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Elite,transformable haploid inducers in maize

The Crop Journal 2024年第1期12卷 314-319页

作者： Brent Delzer Dawei Liang David Szwerdszarf Isadora Rodriguez Gonzalo Mardones Sivamani Elumalai Francine Johnson Samson Nalapalli Rachel Egger Erin Burch Kerry Meier Juan Wei Xiujuan Zhang Huaping Gui Huaibing Jin Huan Guo Kun Yu Yubo Liu Becky Breitinger Ana Poets Jason Nichols Wan Shi David Skibbe Qiudeng Que Timothy Kelliher Seeds Development Syngenta SeedsJanesvilleWI 53546USA Seeds Research Syngenta Biotechnology China Co.Ltd.Beijing 102206China Centro de Investigación de Cultivos Arica Syngenta R&DArica 1000000Chile Syngenta S.A. Proyecto la SoledadGraneros 2880000Chile Seeds Research Syngenta Crop Protection LLCRTPNC 27709USA Seeds Development Syngenta SeedsSlaterIA 50244USA

The introduction of alleles into commercial crop breeding pipelines is both time consuming and *** technologies that are disrupting traditional breeding processes are doubled haploid(DH)breeding and genome editing(GE).Recently,these techniques were combined into a GE trait delivery system called HI-Edit(Haploid Inducer-Edit).In HI-Edit,the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety,obviating recurrent *** HI-Edit to operate at scale,an efficient transformable HI line is needed,but most maize varieties are recalcitrant to transformation,and haploid inducers are especially difficult to transform given their aberrant reproductive *** marker assisted selection and a three-tiered testing scheme,we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable,have high haploid induction rates,and exhibit a robust,genetically-dominant anthocyanin native trait that may be used for rapid haploid *** show that transformation of these elite‘‘HI-Edit”lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic ***,we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk *** strategy and results of this study should facilitate the development of commercially scalable HI-Edit systems in diverse crops.

关键词： Zea mays L doubled haploids Transformation Genome editing QTL

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QTL for grain yield and yield parameters in winter wheat(Triticum aestivum L.)

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湖南农业大学学报（自然科学版） 2007年第S1期33卷 86-91页

作者： LIN Hai,GUO Hui-jun,ZHANG Xiu-ying,WANG Rui-xia,XIAO Shi-he(The National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Science,12 Zhongguancun South Street,Beijing,100081,P.R)

Field trials with a set of 108 doubled haploid lines(DHs) derived from a cross between the Chinese winter wheat ***9613 and H1488 were run at Beijing(China).Phenotypic data were recorded for major agronomic yield traits,*** weight per ear,grain number per ear and thousand grain weight(Tgw) in two field trials at *** on the phenotypic data and a genetic map comprising 168 SSR markers,an analysis of quantitative trait loci(QTL) was carried out for yield and yield parameters using the composite interval mapping(CIM) approach.A total of 14 QTL were detected for these traits across two *** of these QTL located on chromosomes 1A and 2B,respectively,exhibited pleiotropic *** showing pleiotropic effects will be very useful for understanding the homeologous relationships of QTL and designing an appropriate marker-assisted selection programme by multi-trait selection in order to accumulate("pyramide") favorable alleles at different loci.

关键词： doubled haploids Quantitative trait loci(QTL) Grain yield wheat(Triticum aestivum L.)

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The Effectiveness of Targeted Bulk Segregant Analysis to Identify AFLP Markers Closely Linked to QTL for Adult Plant Stripe Rust Resistance in Wheat

The Effectiveness of Targeted Bulk Segregant Analysis to Ide...

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第二届植物分子育种国际会议

作者： L.Herselman O.A.Philippou Z.A.Pretorius R.Prins Department of Plant Sciences University of the Free StatePO Box 339Bloemfontein9300South Africa Department of Plant Sciences University of the Free StatePO Box 339Bloemfontein9300South Africa Department of Plant Sciences University of the Free StatePO Box 339Bloemfontein9300South Africa Department of Plant Sciences University of the Free StatePO Box 339Bloemfontein9300South Africa CenGen （Pty） Ltd78 Fairbairn Street Worcester6850South Africa

＜正＞A ‘Kariega×Avocet S’ partial linkage map has made a significant contribution to understanding the genetics underlying the adult plant resistance （APR） to stripe rust, caused by Puccinia striiformis f. sp. t... 详细信息

关键词： Triticum aestivum doubled haploids Puccinia striiformis f. sp. tritici Phenotypic and marker data

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QTL for grain yield and yield parameters in winter wheat (Triticum aestivum L.)

QTL for grain yield and yield parameters in winter wheat (Tr...

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中国作物学会2007年全国作物遗传育种学术研讨会

作者： LIN Hai GUO Hui-jun ZHANG Xiu-ying WANG Rui-xia XIAO Shi-he~* (The National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Science,12 Zhongguancun South Street,Beijing,100081,P.R)

Field trials with a set of 108 doubled haploid lines(DHs)derived from a cross between the Chinese winter wheat ***9613 and H1488 were run at Beijing(China).Phenotypic data were recorded for major agronomic yield traits,*** weight per ear,grain number per ear and thousand grain weight(Tgw)in two field trials at Beijing. Based on the phenotypic data and a genetic map comprising 168 SSR markers,an analysis of quantitative trait loci (QTL)was carried out for yield and yield parameters using the composite interval mapping(CIM)approach.A total of 14 QTL were detected for these traits across two *** of these QTL located on chromosomes 1A and 2B, respectively,exhibited pleiotropic *** showing pleiotropic effects will be very useful for understanding the homeologous relationships of QTL and designing an appropriate marker-assisted selection programme by multi-trait selection in order to accumulate(“pyramide”)favorable alleles at different loci.

关键词： doubled haploids Quantitative trait loci(QTL) Grain yield wheat(Triticum aestivum L.)

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