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A Simple Method for Detection of Multiple Chemical-Specific IgGs in Serum Based on dot blotting

Health 2016年第15期8卷 1645-1653页

作者： Mayumi Tsuji Hsu-Sheng Yu Yasuhiro Ishihara Toyohi Isse Nami Ikeda-Ishihara Takuto Tuchiya Toshihiro Kawamoto Department of Environment Health University of Occupational and Environmental Health Kitakyusyu Japan Department of Food Science College of Agriculture National Pingtung University of Science and Technology Taiwan Laboratory of Molecular Brain Science Graduate School of Integrated Arts and Sciences Hiroshima University Higashi-Hiroshima Japan Division of Gene Research Natural Science Center for Basic Research and Development Hiroshima University Higashi-Hiroshima Japan

Plastic resins are known to cause occupational allergies. Therefore, serum-specific antibodies against plastic resins have been widely investigated as diagnostic markers for occupational allergies. In this study, we aimed to establish a convenient method for detection of multiple chemical-specific IgG antibodies in human serum based on dot blot analysis. Toluene diisocyanate (TDI), phthalic anhydride (PA), and formaldehyde (FA), which are frequently used to synthesize various resins, reacted well with lysine residues of human serum albumin (HSA) under alkaline conditions. Native polyacrylamide gel electrophoresis (PAGE) showed that the structures of chemical adducts of HSA were different from those of native HSA. Therefore, we performed dot blot assays using these adducts as artificial antigens. Serum samples from workers at plants utilizing plastic resins strongly reacted with TDI, PA, and FA adducts in HSA, while reduced signals were detecting using the serum from unexposed workers. These results suggested that dot blot assays using chemical-HSA adducts as antigens could be beneficial for simultaneously measuring multiple chemical-specific IgGs.

关键词： IgG dot blot assay Plastic Resin

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