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Separation of dopamine and Epinephrine by a Novel Electrophoresis Technique with Nafion Membrane as Separation Column

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Wuhan University Journal of Natural Sciences 2004年第1期9卷 102-104页

作者： FangCheng WuBing-liang ZhangWu-ming ZhouXing-yao CollegeofChemistryandMolecularSciences WuhanUniversityWuhan430072HubeiChina

A novel electrophoresis technique, in which a strip of perflurosulfonic-acid (nafion 117) membrane was used to replace the conventional separation column and liquid buffer solution within, was developed and employed to separate the mixture of dopamine and epinephrine under a low separation voltage of 100 V with quadruple pulses amperometry detection. It was showed that the so-called Nafion membrane electrophoresis could be one of very simple and easy method and has the potentiality to be used to separate and analyze some small organic biologic molecules. Key words nafion membrane - electrophoresis - electrochemical detector - dopamine - epinephrine CLC number O 657. 7 Foundation item: Support by the Foundation of Electroanalytical Chemistry Open Lab (203231510), Changchum, China.Biography: Fang Cheng (1971-), male, Lecture, Ph. D, research direction: Electroanalysis.

关键词： nafion membrane electrophoresis electrochemical detector dopamine epinephrine

Immunohistochemical localization of dopamine and norepinephrine receptors in human early placental villi

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Chinese Science Bulletin 1996年第14期41卷 1203-1206页

作者：张荣庆黄威权张崇理 State Key Lab. of Repro. Biology Institute of Zoology Chinese Academy of Sciences Beijing 100080 China Dept. of Biol. Sci. and Biotech. Tsinghua University Beijing 100084 China Dept. of Histology and Embryology Fourth Military Medical College Xi'an 710032 China

Evidence so far accumulated suggests that the human early placenta can be considered as a complicated neuroendocrine organt. Our previous reports have demonstrated that the human early placenta presents not only a variety of neuropeptides but also neurotransmitters such as 5-hydroxytryptamine (5-HT), dopamine (DA) and norepinephrine (NE). It has been shown that these neurotransmitters in human placenta may play an important role in the process of blastocyst implantation and maintenance of normal pregnancy. As far as we are aware, no information concerning the DA and/or NE receptors localized in

关键词： human placental villi norepinephrine receptor dopamine receptor anti-idiotypic antibody immunohistochemistry.

Ketamine use disorder:preclinical,clinical,and neuroimaging evidence to support proposed mechanisms of actions

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Intelligent Medicine 2022年第2期2卷 61-68页

作者： Leah Vines Diana Sotelo Allison Johnson Evan Dennis Peter Manza Nora D.Volkow Gene-Jack Wang Laboratory of Neuroimaging National Institute on Alcohol Abuse and AlcoholismNational Institutes of Health10 Center DrRm B2L124BethesdaMarylandUnited States

Ketamine,a noncompetitive N-methyl-D-aspartate(NMDA)receptor antagonist,has been exclusively used as an anesthetic in medicine and has led to new insights into the pathophysiology of neuropsychiatric disorders.Clinical studies have shown that low subanesthetic doses of ketamine produce antidepressant effects for individuals with depression.However,its use as a treatment for psychiatric disorders has been limited due to its reinforcing effects and high potential for diversion and misuse.Preclinical studies have focused on understanding the molecular mechanisms underlying ketamine’s antidepressant effects,but a precise mechanism had yet to be elucidated.Here we review different hypotheses for ketamine’s mechanism of action including the direct inhibition and disinhibition of NMDA receptors,aminomethylphosphonic acid receptors(AMPAR)activation,and heightened activation of monoaminergic systems.The proposed mechanisms are not mutually exclusive,and their combined influence may exert the observed structural and functional neural impairments.Long term use of ketamine induces brain structural,functional impairments,and neurodevelopmental effects in both rodents and humans.Its misuse has increased rapidly in the past 20 years and is one of the most common addictive drugs used in Asia.The proposed mechanisms of action and supporting neuroimaging data allow for the development of tools to identify‘biotypes’of ketamine use disorder(KUD)using machine learning approaches,which could inform intervention and treatment.

关键词： Ketamine Neuroimaging N-methyl-D-aspartate dopamine Serotonin Glutamatergic Gamma-aminobutyric acid

Parkinson’s disease and diabetes mellitus: common mechanisms and treatment repurposing

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Neural Regeneration Research 2022年第8期17卷 1652-1658页

作者： Carmen M.Labandeira Arturo Fraga-Bau David Arias Ron Elena Alvarez-Rodriguez Pablo Vicente-Alba Javier Lago-Garma Ana I.Rodriguez-Perez Department of Clinical Neurology Hospital Alvaro CunqueiroUniversity Hospital ComplexVigoSpain Laboratory of Cellular and Molecular Neurobiology of Parkinson’s Disease Research Center for Molecular Medicine and Chronic Diseases(CIMUS)IDISUniversity of Santiago de CompostelaSantiago de CompostelaSpain Department of Clinical Oncology University Hospital ComplexOurenseSpain Department of Endocrinology Hospital MeixoeiroUniversity Hospital ComplexVigoSpain Networking Research Center on Neurodegenerative Diseases(CiberNed) MadridSpain

In the last decade,attention has become greater to the relationship between neurodegeneration and abnormal insulin signaling in the central nervous system,as insulin in the brain is implicated in neuronal survival,plasticity,oxidative stress and neuroinflammation.Diabetes mellitus and Parkinson’s disease are both aging-associated diseases that are turning into epidemics worldwide.Diabetes mellitus and insulin resistance not only increase the possibility of developing Parkinson’s disease but can also determine the prognosis and progression of Parkinsonian symptoms.Today,there are no available curative or disease modifying treatments for Parkinson’s disease,but the role of insulin and antidiabetic medications in neurodegeneration opens a door to treatment repurposing to fight against Parkinson’s disease,both in diabetic and nondiabetic Parkinsonian patients.Furthermore,it is essential to comprehend how a frequent and treatable disease such as diabetes can influence the progression of neurodegeneration in a challenging disease such as Parkinson’s disease.Here,we review the present evidence on the connection between Parkinson’s disease and diabetes and the consequential implications of the existing antidiabetic molecules in the severity and development of Parkinsonism,with a particular focus on glucagon-like peptide-1 receptor agonists.

关键词： antidiabetic diabetes mellitus dopamine exenatide glucagon-like peptide-1 insulin neurodegeneration neuroinflammation Parkinson’s disease repurposing

Astrocytes protect dopaminergic neurons against aminochrome neurotoxicity

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Neural Regeneration Research 2022年第9期17卷 1861-1866页

作者： Juan Segura-Aguilar Bengt Mannervik JoséInzunza Mukesh Varshney Ivan Nalvarte Patricia Muñoz Molecular and Clinical Pharmacology ICBM Faculty of Medicine University of Chile SantiagoChile Department of Biochemistry and Biophysics Arrhenius LaboratoriesStockholm UniversityStockholmSweden Department of Biosciences and Nutrition Karolinska InstitutetStockholmSweden Nucleo de Química y Bioquímica Facultad de Estudios InterdisciplinariosUniversidad MayorSantiagoChile

Astrocytes protect neurons by modulating neuronal function and survival.Astrocytes support neurons in several ways.They provide energy through the astrocyte-neuron lactate shuttle,protect neurons from excitotoxicity,and internalize neuronal lipid droplets to degrade fatty acids for neuronal metabolic and synaptic support,as well as by their high capacity for glutamate uptake and the conversion of glutamate to glutamine.A recent reported astrocyte system for protection of dopamine neurons against the neurotoxic products of dopamine,such as aminochrome and other o-quinones,were generated under neuromelanin synthesis by oxidizing dopamine catechol structure.Astrocytes secrete glutathione transferase M2-2 through exosomes that transport this enzyme into dopaminergic neurons to protect these neurons against aminochrome neurotoxicity.The role of this new astrocyte protective mechanism in Parkinson´s disease is discussed.

关键词： aminochrome astrocytes dopamine dopaminergic neurons exosomes glutathione transferase M2-2 neuroprotection Parkinson’s disease

Molecular mechanisms underlying the neuroprotection of environmental enrichment in Parkinson’s disease

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Neural Regeneration Research 2023年第7期18卷 1450-1456页

作者： Tamara Andrea Alarcón Sarah Martins Presti-Silva Ana Paula Toniato Simões Fabiola Mara Ribeiro Rita Gomes Wanderley Pires Department of Physiological Sciences Health Science CenterUniversidade Federal do Espirito SantoVitoriaBrazil Laboratory of Molecular and Behavioral Neurobiology Health Science CenterUniversidade Federal do Espirito SantoVitoriaBrazil Department of Biochemistry and Immunology Institute o Biological SciencesUniversidade Federal de Minas GeraisAvenida Antônio CarlosBelo HorizonteBrazil

Parkinson’s disease is the most common movement disorder,affecting about 1%of the population over the age of 60 years.Parkinson’s disease is characterized clinically by resting tremor,bradykinesia,rigidity and postural instability,as a result of the progressive loss of nigrostriatal dopaminergic neurons.In addition to this neuronal cell loss,Parkinson’s disease is characterized by the accumulation of intracellular protein aggregates,Lewy bodies and Lewy neurites,composed primarily of the proteinα-synuclein.Although it was first described almost 200 years ago,there are no disease-modifying drugs to treat patients with Parkinson’s disease.In addition to conventional therapies,non-pharmacological treatment strategies are under investigation in patients and animal models of neurodegenerative disorders.Among such strategies,environmental enrichment,comprising physical exercise,cognitive stimulus,and social interactions,has been assessed in preclinical models of Parkinson’s disease.Environmental enrichment can cause structural and functional changes in the brain and promote neurogenesis and dendritic growth by modifying gene expression,enhancing the expression of neurotrophic factors and modulating neurotransmission.In this review article,we focus on the current knowledge about the molecular mechanisms underlying environmental enrichment neuroprotection in Parkinson’s disease,highlighting its influence on the dopaminergic,cholinergic,glutamatergic and GABAergic systems,as well as the involvement of neurotrophic factors.We describe experimental pre-clinical data showing how environmental enrichment can act as a modulator in a neurochemical and behavioral context in different animal models of Parkinson’s disease,highlighting the potential of environmental enrichment as an additional strategy in the management and prevention of this complex disease.

关键词： acetylcholine brain-derived neurotrophic factor dopamine environment enrichment gamma-aminobutyric acid glial cell line-derived neurotrophic factor glutamate molecular mechanisms Parkinson’s disease

Analysis of neurotransmitters validates the importance of the dopaminergic system in autism spectrum disorder

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世界儿科杂志:英文版 2023年第8期19卷 770-781页

作者： Sharmistha Saha Mahasweta Chatterjee Nilanjana Dutta Swagata Sinha Kanchan Mukhopadhyay Manovikas Biomedical Research and Diagnostic Centre Manovikas Kendra482 MadudahPlot I-24Sector-JE.M.BypassKolkataWest Bengal700107India

Background The reasons behind the cardinal symptoms of communication deficits and repetitive,stereotyped behaviors that characterize autism spectrum disorder(ASD)remain unknown.The dopamine(DA)system,which regulates motor activity,goal-directed behaviors,and reward function,is believed to play a crucial role in ASD,although the exact mechanism is still unclear.Investigations have shown an association of the dopamine receptor D4(DRD4)with various neurobehavioral disorders.Methods We analyzed the association between ASD and four DRD4 genetic polymorphisms,5′flanking 120-bp duplication(rs4646984),rs1800955 in the promoter,exon 112 bp duplication(rs4646983),and exon 348 bp repeats.We also examined plasma DA and its metabolite levels,DRD4 mRNA expression,and correlations of the studied polymorphisms with these parameters by case–control comparative analyses.The expression of DA transporter(DAT),which is important in regulating the circulating DA level,was also evaluated.Results A significantly higher occurrence of rs1800955“T/TT”was observed in the probands.ASD traits were affected by rs1800955“T”and the higher repeat alleles of the exon 348 bp repeats,rs4646983 and rs4646984.ASD probands exhibited lower DA and norepinephrine levels together with higher homovanillic acid levels than the control subjects.DAT and DRD4 mRNA expression were down-regulated in the probands,especially in the presence of DAT rs3836790“6R”and rs27072“CC”and DRD4 rs4646984 higher repeat allele and rs1800955“T”.Conclusion This pioneering investigation revealed a positive correlation between genetic variants,hypodopaminergic state,and impairment in socio-emotional and communication reciprocity in Indian subjects with ASD,warranting further in-depth analysis.

关键词： Autism spectrum disorder dopamine Neurotransmitter

Neuro faces of beneficial T cells:essential in brain,impaired in aging and neurological diseases,and activated functionally by neurotransmitters and neuropeptides

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Neural Regeneration Research 2023年第6期18卷 1165-1178页

作者： Mia Levite Faculty of Medicine The Hebrew University of JerusalemCampus Ein KaremJerusalemIsrael Institute of Gene Therapy The Hadassah University Hospital-Ein KaremJerusalemIsrael

T cells are essential for a healthy life,performing continuously:immune surveillance,recognition,protection,activation,suppression,assistance,eradication,secretion,adhesion,migration,homing,communications,and additional tasks.This paper describes five aspects of normal beneficial T cells in the healthy or diseased brain.First,normal beneficial T cells are essential for normal healthy brain functions:cognition,spatial learning,memory,adult neurogenesis,and neuroprotection.T cells decrease secondary neuronal degeneration,increase neuronal survival after central nervous system(CNS) injury,and limit CNS inflammation and damage upon injury and infection.Second,while pathogenic T cells contribute to CNS disorders,recent studies,mostly in animal models,show that specific subpopulations of normal beneficial T cells have protective and regenerative effects in seve ral neuroinflammatory and neurodegenerative diseases.These include M ultiple Sclerosis(MS),Alzheimer’s disease,Parkinson’s disease,Amyotrophic Lateral Sclerosis(ALS),stro ke,CNS trauma,chronic pain,and others.Both T cell-secreted molecules and direct cell-cell contacts deliver T cell neuroprotective,neuro regenerative and immunomodulato ry effects.Third,normal beneficial T cells are abnormal,impaired,and dysfunctional in aging and multiple neurological diseases.Different T cell impairments are evident in aging,brain tumors(mainly Glioblastoma),seve re viral infections(including COVID-19),chro nic stress,major depression,schizophrenia,Parkinson’s disease,Alzheimer’s disease,ALS,MS,stro ke,and other neuro-pathologies.The main detrimental mechanisms that impair T cell function are activation-induced cell death,exhaustion,senescence,and impaired T cell stemness.Fo urth,several physiological neurotransmitters and neuro peptides induce by themselves multiple direct,potent,beneficial,and therapeutically-relevant effects on normal human T cells,via their receptors in T cells.This scientific field is called "Nerve-Driven Immunity".The main neurotransmitters and neuropept

关键词： aging dopamine glutamate nerve-driven immunity neurological diseases neuropeptides neurotransmitters Personalized Adoptive Neuro-Immunotherapy T cells

EFFECT OF INTRAVENTRICULAR INJECTION OF dopamine ON PAIN-EXCITATION AND PAIN-IN HIBITION NEURONS IN CAUDATE NUCLEUS OF RAT

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Chinese Science Bulletin 1989年第16期34卷 1386-1389页

作者：马玉羡谷瑞民孙明智 Department of Physiology Harbin Medical University

Many experiments have shown that the central dopaminergic system is closely related with acupuncture and morphine analgesia. However, there are certain divergences among different authors. Some reported that the central dopaminergic system abates acupuncture and morphine analgesia while others reported that the dopaminergic system coorperates with acupuncture analgesia. The aim of this work is to go further into the relationships of the dopaminergic system in the caudate nucleus (N. Cd.) with pain sensation.

关键词： intraventricle caudate nucleus dopamine