检索结果-南通市图书馆

Selection of reference genes for RT-qPCR analysis of Phenacoccus solenopsis(Hemiptera: Pseudococcidae) sex-dimorphic development

引用

Journal of Integrative Agriculture 2019年第4期18卷 854-864页

作者： ZHENG Lu-ying ZHANG Zhi-jun ZHANG Jin-ming LI Xiao-wei HUANG Jun LIN Wen-cai LI Wei-di LI Chuan-ren LU Yao-bin College of Agriculture Yangtze University Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences

Mealybugs, such as Phenacoccus solenopsis, are highly sexually dimorphic. Winged adult males present such remarkable morphological differences from females that, to the untrained eye, conspecific adults of both sexes of P. solenopsis may be considered as two different insect species. A method to investigate sex-dimorphic mechanisms is by evaluating gene expression using RT-qPCR. However, the accuracy and consistency of this technique depend on the reference gene(s) selected. In this study, we analyzed the expression of 10 candidate reference genes in male and female P. solenopsis at different development stages, using common algorithms including the ?Ct method, NormFinder, geNorm, BestKeeper, and a web-based analysis tool, RefFinder. The results showed that EF1-β, RP-L32 and RP-18 S were selected as the most stable genes by both the ?Ct method and NormFinder; TUB-α was the most stable gene identified by BestKeeper; and RP-L40 and RP-L32 were the most stable genes ranked by geNorm. RefFinder, a comprehensive analysis software, ranked the ten genes and determined EF1-β and RP-L32 as the most suitable reference genes for the various developmental stages in male and female P. solenopsis. Furthermore, the two most suitable reference genes were validated by examining expression of the juvenile hormone acid O-methytransferase(JHAMT) gene. Results of the validation portion of the study showed that JHAMT expression was sex-biased towards males and exhibited a dynamic and classic expression pattern among the P. solenopsis developmental stages. The results can help further our knowledge on the molecular mechanisms underlying sexual dimorphic development in P. solenopsis.

关键词： Phenacoccus solenopsis RT-qPCR sexual dimorphic development reference gene gene stability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cellular Prion Protein and Sexual dimorphic Areas in Rodents. Correlates with Alzheimer Disease

引用

Neuroscience & Medicine 2011年第4期2卷 384-391页

作者： Mar Cuadrado-Tejedor Ana Irujo Beatriz Paternain María Jesús Madrid José Luis Velayos 不详

The cellular prion protein (PrPC) expression was analyzed by western-blot in the rat, in two different dimorphic brain areas such as the anterior hypothalamic and the preoptic areas. In both cases, the PrPC expression was increased in males, implying a sexual dimorphism for the PrPC protein. The study was also made in other two brain areas, frontal cortex and hyppocampus (a clearly dimorphic area);in this case, mice of different ages of both sexes were used. In both brain areas analyzed, although the PrPC expression was increased with age until the adult age (38 weeks), it was decreased in aged animals (56 weeks) in both sexes. The PrPC expression in mouse hippocampus was predominant in males in comparison to females. Moreover, the non-glycosylated band was increased with age and this increase was parallel with the increase observed for the glycolsylated band. The non-glycosylated band increases more in aged females. Altogether, these data suggest that PrP in rodents, in the brain areas analyzed, has a dimorphism role. As we discuss in the present study and in relation to previous studies of our group these data could be extrapolated to humans (specially in Alzhemer disease cases).

关键词： Prion Protein (PrPc) dimorphic Alzheimer Hippocampus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：