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diffusion-weighted 7.0T Magnetic Resonance imaging in Assessment of intervertebral disc degeneration in Rats

Chinese Medical Journal 2018年第1期131卷 63-68页

作者： Long-Yang Li Xiao-Lin Wu Richard J Roman Fan Fan Chen-Sheng Qiu Bo-Hua Chen Department of Pharmacology and Toxicology University of Mississippi Medical Center MS 39216 USA Department of Orthopedics The Affiliated Hospital of Qingdao University Qingdao Shandong 266003 China

Background： lntervertebral disc degeneration （IDD） is a major cause of disc protrusion, likely to be associated with decrease of water content. This research aimed to evaluate IDD by diffusion-weighted imaging （DWI） with a 7.0 Tesla iT） magnetic resonance imaging （M RI） machine. Methods： A total of 24 healthy Sprague-Dawley rats were randomly selected and divided into four groups （A, B, C, and D）, each consisting of 3 male and 3 female rats （28, 42, 56, and 70 days old, respectively）. All the rats were imaged with a 7.0T MRI, producing T2WI, T1WI, and functional DWI sequences. Data were collected and apparent diffusion coefficient （ADC） charts were constructed. Nucleus pulposus and annulus fibrosus regions were identified, several regions of interest were chosen, and their ADC values were obtained. After imaging, rats were sacrificed and their intervertebral discs （ L I-L6） were dissected, yielding a total of 144 discs. Protein was extracted for the purpose of Western blotting. Comparison among multiple samples used one-way analysis of variance and least significant difference methods. Results： 7.0T MRI revealed evident decrease in signal intensity within intervertebral discs of Sprague-Dawley rats with age. lntervertebral disc A DC values significantly decreased from Group A （0.00154 ± 0.00008） to Group D （0.00107 ± 0.00007： P 〈 0.01 ）; nucleus pulposus ADC values significantly decreased from Group A （0.00164± 0.00005） to Group D （0.00140± 0.00007; P 〈 0.01 ） and annulus fibrosus ADC values significantly decreased from Group A （0.00129 ± 0.00014） to Group D （0.00082 ± 0.00012; P 〈 0.01）, Meanwhile, it also revealed evident decrease from high spinal level to low spinal level： nucleus pulposus ADC values in GroupA significantly decreased from L1/L2 （0.00163 ± 0.00006） to L6/S1 （0.00139 ± 0.00004, P 〈 0.01 ）. While annul us fibrosus ADC values did not di tier significantly between levels in Group A （P 〉 0.05）. Western blotting showed that aggrecan content ofinterverte

关键词： Apparent diffusion Coefficient diffusion-weighted imaging: intervertebral disc degeneration Magnetic Resonance imaging

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