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dieckol isolated from Eisenia bicyclis extract suppresses RANKL-induced osteoclastogenesis in murine RAW 264.7 cells

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Asian Pacific Journal of Tropical Biomedicine 2022年第6期12卷 262-269页

作者： Su-Hyeon Cho Tae-Hyung Kwon Hoibin Jeong Jin Sook Kim Song-Rae Kim Myeong Seon Jeong SeonJu Park Miri Choi Jung-Hee Woo Juhee Ahn Kil-Nam Kim Chuncheon Center Korea Basic Science InstituteChuncheon 24341Republic of Korea Department of Medical Biomaterials Engineering College of Biomedical SciencesKangwon National UniversityChuncheon 24341Republic of Korea Department of Research and Development Chuncheon Bio-industry FoundationChuncheonRepublic of Korea Marine Industry Research Institute for East se rim Uljin-gunGyeongsangbuk-doRepublic of Korea Department of Bio-analysis Science University of Science&TechnologyDaejeon34113Republic of Korea

Objective:To demonstrate the effect of dieckol from Eisenia bicyclis on osteoclastogenesis using RAW 264.7 ***:Murine macrophage RAW 264.7 cells were subjected to dieckol treatment,followed by treatment with receptor activator of nuclear factor kappa-B ligand(RANKL)to induce ***-resistant acid phosphatase(TRAP)activity was examined using a TRAP activity *** blotting analysis was conducted to examine the level of osteoclast-related factors,including TRAP and calcitonin receptor(CTR),transcriptional factors,including c-Fos,c-Jun,and nuclear factor of activated T cells cytoplasmic 1(NFATc1),nuclear factor kappa-B(NF-κB),extracellular signal-regulated kinase(ERK),and c-Jun N-terminal kinase(JNK).Immunofluorescence staining was conducted to examine the expression of c-Fos,c-Jun,and ***:Among the four phlorotannin compounds present in Eisenia bicyclis,dieckol significantly hindered osteoclast differentiation and expression of RANKL-induced TRAP and *** addition,dieckol downregulated the expression levels of c-Fos,c-Jun,NFATc1,ERK,and JNK,and suppressed NF-κB ***:dieckol can suppress RANKL-induced ***,it has therapeutic potential in treating osteoclastogenesis-associated diseases.

关键词： Eisenia bicyclis dieckol Osteoclasts ERK JNK NF-κB RANKL TRAP Calcitonin receptor NFATc1 RAW 264.7 cell

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Evaluation for Antioxidative Properties of Phlorotannins Isolated from the Brown Alga <i>Eisenia bicyclis</i>, by the H-ORAC Method

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Food and Nutrition Sciences 2013年第8期4卷 78-82页

作者： Yohei Fujii Reiji Tanaka Hideo Miyake Yutaka Tamaru Mitsuyoshi Ueda Toshiyuki Shibata Japan Science and Technology Agency CREST Tokyo Japan Division of Applied Life Sciences Graduate School of Agriculture Kyoto University Kyoto Japan Major of Life Sciences Graduate School of Bioresources Mie University Mie Japan Major of Life Sciences Graduate School of Bioresources Mie University Mie Japan Japan Science and Technology Agency CREST Tokyo Japan

The antioxidative properties of phlorotannins isolated from the brown alga Eisenia bicyclis were measured using the H-ORAC (Hydrophilic Oxygen Radical Absorbance Capacity) method. The ORAC values of phloroglucinol and it’s oligomers: eckol, fucofuroeckol A, phlorofucofuroeckol A, dieckol, 8,8’-bieckol, were 2.57 ± 0.14, 4.97 ± 0.36, 9.82 ± 0.70, 8.97 ± 0.89, 10.22 ± 0.85, 8.62 ± 0.92 μmol Trolox equivalent/μmol, respectively. With the exception of eckol, the ORAC values of tested phlorotannins were higher than those of the well-known antioxidants (epigallocatechin gallate, resveratrol and L-ascorbic acid) used as positive controls. As a result of comparing with known ORAC values, it was found that the dieckol and fucofuroeckol A had stronger antioxidant activity than representative polyphenols (e.g., kaempferol, quercetin, myricetin and chlorogenic acid) derived from terrestrial plants.

关键词： Antioxidants Brown Alga dieckol Eisenia bicyclis Fucofuroeckol A H-ORAC Assay Phlorotannins

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