced cotton plants were more sensitive to drought and exhibited greater malondialdehyde（MDA） content and lower total antioxidant activity than the control plants. GhMYB36 transgenic Arabidopsis plants showed better drought tolerance. The agro-infiltration-based transient expression of fused GhMYB36-GFP in tobacco cells indicated that GhMYB36 was located in the cell nucleus. Collectively, our results suggest that GhMYB36 functions as a R2R3-type MYB protein and is essential to the improvement of drought tolerance in plants.

关键词： transcription factors drought tolerance transgenic plants transient expression R2R3-type MYB proteins

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Heterologous expressing the isopentenyl transferase(ipt) gene directed by senescence associated receptor protein kinase(SARK)promoter improves drought tolerance of transgenic soybean

Heterologous expressing the isopentenyl transferase(ipt) gen...

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2011全国植物生物学研讨会

作者： Weiwei Huo~1,Xiaomei Zhang~1,Jing Chen~1,Fawei Wang~2,Nan Wang~2,Na Yao~2, Yanfang Wang~2,Xiuming Liu~2,Jing Yang~2,Haiyan Li~(1,2*) 1 College of Life Science,Jilin Agricultural University,Changchun,Jilin 130118,China 2 Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University,Changchun,Jilin 130118,China

drought,the most prominent threat to agricultural production worldwide, accelerates leaf senescence,leading to a decrease in canopy size,loss in photosynthesis and reduced quality and *** is an important crop in the world,however,its yields was clearly influenced by the ***, cultivating anti-aging and anti-drought soybean varieties is very important for economy and *** isopentenyl transferase(ipt) gene is an enzyme that

关键词： drought tolerance ipt gene SARK promoter soybean

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Diverse functions of phosphorylation contributes to drought tolerance in Nostoc flagelliforme facilitated by comparative phosphoproteomic

Diverse functions of phosphorylation contributes to drought ...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Fengkun Yan Lingxia Wang Xiaoxu Li Miaomiao Ding Meng Wang Jun Wang Wenyu Liang School of Agriculture Ningxia University School of Life Sciences Ningxia University College education for Nationalities Ningxia University

Terrestrial cyanobacteria originate from aquatic *** must form a unique mechanism of drought adaptation during the long term ***,the mechanism remains *** this study,we carried out a global and sitespecific phosphoproteomic analysis on Nostoc flagelliforme,a kind of terrestrial cyanobacteria being strong drought ecological *** total,329 phosphopeptides and 1168 phosphorylation sites from 224 proteins were identified through the combined use of protein/peptide prefractionation,TiO2 enrichment,and LC-MS/MS *** identified phosphoproteins were functionally categorized and found to be involved in various biological processes such as photosynthesis,ROS scavenging,cell wall synthesis and two-component signaling *** results revealed that the content of photosynthesis products,sucrose,trehalose and soluble sugar were significantly *** saccharides play a key role in osmotic *** also found that the content of glycogen was significantly *** result suggests that glycogen could serve as a supportive rather than a competitive carbon pool for the synthesis of *** treatment caused an increase in activity of antioxidant enzymes like Trx,CAT,APX and MDHAR with water *** data provide the first global survey of phosphorylation in terrestrial cyanobacteria by using phosphoproteomic *** provided data may help to reveal the physiological functions underlying phosphorylation and entire signaling networks in terrestrial cyanobacteria.

关键词： Nostoc flagelliforme phosphoproteomic photosynthesis drought tolerance

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Panax ginseng PgTIP1 gene confers enhanced salt and drought tolerance to transgenic soybean plants through maintaining homeotasis of water,salt ions and ROS

Panax ginseng PgTIP1 gene confers enhanced salt and drought ...

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第七届长三角植物科学研讨会暨青年学术报告会

作者： J.An C.Cheng Z.Hu H.Chen W.Cai B.Yu Lab of Plant Stress Biology College of Life SciencesNanjing Agricultural University Institute of Plant Physiology and Ecology Shanghai Institutes for Biological SciencesUniversity of Chinese Academy of SciencesChinese Academy of Sciences

Panax ginseng PgTIP1 gene was formerly demonstrated to possess high water channel activity by its heterologous expression in Xenopus laevis oocytes and in yeast;and also plays significant roles in enhanced salt/drought tolerance of Pg TIP1-transgenic Arabidopsis plants and salt adaptation of Pg TIP1-transformed Glycine *** this work,Pg TIP1 gene was further transformed into the hybrid strain 4076(F),which was selected for salt tolerance generation by generation from the cross assembly *** N23674×*** BB52 *** Pg TIP1-transgenic soybean plants mediated by the pollen tube pathway,represented by the lines L19 and L29,were simultaneously analyzed at the physiological and molecular levels for enhanced salt and drought *** results showed that,the salt-stressed Pg TIP1-transgenic L19 and L29 have acquired better leaf stomatal movement and water-gas exchange capacity,obvious drops of absorption,transport and accumulation of Na,Cland Na/K ratio,and enhanced antioxidase activities of SOD,POD,CAT,and APX in the roots or leaves in contrast to *** suffered from PEG-induced drought stress,the strengthened leaf water-retention capacity and the alleviated cell membrane damages of roots and leaves were displayed for Pg TIP1-transgenic soybean ***,the Pg TIP1-transgenic soybean lines could exhibit salt and drought tolerance together,which are not only related to the primary functions of Pg TIP1 gene involved in water status regulation,but also to its secondary roles in up-regulated expression of stress-related genes(Gm NHX1,Gm SOS1,Gm CLC1,Gm CAT1,Gm APX1,Gm NCED1 and Gm P5 CS1),which are involved in homeostasis of Na,K,and Cl,ROS scavenging,and synthesis of ABA and proline,etc.

关键词： PgTIP1 transformation salt tolerance drought tolerance transgenic soybean water relations ion homeostasis ROS scavenging

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