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Effect of Different Irradiation Times on the Occlusion of dentinal Tubules When Using a Nd:YAG Laser: An <i>in Vitro</i>SEM Study

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Open Journal of Stomatology 2015年第3期5卷 72-79页

作者： Xiaoyu Guo Jinhua Yu Roger J. Smales Huifen Chen Haiyan Si Younong Wu Department of Endodontics Affiliated Hospital of Stomatology Nanjing Medical University Nanjing China School of Dentistry Faculty of Health Sciences The University of Adelaide Adelaide Australia Institute of Stomatology Nanjing Medical University Nanjing China

Objective: To evaluate the effect of different irradiation times on the occlusion of dentinal tubules when using Nd:YAG laser. Background data: Dentin hypersensitivity is a frequent problem that has limited treatment success despite many chemical and physical therapies. Methods: Four coronal dentin disks 2 mm thick were cut with a low-speed diamond saw from four freshly extracted intact first molars. The coronal dentin surface of each disk was divided into four regions, each approximately 2 mm × 3 mm. The dentin surfaces were treated with 27% EDTA then the four regions irradiated separately in a randomized pattern with a Nd:YAG laser (120 mJ, 5 Hz), using irradiation times of 0 s, 20 s, 40 s and 60 s, representing laser energies of 0 J/cm2, 200 J/cm2, 400 J/cm2 and 600 J/cm2, respectively. SEM photomicro-graphs were taken at 1500× and 3000× to calculate the dentinal tubule orifice occlusion rates and to measure the tubule diameters, respectively. Results: For the laser irradiation times of 0 s, 20 s, 40 s and 60 s, the corresponding dentinal tubule occlusion rates were 2.05 (SD 0.29)%, 10.01 (1.71)%, 23.58 (2.51)% and 90.23 (2.24)%, respectively;and the tubule diameters were 4.18 (0.32) μm, 3.46 (0.24) μm, 1.69 (0.32) μm and 0.12 (0.02) μm, respectively. There were significant differences among all groups for both measured variables (p ≤ 0.005). Conclusions: Within the limitations of this in Vitro study, when using a Nd:YAG laser at 120 mJ and 5 Hz, an irradiation time of 60 s achieved the best sealing of the coronal dentinal tubule orifices.

关键词： Nd:YAG Laser dentinal Tubule Scanning Electron Microscope

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