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fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs

Journal of Animal Science and Biotechnology 2017年第3期8卷 574-581页

作者： Daniel S.Ramos Angrimani Marcilio Nichi Joāo Diego A.Losano Cristina F.Lucio Gisele A.Lima Veiga Márcia V.M.Junqueira Franco Camila I.Vannucchi Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of Sāo Paulo Rua Prof. Orlando Marques de Paiva 87 Sāo Paulo 05508-270 Brazil Department of Internal Medicine Division of Nutrition University of Sāo Paulo Av. Bandeirantes 3900 - 14040-040 Ribeirāo Preto. Brazil

Background： During sperm maturation, there is a reorganization of fatty acids from plasmatic membrane of the spermatozoa, which allows higher membrane integrity and acquisition of sperm motility. However, the fatty acid profile during sperm maturation remains unclear in dogs. Thus, the aim of this study was to identify the fatty acids from the epididymal spermatozoa and plasma during the sperm maturation, and observed changes in the motility and plasmatic membrane parameters. Twenty one adult dogs were used, subsequently to bilateral orchiectomy and epididymal storage, sperm samples were collected from the different segments of the epididymis. Samples were evaluated for conventional microscopy, computer-assisted motility analysis, sperm plasma membrane permeability and the fatty acid analysis（lipids were extracted, transmethylated and analyzed by chromatography）.Results： Caput and corpus sperm showed lower values for the motility variables evaluated and plasmatic membrane integrity, indicating different levels of the fatty acids organization. Saturated, monounsaturated and polyunsaturated fatty acids were in higher concentrations in the spermatozoa from epididymis cauda. Highlighting the presence of caprylic, stearic and docosahexaenoic ***： These findings demonstrate the influence of the fatty acid profile during sperm maturation, assigning physical and chemical changes in sperm cells, essential for fertilization.

关键词： dogs epididymis fatty acids sperm maturation

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