检索结果-南通市图书馆

<i>Arthrobacter arilaitensis</i>Re117 as a Source of Solvent-Stable Proteases: Production, Characteristics, Potential Application in the deproteinization of Shrimp Wastes and Evaluation in Liquid Laundry Commercial Detergents

引用

Advances in Bioscience and Biotechnology 2015年第2期6卷 105-119页

作者： Rayda Siala Ines Hammemi Sabrine Sellimi Tatiana Vallaeys Alya Sellami Kamoun Moncef Nasri Laboratory of Enzyme Engineering and Microbiology National School of Engineering of Sfax (ENIS) University of Sfax Sfax Tunisia Unité de Recherche Protection des Plantes Cultivées et Environnement Institut de l’Olivier Sfax Tunisia Laboratoire Ecosystèmes Lagunaires UMR 5119 Université de Montpellier Montpellier France

The present study describes the characterization of crude protease extract from Arthrobacter arilaitensis Re117 and its evaluation in solid and liquid detergent. One caseinolytic protease clear band was observed in zymogram. The crude alkaline protease showed optimum activity at pH 9.0 and 50°C, and it was highly stable over a wide range of pH from 8.0 to 9.0. Proteolytic enzymes showed extreme stability towards non-ionic surfactants (Tween 80, Tween 20 and Triton X-100) and stimulate activity towards oxidizing agents such as sodium perborate. They also showed high stability and compatibility with various laundry solid detergents from Tunisian market. The protease of A. arilaitensis Re117, was also tested for shrimp waste deproteinization to produce chitin. The protein removal with a ratio E/S of 20 was about 83%. The novelties of the Re117 protease include its high stability to organic solvents and surfactants. These unique properties make it an ideal choice for application in detergent formulations and enzymatic peptide synthesis. In addition, the enzyme may find potential applications in the deproteinization of shrimp wastes to produce chitin.

关键词： A. arilaitensis Shrimp Wastes Enzymatic deproteinization Organic Solvent-Stable Protease Laundry Detergents Compatibility

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Immunological study on the transplantation of an improved deproteinized heterogeneous bone scaffold material in tissue engineering

引用

Chinese Journal of Traumatology 2008年第3期11卷 141-147页

作者：刘雷裴福兴屠重棋周宗科李起鸿 Department of Orthopedics West China HospitalSichuan University Chengdu 610061 China Center for Joint Surgery Southwest Hospital ThirdMilitary Medical University Chongqinq 400038 China

Objective： To observe the immune response after the transplantation of a deproteinized heterogeneous bone scaffold and provides the theoretic reference for clinical practice. Methods： The fresh pig bone and deproteinized bone were transplanted respectively to establish BABL/C thigh muscle pouches model of male mice and take the samples for detection at 1, 2, 4, 6 weeks after operation. Lymphocyte stimulation index, subset analysis, serum specific antibody IgG, cytokine detection and topographic histologic reaction after implantation were investigated. Results： After the transplantation of deproteinized bone, lymphocyte stimulation index, CD4^＋ and CD8^＋ T-lymphocyte subsets, serum specific antibody IgG and cytokines in deproteinized bone group were significantly lower than those in fresh pig bone group at each time point （P〈0.05）. The histological examination found that in fresh bone group at each time point, a large quantity of inflammatory cells infiltrated in the surrounding of bone graft, and they were mainly lymphocytes, including macrophages and monocytes. In deproteinized bone group, there were few inflammatory cells infiltration around bone graft one week after operation. The lymphocytes were decreased as time went by. At 6 weeks, fibroblasts and fibrous tissue grew into the graft, and osteoclasts and osteoprogenitor cells appeared on the verge. Conclusions： The established heterogeneous deproteinized bone has low immunogenicity and is a potentially ideal scaffold material for bone tissue engineering.

关键词： Heterogeneous bone deproteinization Tissue engineering Transplantation Immune reaction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：