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Chemical Property and Antibacterial Activity of Metronidazole-decorated Ti through Adhesive Dopamine

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Journal of Wuhan University of Technology(Materials Science) 2019年第4期34卷 968-972页

作者：付东杰 LU Yi GAO Shaohuai PENG Youjian 段海英 Department of Stomatology Renmin Hospital of Wuhan UniversityWuhan 430060China The State Key Laboratory Breding Base of Basic Science of Stomatology(Hubei-MOST) Wuhan 430079China Wuhan Centural Chinese Construction Third Engineering Bureau Wuhan 430022China

In order to present a novel metronidazole-decorated Ti implanted with antibacterial activity through adhesive Dopamine, and prepare metronidazole-decorated Ti, Ti substrates were pre-modified with Dopamine(DA) coating, and metronidazole was subsequently immobilized onto the surface of DA-modified Ti substrates. The prepared MET-decorated Ti interfaces were prepared for surface characterization tests:contacting angle measurements, scanning electron microscope, ATR-FTIR spectroscopy and in vitro anti-biofilm formation assay. Statistical tests were performed using by SPSS16.0. Contacting angle measurement, scanning electron microscopy, and ATR-FTIR spectroscopy confirmed the successful presence of MET grafted on the Ti surface. In addition, the MET-decorated Ti induced good in vitro antibacterial activity toward Escherichia coli and Streptococcus mutans,Gram-negative and Gram-positive bacteria, respectively. The modified Ti substrate with enhanced antibacterial activity holds a great potential as implanting material for applications in dental and bone graft substitutes.

关键词： Dopamine metronidazole-decorated antibacterial activity

Effects of Dopamine D_(1) receptor blockade on the ERG b- and d-waves during blockade of ionotropic GABA receptors

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Eye and Vision 2016年第1期3卷 245-252页

作者： Elka Popova Momchil Kostov Petia Kupenova Department of Physiology Medical University of SofiaSofiaBulgaria

Background:Some data indicate that the Dopaminergic and GABAergic systems interact in the vertebrate retina,but the type of interactions is not well understood.Methods:In this study we investigated the effect of Dopamine D_(1) receptor blockade by 75μM SCH 23390 on the electroretinographic ON(b-wave)and OFF(d-wave)responses in intact frog eyecup preparations and in eyecups where the ionotropic GABA receptors were blocked by 50μM picrotoxin.Student’s t-test,One-way repeated measures ANOVA with Bonferroni post-hoc test and Two-way ANOVA were used for statistical evaluation of the data.Results:We found that SCH 23390 alone significantly enhanced the amplitude of the b-and d-waves without altering their latency.The effect developed rapidly and was fully expressed within 8-11 min after the blocker application.Picrotoxin alone also markedly enhanced the amplitude of the ERG ON and OFF responses and increased their latency significantly.The effect was fully expressed within 25-27 min after picrotoxin application and remained very stable in the next 20 min.The effects of SCH 23390 and picrotoxin are similar to that reported in our previous studies.When SCH 23390 was applied on the background of the fully developed picrotoxin effect,it diminished the amplitude of the b-and d-waves in comparison to the corresponding values obtained during application of picrotoxin alone.Conclusion:Our results demonstrate that the enhancing effect of D_(1) receptor blockade on the amplitude of the ERG b-and d-waves is not evident during the ionotropic GABA receptor blockade,indicating an interaction between these neurotransmitter systems in the frog retina.We propose that the inhibitory effect of endogenous Dopamine mediated by D_(1) receptors on the ERG ON and OFF responses in the frog retina may be due to the Dopamine-evoked GABA release.

关键词： Dopamine D_(1)receptors GABA Electroretinogram b-wave d-wave

Effect of Cardioxane and Selenium on Lipoperoxidation and Levels of Dopamine in Rat Brain and Heart

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Neuroscience & Medicine 2019年第4期10卷 354-368页

作者： David Calderón Guzmán Norma Osnaya Brizuela Gerardo Barragán Mejía Hugo Juárez Olguín Lulú Sánchez Reyes Armando Valenzuela Pereza Norma Labra Ruiz Daniel Santamaría del Angel Laboratory of Neurosciences Instituto Nacional de Pediatría (INP) Mexico City Mexico Laboratory of Experimental Bacteriology INP Mexico City Mexico Laboratory of Pharmacology INP Mexico City Mexico Faculty of Medicine Universidad Nacional Autónoma de Mexico Mexico City Mexico.

Background: Cardioxane has been probed in patients with advanced malignancies to protect the heart. Selenium, an essential micronutrient exerts varieties of functions such as antioxidant. The aim of this study was to test if cardioxane (CDX) and selenium (Se) have additive antioxidant protective effect on brain and heart, and their relation with Dopamine levels. Methods: Thirty-six male Wistar rats divided in groups of 6 animals each, were treated as follows: G1, saline solution 0.9% (control);G2, 100 mg/kg of CDX;G3, 60 μg/kg of Se;G4, 20 mg/kg of 3-nitropropionic acid (3NP);G5, 3NP + CDX and G6, 3NP + Se. 3NP was used as an oxidative stress inducer. Drugs were administered intraperitoneally for 5 days. The animals were sacrificed on the last day of treatment and the brain and heart were extracted and used to measure lipid peroxidation, Dopamine, glutathione (GSH), ATPase, calcium, and H2O2. Results: In G2 and G5, Dopamine decreased in cortex and striatum while GSH increased in heart, cortex and cerebellum/medulla oblongata. ATPase activity increased in heart and cortex of groups 2, 3, 5 and 6. Lipoperoxidation and H2O2 increased in cortex of animals treated with 3NP. Conclusion: These results suggest that CDX increases antioxidant capacity in the brain and heart while selenium promotes alteration in Dopamine metabolism in view of the capacity of 3NP to generate free radicals.

关键词： Brain Dopamine Heart Cardioxane Selenium 3-Nitropropionic Acid

Simultaneous Determination of Epinephrine, Noradrenaline and Dopamine in Human Serum Samples by High Performance Liquid Chromatography with Chemiluminescence Detection

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Chinese Journal of Chemistry 2007年第7期25卷 942-946页

作者：陈福南张迎雪章竹君 College of Chemistry and Chemical Engineering Southwest University Chongqing 400715 China Department of Chemistry Qiongzhou University Qiongzhou Hainan 572200 China

A simple, rapid and accurate high performance liquid chromatographic （HPLC） technique coupled with chemiluminescence （CL） detection was developed for the simultaneous determination of epinephrine （E）, noradrenaline （NA） and Dopamine （DA）. It was based on the analyte enhancement effect on the CL reaction between luminol and potassium ferricyanide. The effects of various parameters, such as potassium ferricyanide concentration, luminol concentration, pH value and component of the mobile phase on chromatographic behaviors of the analytes （E, NA and DA） were investigated. The separation was carded out on C18 column using the mobile phase of 0.01 mol/L potassium hydrogen phthalate solution and methanol （92 ： 8, V/V）. Under the optimum condi- tions, E, NA and DA showed good linear relationships in the range of 1 × 10^-8 -5 × 10^-6, 5.0× 10^-9 -1.0× 10^-6 and 5.0×10^-9-1.0× 10^-6 g]mL respectively. The detection limits for E, NA and DA were 4.0×10^-9, 1.0× 10^-9 and 8.0 × 10^-10 g/mL. The proposed method has been applied successfully to the analysis of E, NA and DA in human serum samples.

关键词： high performance liquid chromatography chemiluminescence noradrenaline epinephrine Dopamine

Modulation of axonal ion channels and neuronal signaling by serotonin and Dopamine receptors

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Modulation of axonal ion channels and neuronal signaling by ...

Surface-enhanced Resonance Raman Spectroscopy sensor for rapid and sensitive detection of Dopamine

中国神经科学学会第十二届全国学术会议

作者： Yousheng Shu State Key Laboratory of Cognitive Neuroscience and Learning Beijing Normal University

Voltage-gated Na and K channels distributed at the axon are critical for action potential(AP) generation and propagation. We examined whether the axonal channels are subjected to modulation by neuromodulators and their receptors. For axonal Na channels, we performed patch-clamp recording at the axon of layer-5 pyramidal neurons in slices of prefrontal cortex(PFC) and found surprisingly that activation of 5-HT receptors selectively inhibits axon initial segment(AIS) but not axon-trunk Na currents. Moreover, channel subtype Na V1.2 but not Na V1.6 at the AIS is selectively modulated by 5-HT receptors. Consistently, dual somatic and axonal recording and simulation results demonstrate that the activation of 5-HT receptors could decrease the success rate of AP backpropagation toward the soma and dendrites. These results reveal a selective modulation of Na V1.2 distributed at the proximal AIS region and AP backpropagation by 5-HT receptors, suggesting a potential mechanism for serotonergic regulation of functional polarization in the dendro-axonal axis and synaptic plasticity. For axonal K channels, we found that activation of Dopamine D receptor decreased axonal K currents, whereas activation of D receptors showed an opposite effect. Further experiments demonstrated that functional D receptors were expressed at the main axon trunk and their activation could broaden the waveforms of axonal APs. Together, our results show that serotonin and Dopamine can regulate neuronal signaling by modulating axonal Na and K channels.

关键词： pyramidal neuron axon action potential 5-HT Dopamine sodium channel potassium channel

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Surface-enhanced Resonance Raman Spectroscopy sensor for rap...

Dopamine and cognitive function after global cerebral ischemia-reperfusion:a brief review

第十九届全国光散射学术会议

作者： Pan Li Xiaomin Cao Liangbao Yang Institute of Intelligent Machines Institution Chinese Academy of Sciences

＜正＞It is a challenge to develop a robust sensor for simple and rapid operation and sensitive detection of neurotransmitter in the complex specimen.Herein,the ferric-citrate-functionalized Au nanoparticles[Au NPs/

关键词： Acupuncture needle Dopamine Surface-enhanced Resonance Raman Spectroscopy

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Journal of Translational Neuroscience 2018年第3期3卷 9-16页

作者： Wenzhu Wang Xu Liu Yan Yu Lixu Liu School of Rehabilitation Medicine Capital Medical UniversityBeijing 100069China China Rehabilitation Research Center Beijing 100068China China Rehabilitation Science Institute Beijing 100068China Beijing Key Laboratory of Neural Injury and Rehabilitation Beijing 100068China Center of Neural Injury and Repair Beijing Institute for Brain DisordersBeijing 100069China

Global cerebral ischemia/hypoxia may occur due to various causes such as cardiac arrest,shock,and asphyxiation.Even though the patient’s life may be saved after cardiopulmonary resuscitation,cerebral ischemia– reperfusion injury is likely to occur and often results in neurological dysfunction.Apart from motor and speech impediments,patients with such injury may also suffer from impaired higher-level cognitive functions such as learning and memory,placing a heavy burden on families and society.Brain areas associated with the limbic system include the hippocampus,corpus striatum,and amygdala,which are linked with cognitive function.Those brain regions are easily damaged by hypoxia,and since they are connected with the Dopaminergic pathway,global cerebral ischemia–reperfusion can damage the Dopaminergic pathway as well and affect the projection of Dopaminergic neurons in the limbic system.This review article examines the feasibility of using Dopamine,a neurotransmitter heavily involved in cognitive function,in experimental research and clinical treatment of global cerebral ischemia–reperfusion injury.Specifically,we examine the effects of Dopamine on post-injury cognition and neuronal plasticity,with the ultimate goal of identifying a new tool for clinical treatment.

关键词： Dopamine hippocampus global cerebral ischemia–reperfusion injury cognition

Electrocatalytic oxidation and determination of Dopamine at a carbon ionic liquid electrode modified with nafion-L-aspartic acid composite film

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Journal of Pharmaceutical Analysis 2010年第1期22卷 1-6页

作者： Xiao-Dong Shangguan,Jian-Bin Zheng,Qing-Lin Sheng,Ya-Ping He Institute of Analytical Science,Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry,Northwest University,Xi’an 710069,China InstituteofAnalyticalScience ShaanxiProvincialKeyLaboratoryofElectroanalyticalChemistryNorthwestUniversityXi'an710069China

The electrocatalytic oxidation of Dopamine(DA)was studied by electrochemical approaches at a carbon ionic liquid electrode(CILE)modified with the composite film of nafion and L-aspartic acid(NL-CILE).The CILE was fabricated by replacing non-conductive organic binders with a room-temperature hydrophobic ionic liquid,1-butyl-3-methyl-imidazolium hexafluorophosphate.The composite film of NL was used as matrix to adsorb DA and catalyze the oxidation of DA in phosphate buffer solution(PBS).The electrochemical response of DA was investigated at the NL-CILE,the traditional carbon paste electrode(TCPE),CILE and the nafion modified CILE(N-CILE)in 0.1M PBS(pH 7.4),respectively.The results showed the superiority of NL-CILE to N-CILE,CILE and TCPE in terms of provision of higher sensitivity,faster electron transfer and better reversibility.Under optimum condition,the oxidation peak current was rectilinear with DA concentration range from 0.1μM to 0.1mM,with a detection limit of 0.03μM(S/N=3)by differential pulse voltammetry.The proposed method was applied to determine DA in samples successfully.

关键词： voltammetry Ionic liquid nafion L-aspartic acid Dopamine

Increased vulnerability to cocaine addiction in mice lacking Dopamine D3 receptor

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中国药理学与毒理学杂志 2012年第3期26卷 444-445页

作者：宋睿 Eliot L.GARDNER Intramural Research Program National Institute on Drug AbuseBaltimoreMD 21224USA Institute of Pharmacology and Toxicology Academy of Military Medical Sciences

Neuroimaging studies using positron emission tomography suggest that reduced Dopamine(DA) D2 receptor(D2R) availability in the striatum is associated with increased vulnerability to drug addiction in humans and experimental animals.However,the role of D3R in the neurobiology of addiction remains unclear.Here we report that D3R-knockout(D3-/-) mice display enhanced cocaine(and sucrose) taking observed during the acquisition and maintenance of cocaine self-administration and enhanced motivation for cocaine(and sucrose) seeking observed during progressive-ratio cocaine self-administration and extinction from cocaine self-administration.This increased vulnerability to cocaine was accompanied by decreased DA response to cocaine secondary to increased basal levels of extracellular DA in the nucleus accumbens,suggesting that enhanced cocaine-taking and cocaine-seeking behavior could be a compensatory response to decreased cocaine reward in D3-/-mice.In addition,D3-/-mice also displayed up-regulation of DA transporter in the striatum,suggesting that a neuroadaptative change occurred D3-/-mice to restore elevated basal levels of extracellular DA.These findings,for the first time,suggest that deletion of D3R increases vulnerability to cocaine-taking and cocaine-seeking behavior.Thus,reduced D3R availability in the brain constitutes an important risk factor for the development of cocaine addiction.

关键词： Dopamine D3 receptor cocaine self-administration drug-seeking