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Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid dendrobium nobile

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Plant Diversity 2023年第3期45卷 326-336页

作者： Yu-Wen Zhang Yu-Cen Shi Shi-Bao Zhang Key Laboratory of Economic Plants and Biotechnology Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina Yunnan Key Laboratory for Wild Plant Resources Kunming 650201YunnanChina University of Chinese Academy of Sciences Beijing 100049China Lijiang Forest Biodiversity National Observation and Research Station Kunming Institute of BotanyChinese Academy of SciencesLijiang 674100YunnanChina

dendrobium nobile is an important medicinal and nutraceutical herb.Although the ingredients of D.nobile have been identified as polysaccharides,alkaloids,amino acids,flavonoids and bibenzyls,our understanding of the metabolic pathways that regulate the synthesis of these compounds is limited.Here,we used transcriptomic and metabolic analyses to elucidate the genes and metabolites involved in the biosynthesis of carbohydrate and several secondary metabolites in the stems of D.nobile.A total of 1005 metabolites and 31,745 genes were detected in the stems of D.nobile.The majority of these metabolites and genes were involved in the metabolism of carbohydrates(fructose,mannose,glucose,xylulose and starch),while some were involved in the metabolism of secondary metabolites(alkaloids,β-tyrosine,ferulic acid,4-hydroxybenzoate and chrysin).Our predicted regulatory network indicated that five genes(AROG,PYK,DXS,ACEE and HMGCR) might play vital roles in the transition from carbohydrate to alkaloid synthesis.Correlation analysis identified that six genes(ALDO,PMM,BGLX,EGLC,XYLB and GLGA) were involved in carbohydrate metabolism,and two genes(ADT and CYP73A) were involved in secondary metabolite biosynthesis.Our analyses also indicated that phosphoenol-pyruvate(PEP) was a crucial bridge that connected carbohydrate to alkaloid biosynthesis.The regulatory network between carbohydrate and secondary metabolite biosynthesis established will provide important insights into the regulation of metabolites and biological systems in dendrobium species.

关键词： dendrobium nobile Transcriptome Metabolome Polysaccharides Alkaloids Secondary metabolite biosynthesis

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Influence of light intensity and water content of medium on total dendrobine of dendrobium nobile Lindl

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Asian Pacific Journal of Tropical Medicine 2017年第11期10卷 1095-1100页

作者： Jin-Ling Li Zhi Zhao Hong-Chang Liu Chun-Li Luo Hua-Lei Wang Guizhou University GuiyangGuizhou 550025China Guizhou Key(Engineering)Laboratory for Propagation and Cultivation of Medicinal Plants Guiyang 550025China

Objective: To ascertain the influence of light intensity and water content of medium on the total dendrobine of dendrobium nobile(D. nobile).Method: The principal component analysis combined with total dendrobine accumulation was conducted to assess the yield and quality of D. nobile in all treatments. In the experiment, D. nobile plants were cultivated in greenhouse as tested materials, and complete test of 9 treatments was adopted with relative light intensities 75.02%, 39.74%,29.93% and relative water content of medium 50%, 65%, 80%. The plants were treated in June and harvested till December. Indexes including agronomic traits, fresh weight and dry weight of stem and leaf, ash content, extract, and dendrobine were measured.Results: Under the light intensity treatments of 75.02% with 50%, 65%, 80% water content of medium, the basal stems of plants were comparatively thicker with more leaves, and the fresh weight and dry weight of stems and leaves were significantly higher than other 6 treatments.Leaves in all treatments contained dendrobine. Under the light intensity treatments of 75.02%with 50%, 65%, 80% water content of medium, dendrobine content of leaves was lower while dendrobine contents of other treatments were more than 0.60%. After comprehensive assessment through the principal component analysis and total dendrobine accumulation, the results showed that 3 treatments with relative light intensity of 75.02% ranked the top three.Conclusions: In brief, the moderately strong light intensity and water content of medium from low to medium can facilitate the growth and yield of D. nobile plants, while light intensity from moderately weak to weak can enhance the dendrobine content.

关键词： dendrobium nobile 轻紧张浇媒介的内容全部的 dendrobine 主要部件分析

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Interaction between a Dark Septate Endophytic Isolate from dendrobium sp. and Roots of D. nobile Seedlings

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Journal of Integrative Plant Biology 2009年第4期51卷 374-381页

作者： Xiao-Qiang Hou Shun-Xing Guo Institute of Medicinal Plant Development the Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100094 China

Interactions between an isolate of dark septate endophytes （DSE） and roots of dendrobium nobile Lindl. seedlings are reported in this paper. The isolate was obtained from orchid mycorrhizas on dendrobium sp. in subtropical forest. The fungus formed typical orchid mycorrhiza in aseptic co-culture with D. nobile seedlings on modified Murashige-Skoog （MMS） medium. Anatomic observations of the infected roots showed that the DSE hyphae invaded the velamen layer, passed through passage cells in exodermis, entered the cortex cells, and then formed fungal pelotons of orchid mycorrhiza. D. nobile seedlings＇ plant height, stem diameter, new roots number and biomass were greatly enhanced by inoculating the fungus to seedlings. The fungus was identified as Leptodontidium by sequencing the polymerase chain reaction-amplified rDNA ITS1-5,8S-ITS2 （internal transcribed spacer （ITS）） regions and comparison with similar taxa.

关键词： dark septate endophytes dendrobium nobile fugal inoculation intemal transcribed spacer molecular identification archid mycorrhiza plant growth.

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A New Dendrobine-Type Alkaloid from dendrobium nobile

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Chinese Chemical Letters 2003年第3期14卷 278-279页

作者： Qun Fang LIU Wei Min ZHAO ShanghaiInstituteofMateriamedica ShanghaiInstituteforBiologicalSciencesChineseAcademyofSciencesShanghai200031 ShanghaiInstituteofMateriamedica ShanghaiInstituteforBiologicalScie

A new dendrobine-type alkaloid has been isolated from stems of dendrobium nobile lindl.. Its structure has been identified on the basis of spectroscopic method.

关键词： dendrobium nobile Orchidaceae alkaloid dendronobiline A.

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