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Development of diagnostic markers for a wheat leaf rust resistance gene Lr42 using RNA-sequencing

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The Crop Journal 2021年第6期9卷 1357-1366页

作者： Yang Liu Hui Chen Chunxin Li Lirong Zhang Mingqin Shao Yuhui Pang Xiangyang Xu Guihua Bai Institute of South Subtropical Crops Chinese Academy of Tropical Agricultural SciencesZhanjiang 524013GuangdongChina Department of Agronomy Kansas State UniversityManhattanKS 66506USA Henan Academy of Agricultural Science Zhengzhou 450002HenanChina College of Plant Protection Hebei Agricultural UniversityBaoding 071000HebeiChina College of Agriculture Henan University of Science and TechnologyLuoyang 471023HenanChina USDA-ARS Wheat Peanutand Other Field Crop Research UnitStillwaterOK 74075USA USDA-ARS Hard Winter Wheat Genetic Research UnitManhattanKS 66506USA

Wheat leaf rust is a prevalent foliar disease in wheat worldwide. Growing resistant cultivars is an effective strategy to minimize the impact of leaf rust on yield and grain quality. Lr42 is a leaf rust resistance gene identified from Aegilops tauschii and is still effective against current predominant leaf rust races in the United States and many other countries. In this study, we developed diagnostic DNA markers for Lr42 using the sequence polymorphisms of a differentially expressed gene(TaRPM1) encoding a putative NBARC protein in the Lr42 candidate region identified by RNA-sequencing of two near-isogenic lines contrasting in Lr42 alleles. markers were designed based on a deletion mutation and a single nucleotide polymorphism(SNP) in the gene. Haplotype analyses of the newly developed markers in the three diversity panels demonstrated that they are diagnostic for Lr42, and superior to previously used markers in selection accuracy. These markers have the advantages of low cost and easy assay, and they are suitable for marker-assisted selection in breeding programs with either high-or low-throughput marker screening facilities.

关键词： Aegilops tauschii Triticum tauschii Lr42 KASP diagnostic marker

Novel trends in celiac disease

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Cellular & Molecular Immunology 2011年第2期8卷 121-125页

作者： Torsten Matthias Sandra Neidhofer Sascha Pfeiffer Kai Prager Sandra Reuter M Eric Gershwin AESKU.KIPP Institute WendelsheimGermany AESKU.diagnostics GmbH&Co.KG WendelsheimGermany Division of Rheumatology Allergyand Clinical ImmunologyUniversity of CaliforniaDavisCAUSA

Celiac disease(CD)is one of the most common food intolerances in developed *** affects genetically susceptible individuals and has severe consequences if it remains undiagnosed.A disease known for more than a century,it is still the focus for experts from various fields of research and ***,pathologists,immunologists,food engineers and dieticians share their knowledge and expertise to improve the conditions of CD *** new insights in the pathomechanism of gluten processing and antigen presentation in CD,it was possible to improve the diagnostic antigen mimicking the primary epitope in *** celiac neo-epitopes are comprised of a complex of gliadin peptides crosslinked with transglutaminase(tTg).They are an early diagnostic marker for CD which occurs up to 6 months earlier than classical markers known to miss a certain amount of CD patients.

关键词： celiac disease tissue transglutaminase celiac neo-epitope gluten diagnostic marker

diagnostic role of serum interleukin-18 in gastric cancer patients

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World Journal of Gastroenterology 2006年第28期12卷 4473-4477页

作者： Duangporn Thong-Ngam Pisit Tangkijvanich Rungsun Lerknimitr Varocha Mahachai Apiradee Theamboonlers Yong Poovorawan Department of Physiology Faculty of MedicineChulalongkorn UniversityBangkok10330Thailand Department of Biochemistry Faculty of MedicineChulalongkorn UniversityBangkok10330Thailand Department of Medicine Faculty of MedicineChulalongkorn UniversityBangkok10330Thailand Viral Hepatitis Research Unit Department of PediatricsFaculty of MedicineChulalongkorn UniversityBangkok10330Thailand

AIM： To determine the current status in various aspects of gastric cancer patients and to find out the clinical correlation with prognostic role of serum interleukins in Thai patients. METHODS： Sixty-eight patients were enrolled in this study at King Chulalongkorn Memorial Hospital during April 2003 to May 2005. Gastric cancer was histologically proven in 51 patients and gastric ulcer in 17 patients. Serum IL-6, IL-10, IL-12, and IL-18 levels were measured by enzyme-linked immunosorbent assay （ELISA）. RESULTS： There were 26 males （55.32%） and 21 females （44.68%） with their age ranging from 33 to 85 years （mean age 64.49 ± 13.83 years）. The common presentations were weight loss （41.2%）, dyspepsia （39.2%）, and upper gastrointestinal bleeding （15.7%）. A total of 35.3% gastric cancer patients and 6.3% of gastric ulcer patients were smokers （P = 0.029）. Moreover, 32.4% of gastric cancer patients and 6.3% of gastric ulcer patients were alcoholic drinkers （P = 0.044）. Lesion location was pyloric-antrum in 39.4%, gastric body in 39.4%, upper stomach in 12.2% and entire stomach in 6.1% of the patients. H pylori infection was detected in 44.4%. The poorly-differentiated adenocarcinoma was the most common pathologic finding （60.7%）. Surgical treatment was performed in 44.1% patients （total gastrectomy in 5.9%, subtotal gastrectomy in 32.4% and palliative bypass surgery in 5.9%）. Systemic chemotherapy was given as an adjuvant therapy in 8.8% patients. Carcinomatosis peritoneii were found in 18.8% patients. The mean survival time was 13.03 ± 9.75 mo. The IL-18 level in gastric cancer patient group （58.54 ± 43.96 pg/mL） was significantly higher than that in gastric ulcer patient group （30.84± 11.18 pg/mL）（P = 0.0001）（95% CI was 42.20, 13.19）. The cut point of IL-18 for diagnosis of gastric cancer was 40 pg/mL, the positive predictive value was 92.31%. The IL-6 level in gastric cancer patients with distant metastasis （20.21 ±9.37 pg/mL） was significantly higher than that in thos

关键词： Interleukin Gastric cancer diagnostic marker

Potential Role and Clinical Value of PPP2CA in Hepatocellular Carcinoma

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Journal of Clinical and Translational Hepatology 2021年第5期9卷 661-671页

作者： Cheng-Lei Yang Xue Qiu Jin-Yan Lin Xiao-Yu Chen Yu-Mei Zhang Xiao-Yin Hu Jian-Hong Zhong Shen Tang Xi-Yi Li Bang-De Xiang Zhi-Ming Zhang Department of Hepatobiliary Surgery Guangxi Medical University Cancer HospitalNanningGuangxiChina The First Clinical Medical School Guangxi Medical UniversityNanningGuangxiChina Department of Pathology Guangxi Medical University Cancer HospitalNanningGuangxiChina Department of Medical Oncology Affiliated Tumor Hospital of Guangxi Medical UniversityNanningGuangxiChina School of Basic Medical Sciences Guangxi Medical UniversityNanningGuangxiChina School of Public Health Guangxi Medical UniversityNanningGuangxiChina

Background and Aims:Protein phosphatase 2A(PP2A)is associated with many *** study aimed to clarify whether PPP2CA,which encodes the alpha isoform of the catalytic subunit of PP2A,plays a role in hepatocellular carcinoma(HCC)and to identify the potential underlying molecular ***:Based on bioinformatics,public databases and our in-house RNA-Seq database,we analyzed the clinical value and molecular mechanism of PPP2CA in ***:Data were analyzed from 2,545 patients with HCC and 1,993 controls without HCC indexed in The Cancer Genome Atlas database,the Gene Expression Omnibus database and our in-house RNA-Seq ***2CA expression was significantly higher in HCC tissue than in non-cancerous tissues(standardized mean difference:0.69,95%confidence interval[CI]:0.50–0.89).PPP2CA expression was able to differentiate HCC from non-HCC,with an area under the summary receiver operator characteristic curve of 0.79(95%CI:0.75–0.83).Immunohistochemistry of tissue sections confirmed that PPP2CA protein was up-regulated in HCC *** PPP2CA expression in HCC patients was associated with shorter overall,progression-free and disease-free *** molecular pathways through which PPP2CA may be involved in HCC were determined using miRWalk 2.0 as well as analysis of Gene Ontology categories,Kyoto Encyclopedia of Genes and Genomes pathways,and protein-protein interaction ***:PPP2CA is up-regulated in HCC and higher expression correlates with worse ***2CA shows potential as a diagnostic marker for *** studies should examine whether PPP2CA contributes to HCC through the candidate microRNAs,pathways and hub genes identified in this study.

关键词： Hepatocellular carcinoma PPP2CA Molecular mechanism diagnostic marker

Clinical and prognostic significance of expression of phosphoglycerate mutase family member 5 and Parkin in advanced colorectal cancer

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World Journal of Clinical Cases 2022年第14期10卷 4368-4379页

作者： Can Wu Ming-Liang Feng Tai-Wei Jiao Ming-Jun Sun Department of Endoscope The First Hospital Affiliated to China Medical UniversityShenyang 110001Liaoning ProvinceChina

BACKGROUND Drugs targeting mitochondria can induce mitophagy and restrain proliferation in colorectal cancer(CRC)*** mutase family member 5(PGAM5)activates serine/threonine PTEN-induced putative kinase 1/Parkin pathway-mediated ***,there are few studies on the clinical and prognostic significance of expression of PGAM5 protein and mitophagy-related protein Parkin in *** To assess the clinical significance of PGAM5 and Parkin proteins,as biomarkers for diagnosis and prognosis of CRC,by studying their expression in advanced CRC tissues and their association with clinicopathological *** The expression of PGAM5 and Parkin in CRC tissues from 100 patients was determined by *** case was evaluated by using a combined scoring method based on signal intensity staining(scored 0-3)and the proportion of positively stained cancer cells(scored 0-4).The final staining score was calculated as the intensity score multiplied by the proportion *** were categorized as either high or low expression according to the Youden index,and the association between the expression of PGAM5 or Parkin and clinicopathological factors was ***,we employed western blot to measure PGAM5 and Parkin protein expression in six matched pairs of CRC and adjacent non-tumor *** Immunohistochemical and western blot findings showed that both PGAM5 and Parkin protein expression in tumor tissues was significantly higher than that in the adjacent tissues:PGAM5 and Parkin were mainly expressed in the cytoplasm of colonic epithelial ***5 and Parkin protein levels were significantly positively correlated in advanced CRC ***,reduced Parkin protein expression was an independent prognostic factor for overall survival and progression-free survival in CRC patients as evinced by multivariate *** The expression of PGAM5 protein and mitophagy-related protein Parkin has d

关键词： Phosphoglycerate mutase family member 5 Parkin,Mitophagy Colorectal cancer diagnostic marker Cancer prognosis

Role of the circulatory interleukin-6 in the pathogenesis of gliomas:A systematic review

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World Journal of Methodology 2022年第5期12卷 428-437页

作者： Manish Singh Alok Raghav Kirti Amresh Gautam Department of Neurosurgery GSVM Medical CollegeKanpur 208001India Department of Basic and Applied Science GD Goenka UniversityGurugram 122103HaryanaIndia

BACKGROUND Glioma is the most common primary tumor in the brain originating from glial *** spite of extensive research,the overall survival rate is not enhanced.A number of published articles observed differentially circulating levels of cytokines in ***-6(IL-6)protein coded by IL-6 gene is regulated by the immune system and it has been found to have a significant role in progression and apoptosis resistance of *** To review the role of circulatory IL-6 in the development and progression of glioma and its utility as a *** Preferred Reporting Items for Systematic Reviews and Meta-analysis(PRISMA)guidelines were applied to filter the relevant studies based on inclusion and exclusion *** used a combination of keywords and the Reference Citation Analysis(RCA)tool to search the potential studies and performed data extraction from selected *** The published results were inconsistent;however,most studies showed a significantly higher IL-6 level in glioma cases as compared to *** IL-6 level among the different grades of glioma showed a higher level with low-grade gliomas and lower level with high-grade *** IL-6 level significantly differed between cases and controls,and among different cancer stages,which shows its potential as a diagnostic and prognostic marker.

关键词： Gliomas Interleukin-6 Circulatory markers diagnostic marker Prognostic marker