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A versatile biosensing platform coupling CRISPR-Cas12a and aptamers for detection of diverse analytes

Science Bulletin 2021年第1期66卷 69-77,M0004页

作者： Xiangxiang Zhao Shanshan Li Guang Liu Zhong Wang Zhiheng Yang Quanwei Zhang Mindong Liang Jiakun Liu Zilong Li Yaojun Tong Guoliang Zhu Xinye Wang Lan Jiang Weishan Wang Gao-Yi Tan Lixin Zhang State Key Laboratory of Bioreactor Engineering and School of BiotechnologyEast China University of Science and Technology(ECUST)Shanghai 200237China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Department of Urology Sixth People’s Hospital South Campus Affiliated to Shanghai Jiao Tong UniversityShanghai 201499China Shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China The Novo Nordisk Foundation Center for Biosustainability Technical University of DenmarkKgs Lyngby 2800Denmark University of Chinese Academy of Sciences Beijing 100049China

Rapid and sensitive detection of various analytes is in high *** from its application in genome editing,CRISPR-Cas also shows promises in nucleic acid detection *** further exploit the potential of CRISPR-Cas for detection of diverse analytes,we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR-Cas12 *** demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background,i.e.,the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 lmol/L,respectively,highlighting the advantages of simplicity,sensitivity,short detection time,and low cost compared with the state-of-the-art biosensing ***,this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes.

关键词： CRISPR–Cas12a Aptamer Biosensing platform diverse analyte Alpha fetoprotein detection Cocaine detection

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