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Hematomas and Limb Skeletal Malformations in Chicken Embryos Following Exposure to 5-Fluoro-2'-deoxyuridine

Biomedical and Environmental Sciences 1998年第1期11卷 15-30页

作者： ROBERT S. NOLAN GARRY SCHWARTZ SEAN FARQUHARSON DAVID R.HOOTNICK E. MARK LEVINSOHN MARY MILLER LISA A. MANZ AND DAVID S. PACKARD, JR (Departments of Anatomy and Cell Biology, Orthopedic Surgery,and Pediatrics, State University of New York Health Department of Anatomy and Cell Biology State University of New York Health Science Center Syracuse 13210 USA.

Vascular injury or interruption may play a role in vertebrate limb teratogenesis. Since 5fluoro- 2'- deoxyuridine (FdU) can cause vascular injury in the murine limb and skull prior to the appearance of skeletal malformations in these structures, we studied the effects of this chemical on skeletal development in the chick embryo and noted any vascular injury. The yolk sacs of day three ehick embryos (Hamburger and Hamilton states 17-19) were injected with solutions of vary concentrations of FdU in saline. The embryos developed until the 10th day of incubation when they were fixed for study. Uninjected, saline injected, and sham injected control embryo were similarly fixed. Upon gross inspection, frequent diffuse and saccular hernatomas, as well as fluid-filled blisters, were noted in the limbs of embryos treated with FdU. After the embryos were fixed and cleared, and the skeletons stained, significant skeletal malformations were observed in these limbs. Bony elements of both the upper and lower limbs were affected in at least some of the embryos. The combination of FdU-induced hematomas and blisters with associated skeletal malformations in the same regions of some embryos suggests a relationship between these phenomena.

关键词： Hematomas and Limb Skeletal Malformations in Chicken Embryos Following Exposure to 5-Fluoro-2 deoxyuridine

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Embryonic Hypertension Following Exposure to Teratogenic Doses of 5-Fluoro-2'-deoxyuridine

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Biomedical and Environmental Sciences 1996年第4期9卷 408-417页

作者： VINU D. PATEL ROBERT S. NOLAN NORMAN HU EDWARD B. CLARKA DAVID R. HOOTNICK E. MARK LEVINSOHN AND DAVID S. PACKARD, JR.(Departments of Obstetrics and Gynecology, Anatomy and Cell Biotogy, and Pediatrics, Stale University of New York Health Science C Department of Obstetrics and Gynecology State University of New York Health Science Center Syracuse New York 13210 USA.

The teratogenicity of 5-fluoro-2'-deoxyuridine (FdU) is well established. Previously, we have demonstrated that teratogenic doses of FdU produce hematomas and suggested that those hematomas produced skeletal malformations in chicken embryos. In this study, the cardiovascular effects of teratogenic doses of FdU in chicken embryos were studied. A dose of either 0.026 μg FdU or 0.030 μg FdU was injected into the yolk sacs of fertile chicken eggs containing embryos at Hamburger and Hamilton stages 17-19 of development. The embryos were then returned to the incubator. Aortic systolic and diastolic blood pressure,blood velocity and heart rate were measured at stages 21, 24 or 27 using a servonull system and Doppler ultrasound. In addition, mean arterial blood pressure, blood flow, and stroke volume were calculated from these data. Similar data were also recorded from uninjected and saline injected control embryos. Systolic and mean arterial blood pressures were significantly increased in FdU-treated embryos at stage 27. The other parameters measured or calculated were not significantly different from control embryos. Our study suggests that elevated systolic blood pressure in chicken embryos treated with FdU may lead to hematoma formation and subsequent birth defects

关键词： deoxyuridine Embryonic Hypertension Following Exposure to Teratogenic Doses of 5-Fluoro-2

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The observation of transplanted embryonic motoneurons in the denervated muscles of adult rats

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Chinese Medical Journal 1998年第1期111卷 63-66页

作者：姜广良顾玉东 China 200040

OBJECTIVE: To observe the survival of embryonic motoneurons after they were transplanted into the denervated skeletal muscles and to find a new method to retard the atrophy of denervated muscles. METHODS: Dissociated embryonic motoneurons prelabled with 5-bromo-2'-deoxyuridine (Brdur) on the embryonic days 12 were injected into the denervated gastrocnemius muscles of adult rats. Then gastrocnemius muscles were processed with Nissl staining, acetylcholinesterase staining and Brdur immunocytochemical staining to show the implanted motoneurons at 9 and 22 weeks post-transplantation. Myofibrillar ATPase staining was used to show the morphology of muscle fibers. The rats in experimental group were implanted with embryonic motoneurons in the predenervation muscles, while the rats in control group were injected with just culture medium without motoneurons. RESULTS: Embryonic motoneurons survived, developed and extended long axons to form neuromuscular junctions with the denervated muscles. The differentiation of muscle fibers and fiber type grouping occurred among bigger fibers in experimental group. The transverse area was smaller and there was no apparent fiber type grouping in control group. CONCLUSIONS: Embryonic motoneurons can survive, develop and reinnervate denervated muscles after being transplanted into denervated muscles. It is worth further investigating on ameliorating the atrophy of denervated muscle.

关键词： Animals deoxyuridine Embryo Female Motor Neurons Muscle Denervation Muscle, Skeletal Pregnancy Rats Rats, Sprague-Dawley Research Support, Non-U.S. Gov't

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