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Journal of Forensic Science and Medicine 2023年第1期9卷 64-69页

作者： Lei Miao Jia-Hui Yuan Ke-Lai Kang Jie Zhao Chi Zhang Le Wang Key Laboratory of Forensic Genetics of Ministry of Public Security Institute of Forensic ScienceMinistry of Public SecurityBeijingChina Department of Forensic Medicine School of Forensic MedicineKunming Medical UniversityKunmingYunnanChina

deoxyribonucleic acid(DNA)genetic markers and ribonucleic acid(RNA)molecular markers have been widely used in forensic practices including individual identification,parentage testing,body fluid identification,determination of the age of stains,and molecular pathological *** information of biological evidence and their interrelation could be revealed by the integrated detection of DNA/RNA *** integrated detection workflow aims to simplify working procedures,reduce time consuming and save valuable samples collected from crime ***-generation sequencing(NGS)may be an effective method for integrated DNA/RNA *** this review,DNA/RNA co-extraction strategies,simultaneous detection methods based on capillary electrophoresis were *** on NGS-based integrated detection methods of DNA and RNA markers was reviewed to provide a reference for forensic medicine researches and applications.

关键词： Forensic genetics deoxyribonucleic acid ribonucleic acid integrated detection methods next-generation sequencing

Sex Determination using Primary and Permanent Teeth under Different Environment Conditions:A deoxyribonucleic acid-based Study

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Journal of Forensic Science and Medicine 2023年第3期9卷 250-256页

作者： Jaya Dubey Jigna S.Shah Department of Oral Medicine and Radiology Government Dental College and HospitalAhmedabadGujaratIndia

Aims: The purpose of this study was to determine the sex of primary and permanent tooth pulp tissue under different environmental conditions using deoxyribonucleic acid (DNA) analysis and the polymerase chain reaction (PCR) amplification method. Materials and Methods: For this study, 40 permanent and primary teeth (20 males and 20 females) that were scheduled for extraction were used. For 6 months, the teeth were divided into four groups of ten (5 males and 5 females) based on different environmental conditions. The pulp was extracted from each tooth, DNA was isolated using QIAGEN’s DNeasy Blood and Tissue Kit, and sex was determined using a PCR technique. Results: DNA was extracted from all 40 samples subjected to various environmental conditions, but sex was correctly determined in only 90% of the samples. Conclusions: PCR-based sex determination using primary and permanent teeth under different environmental conditions for up to 6 months is a reliable technique with high accuracy.

关键词： Amelogenin gene deoxyribonucleic acid forensic gender determination polymerase chain reaction

Spermine induced reversible collapse of deoxyribonucleic acid-bridged nanoparticle-based assemblies

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Nano Research 2018年第1期11卷 383-396页

作者： Kristian L. Goeken Richard B. M. Schasfoort Vinod Subramaniam Ron Gill MIRA Institute for Biomedical Technology and Technical Medicine University of Twente Dfienerlolaan 5 7522 NB Enschede the Netherlands Vrije UniversiteitAmsterdam De Boelelaan 1105 1081 HVAmsterdam the Netherlands Saxion University of Applied Sciences M. H. Tromplaan 28 7513 A8 Enschede the Netherlands

DNA-linked 2D and 3D nano-assemblies find use in a diverse set of applications, ranging from DNA-origami in drug delivery and medical imaging, to DNA-linked nanoparticle structures for use in plasmonics and （bio）sensing. However, once these structures have been fully assembled, few options are available to modulate structure geometry. Here, we investigated the use of the polycation spermine to induce DNA collapse in small oligonucleotide-linked （54 bp） gold nanoparticle structures by monitoring shifts in the localized surface plasmon resonance （LSPR） peak and by comparing the data with finite-difference time-domain （FDTD） simulations. Our data shows that low concentrations of spermine can be applied to induce large changes in DNA conformation, leading to a significant reduction in interparticle distance （from - 25 to - 3 nm） and enhanced plasmonic coupling. The DNA collapse is near-instantaneous and reversible, and its application at low and high DNA densities is demonstrated with surface plasmon resonance imaging （SPRi）, showing the potential of spermine to dynamically modulate distances and geometry in DNA-based nano-assemblies.

关键词： nanoparticles spermine deoxyribonucleic acid localized surface plasmonresonance surface plasmon resonanceimaging

A general synthetic strategy for N, P co-doped graphene supported metal-rich noble metal phosphides for hydrogen generation

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Green Energy & Environment 2024年第1期9卷 152-162页

作者： Jingwen Ma Xiang Li Guangyu Lei Jun Wang Juan Wang Jian Liu Ming Ke Yang Li Chunwen Sun School of Chemical and Environmental Engineering China University of Mining and Technology(Beijing)Beijing100083China State Key Laboratory of Heavy Oil Processing China University of PetroleumBeijing102249China Lab of Advanced Nano-structures&Transfer Processes Department of Chemical EngineeringTianjin UniversityTianjin300354China PetroChina Planning and Engineering Institute Beijing100083China

The exploitation of electrocatalysts with high activity and durability for HER is desirable for future energy systems,but it is still a *** have attracted increasing attentions,but the preparation process often needs toxic regents or dangerous reaction ***,we develop a general green method to fabricate metal-rich NMPs anchored on NPG through pyrolyzing DNA cross-linked *** obtained Ru_(2) P-NPG exhibits an ultrasmall overpotential of 7 mV at 10 mA cm^(2) and ultralow Tafel slope of 33 mV dec^(-1) in 1.0 mol L?1 KOH,even better than that of commercial Pt/*** addition,Ru 2 P-NPG also shows low overpotentials of 29 and 78 mV in 0.5 mol L^(-1) H_(2)SO_(4) and 1.0 mol L^(-1) PBS,*** superior activity can be attributed to the ultrafine dispersion of Ru 2 P nanoparticles for more accessible sites,more defects formed for abundant active sites,the two-dimensional plane structure for accelerated electron transfer and mass transport,as well as the regulation of electron distribution of the ***,the synthetic method can also be applied to prepare other metal-rich noble metal phosphides(Pd_(3)P-NPG and Rh_(2)P-NPG),which also exhibits high activity for *** work provides an effective strategy for designing NMP-based electrocatalysts.

关键词： Noble metal phosphides Electrocatalyst deoxyribonucleic acid Hydrogen evolution pH universal

Sequential extraction of RNA,DNA and protein from cultured cells of the same group

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World Journal of Methodology 2023年第5期13卷 484-491页

作者： Ying-Yu Cui Department of Cell Biology Institute of Medical GeneticsKey Laboratory of Arrhythmias of the Ministry of Education of ChinaTongji University School of MedicineShanghai 200331China

BACKGROUND Efficient extraction of nucleic acids and proteins(ENAP)from cells is a prerequisite for precise annotation of gene function,and has become laboratory routine for revealing the mysteries of ***,cell samples are often from different culture dishes,resulting in inevitable experimental errors and sometimes poor *** To explore a method to improve the efficiency of ENAP,minimizing errors in ENAP processes,enhancing the reliability and repeatability of subsequent experimental *** A protocol for the sequential isolation of RNA,DNA,and proteins from the same cultured HepG2 cells using RNAzol reagent is presented *** first step involves culturing HepG2 cells to the exponential phase,followed by the sequential isolation of RNA,DNA,and proteins from the same cultured cells in the second *** yield of nucleic acids and proteins is detected in the third step,and their purity and integrity are verified in the last *** The procedure takes as few as 3-4 d from the start to quality verification and is highly *** contrast to the existing kits and reagents,which are primarily based on independent isolation,this RNAzol reagent-based method is characterized by the sequential isolation of RNA,DNA,and proteins from the same cells,and therefore saves time,and has low cost and high *** The RNA,DNA,and proteins isolated using this method can be used for reverse transcription-polymerase chain reaction,polymerase chain reaction,and western blotting,respectively.

关键词： Sequential extraction Ribonucleic acid deoxyribonucleic acid Protein Cultured cells

Extraction and DNA Digestion of 5'-Phosphodiesterase from Malt Root

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Tsinghua Science and Technology 2008年第4期13卷 480-484页

作者：邹和昌蔡光圻蔡文李海龙顾益朴龙斗孟凡国 Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Yangtze Delta Region Institute of Tsinghua University Jiaxing 314050 China Zhejiang Jiaxing Pharmacology Corporation Jiaxing 314001 China Yangtze Delta Region Institute of Tsinghua University Jiaxing 314050 China

This study investigated the extraction of 5＇-phosphodiesterase from malt root and the degradation of nucleic acids by this enzyme. The extraction used grade precipitation with ammonium sulfate and enzymatic hydrolysis. Samples were assayed using the modified Bradford method and high performance liquid chromatography. The results show that 5＇-phosphodiesterase is isolated by grade precipitation with 30% and 80% saturation of ammonium sulfate and can be utilized to degrade deoxyribonucleic acid. The hydrolysate has four kinds of deoxynucleotides： 5＇-dCMP, 5＇-dTMP, 5＇-dAMP, and 5＇-dGMP. The optimum reaction temperature is 70℃, and the optimum pH is 5.5-6.0 for the reaction. The percentage of deoxynucleotides indicated by the China Pharmacopoeia （2000 edition） in the product is over 70%. The extraction of 5＇-phosphodiesterase from malt root is shown to be possible and economical. Products from the enzYmatic hydrolysate of DNA meet the pharmacopoeia.

关键词： 5'-phosphodiesterase malt root deoxyribonucleic acid

CORRELATIONSHIP　BETWEEN　CELLULAR　DNA　AND　AgNOR　PROTEIN　CONTENT　IN　THE　DEVELOPING　COURSE　OF　COLORECTAL　ADENOCARCINOMA

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Chinese Journal of Cancer Research 1995年第4期7卷 280-283页

作者：谢尧屈汉廷 Department of Clinical Medicien People's Hospital Beijing Medical University Beijing

Cellular NDA and AgNOR Protein contents were evaluated byautomatic image analysis in tissue sections stained hy combined Feulgen-AgNOR staining method in 9 normal colonic mucosae, 45 colorectal adenomas and 27 adenocarcinomas. The results indicated that during the course that the normal colonical mucosa developed to colorectal adenocarcinoma via adenoma the DNA and AgNOR protein contents increased gradually and there were very significant correlationships between the DNA and the AgNOR protein contents of adenoma group and adenocarcinoma group. However, there were considerahle overlaping in the DNA or AgNOR Protein content and considerahle overlaping cases between adenoma and normal colonic mucosa groups and between adenoma and adenocarcinoma groups. But the overlaping scope in NDA and AgNOR Protein content and the number of overlaping cases were reduced significantly by assessing the correlationship between the DNA and AgNOR protein content. Therefore, it is much more reliable to distinguish colorectal adenomas from adnocarcinomas by using the correlationship between the cellular DNA and the AgNOR Protein contents in the same specimens.

关键词： Colorectal Tumor Adenoma,Adenocarcinoma deoxyribonucleic acid Tumor,AgNOR protein Nucleolar Organizer Regions

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DNA对聚苯胺电化学行为的影响

分析化学 2013年第12期41卷 1953-1954页

作者：魏仲航陈丽波姜涛吉林大学中日联谊医院

1引言导电聚合物具有特殊的分子结构及良好的导电性能。在聚苯胺、聚噻吩和聚吡咯三类导电聚合物中,聚苯胺由于其良好的环境稳定性、可控的导电性以及多样的氧化还原性而备受关注[1],被广泛用于纳米复合物的制备及应用等[2]。通常,聚苯... 详细信息

1引言导电聚合物具有特殊的分子结构及良好的导电性能。在聚苯胺、聚噻吩和聚吡咯三类导电聚合物中,聚苯胺由于其良好的环境稳定性、可控的导电性以及多样的氧化还原性而备受关注[1],被广泛用于纳米复合物的制备及应用等[2]。通常,聚苯胺需要pH<4的条件以保持其电化学活性,在一定程度上限制了其应用。一些功能化基团,如COOH,SO3H及BO2H2等,被引入到聚苯胺链中,"掺杂"的聚苯胺在较宽的pH范围内改善了其溶解性、氧化还原稳定性及电化学活性

关键词： Polyaniline deoxyribonucleic acid Cyclic voltammogram

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Computational Molecular Bioscience 2020年第3期10卷 61-72页

作者： Dipendra C. Sengupta Matthew D. Hill Kevin R. Benton Hirendra N. Banerjee Department of Mathematics Computer Science & Engineering Technology Elizabeth City State University Elizabeth City North Carolina USA Department Natural Sciences Elizabeth City State University Elizabeth City North Carolina USA

The novel coronavirus (SARS-COV-2) is generally referred to as Covid-19 virus has spread to 213 countries with nearly 7 million confirmed cases and nearly 400,000 deaths. Such major outbreaks demand classification and origin of the virus genomic sequence, for planning, containment, and treatment. Motivated by the above need, we report two alignment-free methods combing with CGR to perform clustering analysis and create a phylogenetic tree based on it. To each DNA sequence we associate a matrix then define distance between two DNA sequences to be the distance between their associated matrix. These methods are being used for phylogenetic analysis of coronavirus sequences. Our approach provides a powerful tool for analyzing and annotating genomes and their phylogenetic relationships. We also compare our tool to ClustalX algorithm which is one of the most popular alignment methods. Our alignment-free methods are shown to be capable of finding closest genetic relatives of coronaviruses.

关键词： Covid-19 Chaos Game Representation deoxyribonucleic acid Phylogenetic Analysis Shannon Entropy

Clinical Study on the Piweiping Capsule (脾胃平胶囊) in TreatingPatients with Metaplasia of Gastric Mucosa

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Chinese Journal of Integrative Medicine 1998年第2期4卷 84-88页

作者：尹光耀何雪芬村永清尹玉芬蓝士宝李葆富杨仲臣

Objective: To evaluate the therapeutic effect of Piweiping capsule (PWPC) in treating patients with metaplasia of gastric mucosa and explore its mechanism. Method: One hundred and forty-three patients were diagnosed as chronic superficial atrophic gastritis of gastric antrum or with metaplasia by means of gastroscopic observation with pathological biopsy examination. They were divided into 4 types (typeⅠ -Ⅳ ) according to Syndrome Differentiation of traditional Chinese medicine, and each type was subdivided according to treatment into 2 groups, 75 in the PWPC group and 68 in the San jiu Weitai (SJWT, 三九胃泰) group. Patients of PWPC group were in accordance with the type to which they belong treated with PWPC Ⅰ, Ⅱ, Ⅲ, Ⅳ respectively, while patients of SJWT group were all treated with SJWT. After 3 - 6 months of treatment, the clinical effect, pathological examination of gastric mucosa and some other parameters were observed. Results:The improvements of gastric mucosa cyclic adenosing monophosphate (cAMP ), deoxyribonucleic acid (DNA), 3H-thymopyrimidine nucleoside and lymphocyte transformation of the treatment group were all superior to those of SJWT group, P＜ 0. 05 - 0. 001. In the PWPC group, the curative rate and total effective rate on symptom were 49. 3 % and 92. 0% respectively, and the curative rate and total effective rate on intestinal metaplasia were 45. 3 % and 62. 7%. As for the SJWT group, they were 29. 4%, 67. 6%, 10. 3% and 29. 4% respectively. The difference between the two groups was significant, P ＜ 0. 001. Conclusion: PWPC could reverse the intestinal metaplasia through improving the gastric cAMP, DNA and strengthening the cellular immunity and the effect would be enhanced when the treatment was based on Syndrome Differentiation.

关键词： intestinal metaplasia of gastric mucosa deoxyribonucleic acid cyclic adenosine monophosphate Piweiping capsule