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Use of lectin microarray to differentiate gastric cancer from gastric ulcer

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World Journal of Gastroenterology 2014年第18期20卷 5474-5482页

作者： Wei-Li Huang Yang-Guang Li Yong-Chen Lv Xiao-Hui Guan Hui-Fan Ji Bao-Rong Chi Department of Hepatology the First Clinic Hospital of Jilin University Department of Gastroenterology Affiliated Hospital of Beihua University Laboratory of Medicinal Chemical Biology College of Life SciencesNankai University Department of General Surgery the Central Hospital of Jilin City

AIM: To investigate the feasibility of lectin microarray for differentiating gastric cancer from gastric ulcer. METHODS: Twenty cases of human gastric cancer tissue and 20 cases of human gastric ulcer tissue were collected and processed. Protein was extracted from the frozen tissues and stored. The lectins were dissolved in buffer, and the sugar-binding specificities of lectins and the layout of the lectin microarray were summarized. The median of the effective data points for each lectin was globally normalized to the sum of medians of all effective data points for each lectin in one block. Formalin-fixed paraffin-embedded gastric cancer tissues and their corresponding gastric ulcer tissues were subjected to Ag retrieval. Biotinylated lectin was used as the primary antibody and HRP-streptavidin as the secondary antibody. The glycopatterns of glycoprotein in gastric cancer and gastric ulcer specimens were determined by lectin microarray, and then validated by lectin histochemistry. Data are presented as mean +/- SD for the indicated number of independent experiments. RESULTS: The glycosylation level of gastric cancer was significantly higher than that in ulcer. In gastric cancer, most of the lectin binders showed positive signals and the intensity of the signals was stronger, whereas the opposite was the case for ulcers. Significant differences in the pathological score of the two lectins were apparent between ulcer and gastric cancer tissues using the same lectin. For MPL and VVA, all types of gastric cancer detected showed stronger staining and a higher positive rate in comparison with ulcer, especially in the case of signet ring cell carcinoma and intra-mucosal carcinoma. GalNAc bound to MPL showed a significant increase. A statistically significant association between MPL and gastric cancer was observed. As with MPL, there were significant differences in VVA staining between gastric cancer and ulcer. CONCLUSION: Lectin microarray can differentiate the different

关键词： Gastric cancer Gastric ulcer Lectin microarray Lectin histochemistry differentiate

Magnetic Inductive Phase Shift: A New Method to differentiate Hemorrhagic Stroke from Ischemic Stroke on Rabbit

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Chinese Journal of Biomedical Engineering(English Edition) 2017年第2期26卷 81-81页

The major therapy for ischemic stroke is thrombolytic treatment, but severe consequences occurwhen this method is used to treat hemorrhagic stroke. Currently, computed tomography and magneticresonance imaging are used... 详细信息

关键词： Magnetic Inductive Phase Shift: A New Method differentiate Hemorrhagic Stroke Ischemic Stroke on Rabbit

Ultrastructure and electrophysiology of astrocytes differentiated from adult adipose-derived stromal cells

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Chinese Medical Journal 2011年第17期124卷 2656-2660页

作者： OU Ya YUAN Xiao-dong CAI Ya-nan LU Yan-hui Department of Neurology Kailuan General Hospital Hebei United University Tangshan Hebei 063000 China

Background Adipose-derived stromal cell （ADSC） differentiation into neural cells in vitro is becoming widely studied. However, there are few reports on astrocytes following differentiation, and particularly on maturation and electrophysiology. In this study, we used various methods to determine ADSC-derived astrocyte maturity. Methods Chemical induction with isobutylmethylxanthine （IBMX） was used to differentiate adult ADSCs into astrocytes followed by hematoxylin-eosin （HE） staining to observe morphology and transmission electron microscopy for cellular ultrastructure assessment. Immunofluorescence was used to detect expression of neural stem cell marker nestin as well as glial markers glial fibrillary acidic protein （GFAP） and S-100. In addition, we measured membrane potentials in bis-（1,3-dibarbituric acid） trimethine oxanol-labeled ADSCs and astrocytes by stimulation with a high potassium solution under an inverted fluorescence microscope. Finally, cell cycle distribution was detected by flow cytometry. Results Typical astrocyte morphology was shown by HE staining after 48-hour differentiation. Glial fibril was observed with transmission electron microscopy. GFAP and S-100 were not expressed in the control group, but were expressed within 24-hour differentiation and reached a maximum at day 14 with no change up to day 28. Nestin was weakly expressed in control cells and also reached a maximum at day 14 with the percentage of positive cells constant until day 21 followed by a decrease. differentiated cell membrane potentials after stimulation with potassium were slightly increased, and then gradually declined over time. There was no significant membrane potential change in the control group. Flow cytometry showed that the percentage of cells in G0/G1 phase was 93% and only 5% in S phase. Conclusion ADSCs were differentiated into mature astrocytes with typical characteristics including morphology, ultrastructure, marker protein expression, mature potassium channel

关键词： adult adipose-derived stromal cells differentiate astrocytes ultrastructure membrane potential

Single cell tells the developmental story

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Science Bulletin 2016年第17期61卷 1355-1357页

作者： Zhengyan Zhan Xin Xu Yu V.Fu State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of Sciences

Stem cells are undifferentiated cells that retain the ability to self-renew and differentiate into specialized cells,offering a promising resource to generate requisite cell types in regenerative medicine[1,2].Haematopoietic stem cells(HSCs)can differentiate into entire mature blood lineages,sitting at the apex of the haematopoietic hierachy and playing a key role in the long-term maintenance and stabilization of mammalian blood system functions[3,4].HSCs are mostly found in embryo and bone marrow,but also with small amounts in peripheral ***

关键词： maintenance differentiate specialized stabilization embryo mature story retain offering developmental

Differentiation of Mesenchymal Stem Cells Into Dopaminergic Neuron-like Cells in vitro

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Biomedical and Environmental Sciences 2005年第1期18卷 36-42页

作者： L1GUO FEIYIN HONG-QIMENG LINGLING TA-NAHU-HE PENGLI CHUN-XIAZHANG SHUNYU DE-SHENGDUAN HONG-XUEFAN DepartmentofToxicology SchoolofPublicHealthJilinUniversityChangchunJilin130021China DepartmentofOrthopaedics China-JapanUnionHospitalJilinUniversityChangchunJilin130031China BeijingInstituteofGeriatrics XuanwuHospitalofCapitalUniversityofMedicalSciencesBeijing100053China

To explore the way to induce mesenchymal stem cells (MSCs) to differentiate into dopaminergic neurons in vitro. Methods MSCs were obtained from rat bone marrow, cultured and passaged. MSCs used in this experiment had multipotency, which was indirectly proved by being induced to differentiate into chondrocytes and adipocytes. MSCs were cultured in medium containing 0.5 mmol/L IBMX for 2 days. Then the medium was replaced with induction medium, which contained GDNF, IL-1β, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments. The surface markers of the differentiated neurons, such as NSE, nestin, MAP-2a, b and TH were detected by immunocytochemistry and Western blot after MSCs were cultured in induction medium for 7 days and 15 days. Results MSCs differentiated into neural progenitors and expressed nestin after MSCs were incubated with medium containing IBMX for 2 d. After the medium was replaced with induction medium containing many inducing agents, MSCs differentiated into neuron-like cells and dopaminergic neuron-like cells and expressed NSE, MAP-2a, b and TH. The percentage of NSE-positive cells, MAP-2a, b-positive cells and TH-positive cells was 30.032±2.489%, 41.580±5.101% and 34.958±5.534%, respectively after MSCs were induced in medium containing GDNF, IL-1β, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments for 15 days. Conclusion MSCs can differentiate into dopaminergic neuron-like cells and are a new cell source for the treatment of neurodegeneration diseases and have a great potential for wide application

关键词： Mesenchymal stem cells (MSCs) Dopaminergic neuron-like cells (DA neuron-like cells) differentiate

Microgravity may help future organ/tissue manufacture

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Science China(Life Sciences) 2016年第8期59卷 850-853页

作者： Jin Han Jianwu Dai State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101China

Stem cells are a group of multipotent or pluripotent cells which could differentiate into specialized cells or could self-renew to duplicate themselves. During the body devel- oping period, stem cells differentiated into specialized cells to build the whole body. In the adult organisms, stem cells live in seclusion and are ready to repair the injuries of tis- sues. Within one tissue, stem cells could differentiate in to all types of somatic cells of the tissue, for example,

关键词： manufacture specialized pluripotent differentiate duplicate renew repair themselves ready injuries

Mechanobiology:A new frontier for human pluripotent stem cells

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医用生物力学 2013年第S1期28卷 17-19页

作者： Jianping Fu Yubing Sun Koh Meng Aw Yong Integrated Biosystems and Biomechanics Laboratory Department of Mechanical EngineeringDepartment of Biomedical EngineeringUniversity of Michigan

Our understanding of the molecular and cellular mechanisms that control self-renewal,pluripotency and differentiation of human pluripotent stem cells(hPSCs)and our progress toward harnessing the regenerative potential of these cells to treat human diseases are advancing at a rapid *** pluripotent stem cells(hPSCs)include human embryonic stem cells(hESCs)and human induced pluripotent stem cells(hiPSCs).Their unique capacity for indefinite self-renewal(unlimited proliferation)in vitro coupled with their ability to differentiate into almost any cell type present in the adult body(pluripotency)provide a potentially

关键词： pluripotent embryonic differentiate renewal potentially frontier regenerative advancing toward innovative

In vitro Differentiation of Adipose-Derived Stem Cells into Hair Cell-Like Cells in Guinea Pigs

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Journal of Otology 2007年第2期2卷 97-101页

作者： WANG Ying1, DONG Min-min1, YANG Hai-bo2, GAO Song-tao3 1 Department of Otolaryngology, the First Affiliated Hospital of Zhengzhou University, China 2 Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, China 3 Department of orthopaedics, Xiangya Hospital of Central South University, China Department of Otolaryngology the First Affiliated Hospital of Zhengzhou University China Department of Cardiology The First Affiliated Hospital of Zhengzhou University China Department of orthopaedics Xiangya Hospital of Central South University China

Objective Adipose-derived stem cells(ADSCs) are suggested to possess a highly plastic ability to differentiate into several specific cell types in addition to adipocyte lineages, including germ layer tissue-specific cell lineages such as chondrocyte, myocyte, neuronal, and osteoblast lineages. The aim of this study is to establish an in vitro culture technique for ADSCs in an adult guinea pig model that facilitate their differentiation into hair cell-like cells. Materials and Methods Cells from inguinal fat pads in adult guinea pigs were cultured with β-mercaptoethanol, RA, Forskorin, Heregulin, bFGF, BDNF and EGF. Cellular differentiation was examined using immunocytochemistry techniques. Results The ADSCs demonstrated hair cell immunophenotypes with expression of epitopes of the hair cell marker protein myosin Ⅶa. Conclusion ADSCs from adult guinea pig adipose tissue can differentiate into hair cell-like cells when cultured in vitro. ADSCs may serve as seed cells for tissue engineering.

关键词： adipose-derived stem cell hair cell differentiate guinea pig

Research progress in Human Adipose Tissue-derived Stromal Cells

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中国输血杂志 2010年第S1期23卷 103-104页

作者： Sun Jianhua,Liu Jie,Zhao Guoqing,HouLing,Ding JuanThe Blood center of Heilongjiang 150056

Objectives this article aim to review the research progress,applications and existing problems of human ADSCs in tissue engineering and cell therapy by retrieving relevant publications. Methods Retrieval Strategy:PUBMED and CNKI database were undertaken to identify the relevant articles published from 1960 to 2009 with the key words of "Human adipose tissue-derived stromal cells,isolation,differentiation,immune phenotype,molecular expression,

关键词： adipose isolation Retrieval publications phenotype differentiate osteoblast fetal Criterion bovine