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A New Phenylpropanoid Glycoside from dragon＇s blood of Dracaena cambodiana

Chemical Research in Chinese Universities 2013年第3期29卷 483-486页

作者： sHEN Hai-yan WANG Hui ZUO Wen-jian ZHAO You-xing LUO Ying MEI Wen-li DAI Hao-fu Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of Agriculture Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 P R. China Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine Haikou 571101 P. R. China Horticultural and Garden College Hainan University Haikou 570228 P. R. China

A new phenylpropanoid glycoside, named cambodianin F（1）, together with three known compounds, 1-O-β-D-glucopyranosyl-2-hydroxy-4-allylbenzene（ 2 ）, 1-O-（ 6-O-a-L-rhamnopyranosyl-β-D-glucopyranosyl）-2-hydroxy- 4-allylbenzene（3） and 1,2-di-O-β-D-glucopyranosyl-4-allylbenzene（4） was isolated from the dragon＇s blood of Dracaena cambodiana. The new compound was elucidated by HR-EsI-Ms and spectroscopic techniques（UV, IR, 1D and 2D NMR）.

关键词： dragon's blood Dracaena cambodiana Cambodianin F

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HPLC-EsI-Ms Analysis of Flavonoids Obtained from Tissue Culture of Dracaena cambodiana

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Chemical Research in Chinese Universities 2015年第1期31卷 38-43页

作者： WANG Hui WANG Jiayuan WU Xiaopeng sHEN Haiyan LUO Ying DAI Haofu MEI Wenli Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine Key Laboratory of Tropical Crop Biotechnology Ministry of Agriculture Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 P. R. China

dragon＇s blood is a traditional medicine and used in many countries with different cultures because of its various therapeutic properties. The main bioactive constituents of dragon＇s blood are flavonoids, which exhibit various pharmaceutical activities, such as haemostatic, analgesic, anticoagulant, and antimicrobial activities and so on, and have attracted the attention of researchers on the development of new drugs. However, the formation process of dragon＇s blood in nature is very slow, which cannot meet the demand of pharmaceutical uses. Dracaena cambodiana （D. cambodiana） is one of the resource plants of dragon＇s blood na, 6-benzylaminopurine was added in the medium, then red During the course of tissue culture of D. cambodiasecretion was discovered in the cultured medium. Twelve compounds（1--12）, including 11 flavonoids, were determined via the analysis of constituents of the cultured medium by high performance liquid chromatography-electrospray ionization-mass spectrometry（HPLC-EsI-Ms） and the results were compared with that of the standards isolated from dragon＇s blood. Among compounds 1-12, 4,4＇- dihydroxy-2-methoxydihydrochalcone（5）, 4,4＇-dihydroxy-2,6-dimethoxydihydrochalcone（6） and （2s）-7,3＇-dihydroxy- 4＇-methoxyflavane（9） were major compounds of the red secretion, with contents of 15.70, 7.10 and 57.23 mg/L, respectively. Therefore, it is promising that flavonoids from dragon＇s blood can be obtained from the tissue culture of its resource plants for the purpose of drug development.

关键词： Flavonoid dragon's blood High performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-EsI-Ms) Dracaena cambodiana Tissue culture

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