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Analysis of nickel distribution by synchrotron radiation X-ray fluorescence in nickel-induced early- and late-phase allergic contact dermatitis in Hartley guinea pigs

引用

Chinese Medical Journal 2019年第16期132卷 1959-1964页

作者： Shan-Qun Jiang Xiang-Yu Wu Jin-Lyu Sun Guang Chen Rui Tang Zhi Li Ruo-Yao Wei Lan Liang Xian-Jie Zhou Dong-Liang Chen Jun Li Hong Gao Jing Zhang Zuo-Tao Zhao School of Life Science Anhui University111 Jiulong RoadHefeiAnhui 230601China Department of Allergy Peking Union Medical College HospitalChinese Academy of Medical Sciences (CAMS)& Peking Union Medical College (PUMC)Beijing Key Laboratory of Precision Medicine for Diagnosis and Treatment on Allergic DiseasesBeijing 100730China Beijing Synchrotron Radiation Facility (BSRF) Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China The Institute of Laboratory Animal Science Chinese Academy of Medical Sciences (CAMS)& Peking Union Medical College (PUMC)Beijing 100021China Laboratory Animal Center Peking UniversityBeijing 100083China Department of Dermatology Peking University First HospitalPeking UniversityBeijing Key Laboratory of Molecular Diagnosis on DermatosesBeijing 100034China

Background: Nickel-induced allergic contact dermatitis (Ni-ACD) is a global health problem. More detailed knowledge on the skin uptake of haptens is required. This study aimed to investigate the penetration process and distribution of nickel in skin tissues with late phase and early phase of Ni-ACD to understand the mechanisms of metal allergy. Methods: Forty Hartley guinea pigs were divided into four groups according to the NiSO4 sensitizing concentration and the NiSO4 challenged concentration: the 5% NiSO4-group, 5% to 10%(sensitization-challenge;late phase group);10% NiSO4-group, 10% to 10%(sensitization-challenge;early-phase group);and the positive and negative controls. Pathological biopsies were performed on each group. The depth profile of nickel element concentration in the skin of guinea pigs was detected by synchrotron radiation micro X-ray fluorescence spectroscopy (SR-μ-XRF) and micro X-ray absorption near-edge spectroscopy (μ-XANES). Results: In each section, the nickel element concentration in both the 5% NiSO4-group and 10% NiSO4-group was significantly higher than that in the negative control group. In the upper 300-μm section of skin for the early phase group, the nickel element concentration was significantly higher than that in the lower section of skin. In deeper sections (>200 μm) of skin, the concentration of nickel in the early phase group was approximately equal to that in the late phase group. The curve of the late phase group was flat, which means that the nickel element concentration was distributed uniformly by SR-μ-XRF. According to the XANES data for the 10% NiSO4 metal salt solution, structural changes occurred in the skin model sample, indicating that nickel was not present in the Ni^2+ aqueous ionic state but in the nickel-binding protein. Conclusions: This study showed that the distribution of the nickel element concentration in ACD skin tissue was different between the early phase and late phase groups. The nickel element was not p

关键词： Synchrotron radiation micro X-ray fluorescence spectroscopy Micro X-ray absorption near-edge spectroscopy dermatitis, Allergic contact Nickel-induced allergic contact dermatitis Dermatology

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