检索结果-南通市图书馆

Microsystems & Nanoengineering 2017年第1期3卷 323-331页

作者： Tatiana Trantidou Yuval Elani Edward Parsons Oscar Ces Department of Chemistry Imperial College LondonLondon SW72AZUK Institute of Chemical Biology Imperial College LondonLondon SW72AZUK London Centre for Nanotechnology University College LondonLondon WC1E 6BTUK

Polydimethylsiloxane(PDMS)is a dominant material in the fabrication of microfluidic devices to generate water-in-oil droplets,particularly lipid-stabilized droplets,because of its highly hydrophobic ***,its key property of hydrophobicity has hindered its use in the microfluidic generation of oil-in-water droplets,which requires channels to have hydrophilic surface *** this article,we developed,optimized,and characterized a method to produce PDMS with a hydrophilic surface via the deposition of polyvinyl alcohol following plasma treatment and demonstrated its suitability for droplet *** proposed method is simple,quick,effective,and low cost and is versatile with respect to surfactants,with droplets being successfully generated using both anionic surfactants and more biologically relevant *** method also allows the device to be selectively patterned with both hydrophilic and hydrophobic regions,leading to the generation of double emulsions and inverted double emulsions.

关键词： double emulsions droplet microfluidics hydrophobic hydrophilic PDMS surface modification

Recent advances in droplet microfluidics for microbiology

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第4期33卷 1729-1742页

作者： Ziyi He Hao Wu Xianghua Yan Wu Liu State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan430070China Interdisciplinary Sciences Institute Huazhong Agricultural UniversityWuhan 430070China State Key Laboratory of Agricultural Microbiology College of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China The Cooperative Innovation Center of Sustainable Pig Production Wuhan 430070China Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology Wuhan 430070China Institute of Pharmaceutical Analysis School of Pharmaceutical SciencesCheeloo College of MedicineShandong UniversityJinan 250012China

Advances in microbiology rely on innovations in technology. droplet microfluidics, as a versatile and powerful technique that allows high-throughput generation and manipulation of subnanoliter volume droplets, has become an indispensable tool shifting experimental paradigms in microbiology. droplet microfluidics has opened new avenues to various microbiological research, from resolving single-cell heterogeneity to investigating spatiotemporal dynamics of microbial communities, from precise quantitation of microbiota to systematic decipherment of microbial interactions, and from isolating rare and uncultured microbes to improving genetic engineered strains. In this review, we present recent advances of droplet microfluidics in various fields of microbiology: i) microbial cultivation, ii) microorganism detection and characterization, iii) antibiotic susceptibility testing, iv) microbial interactions, v) microbial *** also provide our perspectives on the challenges and future directions for droplet microfluidic-based microbiology research.

关键词： droplet microfluidics Microorganisms Antibiotic susceptibility testing Microbial interactions Microbial biotechnology

droplet microfluidics for Highly Efficient Selection of Recognition Ligands

在线全文

学校读者我要写书评

暂无评论

Droplet Microfluidics for Highly Efficient Selection of Reco...

Crystal phase control and ignition properties of HNS/CL-20 composite microspheres prepared by microfluidics combined with emulsification techniques

第十届全国化学生物学学术会议

作者： Zhi Zhu Yanling Song Yuyu Tan Chaoyong Yang Department of Chemical Biology College of Chemistry and Chemical EngineeringXiamen University

The acquisition of biochemical information is the key step for human to explore the essence of life phenomena. Biosensing technology is an important tool for biochemical information acquisition. It provides an effective and quick analytical means to the major problems such as environment and food monitoring, disease diagnosis and treatment, biomedical research and biochemical threat *** ligands are the core of biological sensing, as well as the foundation of modern biological analysis and medical diagnosis. The common recognition ligands include small molecules, aptamers, peptides, antibodies, etc. Among them, aptamers and peptides are highly attractive due to their small molecular weight, chemical synthesis with easy modification, high affinity and specificity. However, both the SELEX process for aptamer generation and the phage display process for peptide generation are time-consuming, labor-intensive, inefficient, and expensive. droplet microfluidic techniques have recently been recognized as powerful tools because thousands of monodisperse microdroplets can be generated in a short time with molecules/cells individually encased, allowing high-throughput analysis and manipulation with precise control of the local environment. The encapsulation of each molecule from the selection library into individual droplets can help isolate the information from the big library and evaluate each binding ligand before sequencing,thus greatly reducing the time-consuming process. Herein, we will present our recent progress of using droplet microfluidics for aptamer and peptide selection.

关键词： droplet microfluidics aptamer peptide selection

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Particuology 2024年第2期85卷 241-251页

作者： Yi Liu Yunyan Guo Rui Zhu Jinqiang Zhou Zhengxu Lin Kai Han Chongwei An Jingyu Wang Bidong Wu School of Environment and Safety Engineering North University of ChinaTaiyuan030051China School of Engineering Beijing Institute of TechnologyBeijing100081China Shanxi Province Ultrafine Powder Engineering Technology Research Center Taiyuan030051China

Improved controllability and energy density of ignition agents are of great significance for the devel-opment of energetic composite *** this study,droplet microfluidics and emulsification tech-niques were combined to prepare HNS/CL-20 composite microspheres with polyglycidyl azide polymer(GAP)as the *** influence of binder content on the morphology of microspheres was investi-gated,and the microspheres were characterized and tested for particle size,crystal structure,thermal decomposition,dispersibility,mechanical sensitivity,combustion behavior and detonation *** results showed that microspheres prepared with a binder content of 3%had higher sphericity and particle size *** microspheres retained the crystal structure of both HNS and CL-20(ε-type).Compared with raw HNS,the microspheres had higher apparent activation energy,better safety per-formance,and good *** ignition experiments and detonation performance tests show that HNS/CL-20 composite microspheres have excellent ignition performance,obvious combustion flame,and significant energy release effects,which are expected to achieve high energy and high-speed response of the igniter,thus improving the ignition reliability in special environments or systems.

关键词： droplet microfluidics Emulsification technology Energetic materials Composite microspheres Crystal phase control Ignition

One-step rapid preparation of CL-20/TNT co-crystal assembly and spheroidized coating based on droplet microfluidic technology

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Defence Technology（防务技术） 2023年第9期27卷 251-262页

作者： Jiahui Shi Bidong Wu Jinqiang Zhou Dawei Ren Dongxu Zhang Chongwei An Jingyu Wang School of Environment and Safety Engineering North University of ChinaTaiyuan030051China School of Mechatronical Engineering Beijing Institute of TechnologyBeijing100081China

Energetic materials pose challenges in preparation and handling due to their contradictory properties of high-energy and *** emergence of co-crystal explosives is a new opportunity to change this *** the co-crystal explosive is coated into spherical particles with uniform particle size distribution,this contradiction can be further ***,binder-coated hexanitrohexaazaisowurtzitane/2,4,6-trinitrotoluene(CL-20/TNT)co-crystal microspheres were prepared by droplet microfluidic technology in this *** coating effects of different binder formulations of nitrocellulose(NC)and NC/fluorine rubber(F2604)on the co-crystal spheres were *** scanning electron microscopy(SEM)results showed that the use of droplet microfluidic technology with the above binders can provide co-crystal microspheres with regular spherical morphology,uniform particle size distribution and good dispersion.X-ray diffraction(XRD),fourier-transform infrared(FT-IR),differential scanning calorimetry(DSC)and thermo-gravimetric(TG)methods were employed to compare the properties of the co-crystal microspheres,raw material and pure *** formation of CL-20/TNT co-crystal in the microspheres was confirmed,and the co-crystal microspheres exhibited better thermal stability than the raw material and pure *** addition,the mechanical sensitivity and combustion performance of the co-crystal microspheres were further *** results showed that the co-crystal microspheres were more insensitive than CL-20 and pure co-crystal,and displayed excellent self-sustained combustion performance and theoretical detonation *** study provides a new method for the fast,simple and one-step preparation of CL-20/TNT co-crystal microspheres,with binder coating,uniform particle size distribution,and excellent performance level.

关键词： droplet microfluidics CL-20 TNT Co-crystal Spherical coating

Integration of droplet Microfluidic Tools for Single-cell Functional Metagenomics:An Engineering Head Start

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2021年第3期19卷 504-518页

作者： David Conchouso Amani Al-Ma’abadi Hayedeh Behzad Mohammed Alarawi Masahito Hosokawa Yohei Nishikawa Haruko Takeyama Katsuhiko Mineta Takashi Gojobori Department of Industrial and Mechanical Engineering Universidad de las Américas PueblaPuebla 72810Mexico Computational Bioscience Research Center King Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia Biological and Environmental Sciences and Engineering Division King Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia Research Organization for Nano&Life Innovation Waseda UniversityTokyo 162-0041Japan Department of Life Science and Medical Bioscience Waseda UniversityTokyo 162-8480Japan Institute for Advanced Research of Biosystem Dynamics Waseda Research Institute for Science and EngineeringWaseda UniversityTokyo 169-8555Japan Computational Bio Big-Data Open Innovation Laboratory AIST-Waseda UniversityTokyo 169–0072Japan Computer Electricaland Mathematical Sciences and Engineering DivisionKing Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia

droplet microfluidic techniques have shown promising outcome to study single cells at high ***,their adoption in laboratories studying“-omics”sciences is still irrelevant due to the complex and multidisciplinary nature of the *** facilitate their use,here we provide engineering details and organized protocols for integrating three droplet-based microfluidic technologies into the metagenomic pipeline to enable functional screening of bioproducts at high ***,a device encapsulating single cells in droplets at a rate of~250 Hz is described considering droplet size and cell ***,we expand on previously reported fluorescence-activated droplet sorting systems to integrate the use of 4 independent fluorescence-exciting lasers(i.e.,405,488,561,and 637 nm)in a single platform to make it compatible with different fluorescence-emitting *** this sorter,both hardware and software are provided and optimized for effortlessly sorting droplets at 60 ***,a passive droplet merger is also integrated into our pipeline to enable adding new reagents to already-made droplets at a rate of 200 ***,we provide an optimized recipe for manufacturing these chips using silicon dry-etching *** of the overall integration and the technical details presented here,our approach allows biologists to quickly use microfluidic technologies and achieve both single-cell resolution and high-throughput capability(>50,000 cells/day)for mining and bioprospecting metagenomic data.

关键词： droplet microfluidics Single cell Metagenomics droplet sorter Biotechnology

Functional Surfactants for Molecular Fishing,Capsule Creation,and Single-Cell Gene Expression

在线全文

学校读者我要写书评

暂无评论

Nano-Micro Letters 2021年第9期13卷 377-385页

作者： Mohammad Suman Chowdhury Xingcai Zhang Leila Amini Pradip Dey Abhishek Kumar Singh Abbas Faghani Michael Schmueck‑Henneresse Rainer Haag Institut Für Chemie Und Biochemie Freie Universität BerlinTakustrasse 314195 BerlinGermany John A.Paulson School of Engineering and Applied Sciences Harvard UniversityCambridgeMA 02138USA School of Engineering Massachusetts Institute of TechnologyCambridgeMA 02139USA Berlin Institute of Health-Center for Regenerative Therapies CharitéUniversitätsmedizin Berlin-CVKFöhrer Str.1513353 BerlinGermany BerlinCenter for Advanced Therapies CharitéUniversitätsmedizin Berlin-CVKFöhrer Str.1513353 BerlinGermany

Creating a single surfactant that is open to manipulation,while maintaining its surface activity,robustness,and compatibility,to expand the landscape of surfactant-dependent assays is extremely *** report an oxidation-responsive precursor with thioethers and multiple 1,2-diols for creating a variety of functional surfactants from one parent *** these multifunctional surfactants,we stabilize microfluidics-generated aqueous *** droplets encapsulate different components and immerse in a bioinert oil with distinct interfaces where an azide-bearing surfactant allow fishing of biomolecules from the droplets,aldehyde-bearing surfactant allow fabrication of microcapsules,and hydroxyl-bearing surfactants,with/without oxidized thioethers,allow monitoring of single-cell gene *** multifunctional surfactants poses opportunities for broad applications,including adsorption,bioanalytics,catalysis,formulations,coatings,and programmable subset of emulsions.

关键词： Dendronized fluorosurfactants droplet microfluidics Microcapsules Oxidation-responsive fluorosurfactants Cell encapsulation

Measurement of copy number variation in single cancer cells using rapid-emulsification digital droplet MDA

在线全文

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2017年第1期3卷 260-266页

作者： Samuel C.Kim Gayatri Premasekharan Iain C.Clark Hawi B.Gemeda Pamela L.Paris Adam R.Abate Department of Bioengineering and Therapeutic Sciences University of CaliforniaSan Francisco(UCSF)California Institute for Quantitative Biosciences(QB3)San FranciscoSan FranciscoCA 94158USA Department of Urology Division of Hematology&OncologyUniversity of CaliforniaSan Francisco(UCSF)San FranciscoCA 94158USA

Uniform amplification of low-input DNA is important for applications across biology,including single-cell genomics,forensic science,and microbial and viral ***,the requisite biochemical amplification methods are prone to bias,skewing sequence proportions and obscuring signals relating to copy *** droplet multiple displacement amplification enables uniform amplification but requires expert knowledge of microfluidics to generate monodisperse *** addition,existing microfluidic methods are tedious and labor intensive for preparing many ***,we introduce rapid-emulsification multiple displacement amplification,a method to generate monodisperse droplets with a hand-held syringe and hierarchical droplet *** conventional microfluidic devices require >10 min to emulsify a sample,our system requires tens of seconds and yields data of equivalent *** demonstrate the approach by using it to accurately measure copy number variation(CNV)in single cancer cells.

关键词： amplification bias copy number variation ddMDA droplet microfluidics multiple displacement amplification

High-throughput droplet microfluidic synthesis of hierarchical metal-organic framework nanosheet microcapsules

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2019年第11期12卷 2736-2742页

作者： Songting Wu Zhong Xin Shicheng Zhao Shengtong Sun Shanghai Key Laboratory of Multiphase Materials Chermical Engineering School of Chemical Engineering East China University of Science and Technology 130 Meilong RoadShanghai 200237China Center for Advanced Low-dimension Materials National Engineering Resarch Center for Dyeing and Finishing of TextilsDonghua Unriversity2999 North Renmin RoadShanghai 201620China

Using two-dimensional(2D)metal-organic framework(MOF)nanosheets as new building blocks to create more complex architectures at the mesoscopic/macroscopic scale has attracted extensive interest in recent ***,it remains a great challenge to assemble MOF nanosheets into hierarchical hollow structures so *** this paper,we describe a successful example of hierarchical MOF nanosheet microcapsules,with precisely controlled sizes,produced on large scale within minutes with a continuous droplet microfluidic *** a reaction/diffusion growth mechanism,the microcapsule shells feature a continuous smooth inner layer and a porous outer *** hierarchical structure enables the encapsulation of magnetite nanoparticles inside and loading of dense gold nanoparticles outside the microcapsules,which exhibit highly efficient heterogeneous catalytic activity and easy *** present microfluidic assembly method offers a new pathway for preparing hierarchical MOF nanosheet structures,with the potential for extension to the formation of other 2D nanosheets in general.

关键词： metal-organic framework nanosheet droplet microfluidics microcapsule hierarchical structure

Crosstalk-free colloidosomes for high throughput single-molecule protein analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2020年第10期63卷 1507-1514页

作者： Kun Yin Xi Zeng Xuan Liang Haopai Wei Huimin Zeng Wei Qi Weidong Ruan Yanling Song Chaoyong Yang Zhi Zhu The Key Laboratory of Chemical Biology of Fujian Province the MOE Key L aboratory of Spectrochemical Analysis&InstrumentationState Key Laboratory of Physical Chemistry of Solid SurfacesCollaborative Innovation Center of Chemistry for Energy MaterialsDepartment of Chemical EngineeringDepartment of Chemical BiologyCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China

droplet microfluidics is a powerful platform for high-throughput single-molecule protein ***,the issues of coalescence and crosstalk of droplets compromise the accuracy of detection and hinder its wide *** address these limitations,a novel colloidosome-based method was presented by combining a Pickering emulsion with droplet microfluidics for single-molecule protein *** the self-assembly of easily synthesized colloidal surfactant F-SiO2 NPs at the water/oil interface,the colloidosomes are rigidly stabilized and can effectively avoid the leakage of fluorescent *** crosstalk-free colloidosomes enable high-throughput single-molecule protein analysis,including heterogenous dynamic studies and digital *** a robust and accurate method,colloidosome-based microfluidics is promising as a powerful tool for a wide variety of applications,such as directed enzyme evolution,digital enzyme-linked immunosorbent assay(ELISA),and screening of antibiotics.

关键词： colloidosomes droplet microfluidics crosstalk-free single-molecule analysis protein detection