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Effects of Transport,Storage Temperature and Time on Parthenogenetically developmental competence of Sheep Oocyte

Animal Husbandry and Feed Science 2014年第3期6卷 138-140页

作者： Qiao Limin Qiao Fuqiang Guo Tong Yao Hua Zhang Jinghe Xiao Xishan Hou Yinxu Guan Weijun Department of Husbandry and Veterinary Beijing Vocational College of Agriculture Group of Science Technology and Industry Beijing University of Agriculture Institute of Animal Science and Veterinary Medicine Beijing University of Agriculture Institute of Animal Science( IAS) Chinese Academy of Agricultural Science( CAAS)

This study was to investigate the effects of transport, storage temperature and time on parthenogenetical developmental competence of sheep oocyte. The ovaries were collected and randomly immersed in physiological saline at （ 10 - 15 ） ℃, （20 - 25 ） ℃ and （30 - 35 ） ℃ and transported to the laboratory for culture. They were divided into three groups randomly in an optimized transport temperature, kept in physiological saline at4 ℃, （14 -18） ℃ and （25 -30） ℃ respectively; 15 - 17 h later, the ovaries were cutured for the maturation and parthenogenetic activation of oocytes. The results showed that the optimal transport temperature of ovaries was 20 -25 ℃, when the maturation rate and blastocysts rate reached 67.44% and 35.93% respectively. Incubating sheep ovaries at （ 14 - 18 ） ℃ for （15 -17） h did not reduce the maturation rate （61.81%） and blastocysts rate （29.03%） of oocyte significantly. However, incubating sheep ovaries at 4 ℃ or （25 -30） ℃ significantly reduced the maturation rate （41.90%, 18.40% ） and cleavage rate （9.09%, 13.04% ）, and the ovaries could not develop into blastocysts at these two temperature ranges. It can be concluded that the optimal transport temperature of the sheep ovaries is 20 - 25 ℃, and incubation at 14 - 18 ℃ overnight in vivo does not affect the developmental competence of oocytes.

关键词： Sheep ovary Storage temperature Transport temperature Oocytc developmental competence

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Association between mitochondrial DNA haplotype compatibility and increased efficiency of bovine intersubspecies cloning

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Journal of Genetics and Genomics 2011年第1期38卷 21-28页

作者： Hao Yan Zhonghai Yan Qingwen Ma Fei Jiao Shuzhen Huang Fanyi Zeng Yitao Zeng Shanghai Institute of Medical Genetics Shanghai Jiao Tong University 24/1400 West Beijing Road Shanghai 200040 China The Key Lab of Embryo Molecular Biology Ministry of Health China and Shanghai Lab of Embryo and Reproduction Engineering 24/1400 West Beijing Road Shanghai 200040 China Institute of Medical Science Shanghai Jiao Tong University School of Medicine 280 South Chongqing Road Shanghai 200025 China

Reconstructed embryos derived from intersubspecies somatic cell nuclear transfer （SCNT） have poorer developmental potential than those from intrasubspecies SCNT. Based on our previous study that Holstein dairy bovine （HD） mitochondrial DNA （mtDNA） haplotype compatibility between donor karyoplast and recipient cytoplast is crucial for SCNT embryo development, we performed intersubspecies SCNT using HD as donor karyoplast and Luxi yellow heifer （LY） as recipient cytoplast according to mtDNA haplotypes determined by polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP） analysis. The results demonstrated that intersubspecies mtDNA homotype SCNT embryos had higher pre- and post-implantation developmental competence than intrasubspecies mtDNA heterotype embryos as well as improved blastocyst reprogramming status, including normal H3K9 dimethylation pattern and promoter hypomethylation of pluripotent genes such as Oct4 and Sox2, suggesting that intersubspecies SCNT using LY oocytes maintains HD cloning efficiency and may reprogram HD nuclei to develop into a normal cloned animal ultimately. Our results indicated that karyoplast-cytoplast interactions and mtDNA haplotype compatibility may affect bovine intersubspecies SCNT efficiency. This study on bovine intersubspecies SCNT is valuable for understanding the mechanisms of mtDNA haplotype compatibility between karyoplast and cytoplast impacting the bovine SCNT efficiency, and provides an alternative and economic resource for HD cloning.

关键词： Somatic cell nuclear transfer (SCNT) Mitochondria mtDNA haplotype Epigenetic modification Bovine Intersubspecis Intrasubspecis developmental competence

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Expression of Target Genes in Cumulus Cells Derived from Human Oocytes with and without Blastocyst Formation

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Reproductive and developmental Medicine 2019年第2期3卷 84-88页

作者： Tuan-Ping Zhou Di Zhang Ling-Bo Cai Yi-Xin Xu Li Shao Kai-Lu Liu Jia-Yin Liu Yu-Gui Cui Ling Wang Ri-Cheng Chian State Key Laboratory of Reproductive Medicine Clinical Center for Reproductive MedicineThe First Affiliated Hospital of Nanjing Medical UniversityNanjing 211166China Center for Reproductive Medicine Shanghai 10th People’s Hospital of Tongji UniversityShanghai 200072China

Objective:The transcriptional profile of cumulus cells(CCs)during oocyte maturation provides information for predicting oocyte developmental *** previous study using a mouse model indicated that there were nine different genes related to oocyte development potential expressed in CCs during oocyte *** purpose of this study was to elucidate whether gene expression levels of CCs during oocyte maturation are associated with oocyte developmental ***:The human CCs collected from each oocyte were divided into two groups after tracking depending on whether or not they developed to the blastocyst stage:(1)the oocytes were developed to blastocyst stage after fertilization(B+)and(2)the oocytes were not developed to blastocyst stage after fertilization(B−).The expression levels of the nine selected genes(ARRB1,ATP2C1,CDH5,CNTNAP1,LGR4,MKLN1,RHOBTB1,SIX2,and SMC2)were *** were obtained from 29 women who were undergoing intracytoplasmic sperm injection treatment *** reverse transcriptase-polymerase chain reaction analysis was performed on cumulus masses collected before *** sample was run three *** significant differences in mRNA expression of the target genes in independent samples were evaluated by two-tailed Student’s t-test,and Pdevelopmental competence before insemination and fertilization.

关键词： Cumulus Cells developmental competence In vitro Maturation Oocyte Quality Oocyte

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