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A 3D In-vitro model of the human dentine interface shows long-range osteoinduction from the dentine surface

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International Journal of Oral Science 2024年第2期16卷 225-237页

作者： William Macalester Asme Boussahel Rafael O.Moreno-Tortolero Mark R.Shannon Nicola West Darryl Hill Adam Perriman School of Cellular and Molecular Medicine Biomedical Sciences BuildingUniversity of BristolUniversity WalkBristolUnited Kingdom Bristol Centre for Functional Nanomaterials HH Wills Physics LaboratoryUniversity of BristolTyndall AvenueBristolUnited Kingdom Centre for Protolife Research School of ChemistryUniversity of BristolCantocks CloseBristolUnited Kingdom Max Planck-Bristol Centre for Minimal Biology School of ChemistryUniversity of BristolBristolUnited Kingdom Periodontology Bristol Dental SchoolUniversity of BristolLower Maudlin StreetBristolUnited Kingdom

Emerging regenerative cell therapies for alveolar bone loss have begun to explore the use of cell laden hydrogels for minimally invasive surgery to treat small and spatially complex maxilla-oral ***,the oral cavity presents a unique and challenging environment for in vivo bone tissue engineering,exhibiting both hard and soft periodontal tissue as well as acting as key biocenosis for many distinct microbial communities that interact with both the external environment and internal body systems,which will impact on cell fate and subsequent treatment ***,we design and bioprint a facile 3D in vitro model of a human dentine interface to probe the effect of the dentine surface on human mesenchymal stem cells(hMSCs)encapsulated in a microporous hydrogel *** demonstrate that the dentine substrate induces osteogenic differentiation of encapsulated hMSCs,and that both dentine andβ-tricalcium phosphate substrates stimulate extracellular matrix production and maturation at the gel-media interface,which is distal to the gel-substrate *** findings demonstrate the potential for long-range effects on stem cells by mineralized surfaces during bone tissue engineering and provide a framework for the rapid development of 3D dentine-bone interface models.

关键词： interface den surface

Rare dentin defects:Understanding the pathophysiological mechanisms of COLXVA1 mutations

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Genes & Diseases 2024年第5期11卷 1-5页

作者： Isaac Maximiliano Bugueno Tristan Rey Alexandra Jimenez-Armijo Marzena Kawczynski Naji Kharouf Marie-Cecile Maniere Yann Herault Agnes Bloch-Zupan Virginie HaushalterLaugel Institute of Genetics and Molecular and Cellular Biology(IGBMC) CNRS-UMR7104INSERM U1258Universitede StrasbourgIllkirch 67400France Faculty of dentistry Universitede StrasbourgStrasbourg 67000France Institute of Advanced Studies(USIAS) Universitede StrasbourgStrasbourg 67045France Reference Center for Rare Oral and dental Diseases(CRMR-O-Rares) Oral Medicine and Surgery DepartmentHopitaux Universitaires de Strasbourg(HUS)Strasbourg 67000France Rare Diseases Health Network“TETE COU”&European Reference Network ERN CRANIO Strasbourg 67000France “Institut de Genetique Medicale d’Alsace” Laboratory of Genetic DiagnosticHopitaux Universitaires de Strasbourg(HUS)Strasbourg 67000France Biomaterials and Bioengineering Laboratory Inserm UMR_S 1121Strasbourg 67000France Orofacial Development and Regeneration Faculty of MedicineInstitute of Oral BiologyCentre of Dental MedicineUniversity of ZurichZurich 8032Switzerland

dentin is a mineralized tissue with a chemical composition similar to bone but with a higher mineralized density and *** constitutes the central structure of the tooth between the internal pulp and external enamel toward the oral cavity or cementum toward the underlying *** dentin defects occur in a variety of rare genetic *** can manifest as“isolated”occurrences such as in dentinogenesis imperfecta(DI)or dentin dysplasia(DD)or can be associated with other symptoms in diseases such as osteogenesis imperfecta,Goldblatt syndrome,microcephalic osteodysplastic primordial dwarfism type Ⅱ,among others.

关键词： diseases den rigidity

二乙基亚硝胺(den)诱发λ/lacZ转基因小鼠微核和基因突变实验研究

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卫生研究 2005年第3期34卷 269-271页

作者：詹立张立实王莉朱玲张浩铃木孝昌本间正充吴德生四川大学华西医院国家(成都)中药安全性评价中心成都610041 四川大学华西公共卫生学院日本国立医药品食品卫生研究所遗传变异部

目的　应用λlacZ转基因小鼠检测二乙基亚硝胺(den)诱发体内遗传毒性。方法　小鼠腹腔注射den(2. 5mg kg) ,每周1次,连续4周。染毒4 8小时后检测外周血微核细胞率。末次染毒7天后处死动物,提取组织DNA ,通过体外包装反应获得并测定肝脏... 详细信息

目的　应用λlacZ转基因小鼠检测二乙基亚硝胺(den)诱发体内遗传毒性。方法　小鼠腹腔注射den(2. 5mg kg) ,每周1次,连续4周。染毒4 8小时后检测外周血微核细胞率。末次染毒7天后处死动物,提取组织DNA ,通过体外包装反应获得并测定肝脏、肺脏、膀胱lacZ基因突变频率。结果　den诱发微核率与对照组无显著性差异,肝脏组织lacZ基因突变(MF)为2 6 8 .4×10 - 6 ,是对照组的6 .13倍,肺脏MF也明显高于对照,是其3 6 6倍,而膀胱组织MF则与对照无显著性差异。结论　肝脏、肺脏均是den致突变的靶器官,但对其敏感度并不相同。

关键词：二乙基亚硝胺转基因小鼠实验研究诱发显著性差异 lacZ 基因突变频率微核细胞率遗传毒性腹腔注射体外包装肝脏组织膀胱组织对照组 den 外周血 DNA 肺脏微核率靶器官致突变敏感度检测染毒 MF

Raccoon Use of den Trees and Plant Associations in Western Mesophytic Forests: Tree Attributes and Availability or Landscape Heterogeneity?

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Natural Resources 2012年第3期3卷 75-87页

作者： Winston P. Smith Keith M. Endres Department of Biology and Center for Management Utilization and Protection of Water Resources Tennessee Technological University Cookeville USA United States Department of Agriculture Forest Service Southern Research Station Center for Bottomland Hardwood Research Stoneville USA Institute of Arctic Biology University of Alaska—Fairbanks Fairbanks USA

We monitored 15 radio-collared raccoons (Procyon lotor) on Davies Island in March 1987 - May 1988 to determine the extent to which individual tree attributes or spatial configuration of plant associations (habitat types) across the landscape influenced den use. Of 1091 verified den sites, 428 were in tree cavities. Raccoon occurrence among 4 cover types differed from that expected based on the total area of each across the island and varied across all seasons for all habitat types except Cedar Wood. Preference varied among age and sex groups and across seasons with some groups showing opposite selection for the same cover type in different seasons. Species and diameter-class distributions of selected den trees differed from a random sample of trees across the landscape. Species composition of trees with cavities also differed from the species composition across the study area. American beech (Fagus grandifolia—relative abundance 8.7%) was over-represented in the sample of trees with cavities (29.4%) and trees selected as dens (65%);diameter at breast height (dbh) of beech den trees averaged 80.0 cm, whereas all beech trees averaged 71.2 cm. For all species combined, mean dbh of den trees was 78.4 cm as compared to trees with cavities (67.6 cm), or all trees (50.4 cm). The relative availability of large, cavity-prone tree species was related to previous logging practices.

关键词： den Use Habitat Use Landscape Heterogeneity Procyon lotor Resource Availability Mesophytic Forest

den2重组E蛋白单克隆抗体的制备及其生物学活性

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中山大学学报（医学科学版） 2005年第1期26卷 56-60页

作者：曾祥凤江丽芳方丹云汤云霞魏惠永晏辉钧中山大学基础医学院微生物学教研室

[目的]研制登革病毒E蛋白特异性单克隆抗体,鉴定其各种生物学特性.[方法]用纯化的Pichia pastoris酵母表达的den2重组E蛋白作为抗原,免疫Balb/c小鼠,采用传统的细胞融合、有限稀释法克隆化杂交瘤细胞,制备稳定分泌McAbs的细胞株.采用硫... 详细信息

[目的]研制登革病毒E蛋白特异性单克隆抗体,鉴定其各种生物学特性.[方法]用纯化的Pichia pastoris酵母表达的den2重组E蛋白作为抗原,免疫Balb/c小鼠,采用传统的细胞融合、有限稀释法克隆化杂交瘤细胞,制备稳定分泌McAbs的细胞株.采用硫酸铵沉淀法纯化腹水中的McAbs,用ELISA、IFA、Western Blot和空斑减数中和实验等鉴定McAbs的各种生物学活性.[结果]用ELISA、IFA检测证实5株杂交瘤细胞产生的McAbs与den2重组E蛋白和den全病毒均有较高的亲和力;Western Blot分析发现其中3株杂交瘤细胞(3G3,6G11,4E3)分泌的McAbs能与其相对分子质量Mr=(66～69)×103的den2重组E蛋白结合.空斑减数中和实验证实所获得的单克隆抗体具有中和登革病毒感染C6/36细胞的作用.[结论]成功地制备了5株抗登革病毒E蛋白特异性的McAbs,为进一步研究E蛋白的结构和功能及临床诊断试剂盒研发打下了基础.

关键词： E蛋白 den 单克隆抗体生物学活性登革病毒 ELISA 分泌杂交瘤细胞硫酸铵沉淀法纯化

A novel two-phase carcinogenesis method using den as an inducer in Fischer rats

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A novel two-phase carcinogenesis method using DEN as an indu...

China Cultural Center in den Haag:Achieving Accessibility-Oriented Communication and Creating Shared Space

第三届中日双边药理学和临床药理学学术会议

作者： ZHANG Lin LI Bo SHEN Lian-Zhon (National Institute for the Control of Pharmaceutical and Biological Products-National Center for the Evaluation of Drugs,Beijing 100176,China)

AIM To investigate a novel two-phase carci- nogenesis method using den as an inducer in Fischer *** Sixty male and 60 female 5-week Fis- cher rats were randomized into 3 groups:NS single intrap- eritoneal injection group,200mg·kgden single intrap- eritoneal injection group and 200mg·kgden single in- traperitoneal injection +10ppm den administration via drinking water *** d 1,200mg·kgden was in- jected to the animals according to their body weights to ini- tiate the *** d 2 onwards,10ppm den was administered daily to the animals via drinking water to boost the carcinogenesis for 3 *** The organs were histopathologically evaluated by HE staining. After initiated by single intraperitoneal injection of 200mg ·kgden,the livers of the animals proliferated 40% for females and 70% for *** boosted by 10 ppm den administration via drinking water,for males,the liv- er proliferated 100% with 30% tumorous node and 10% tumor;for females,the liver proliferated 100% with 30% tumorous node and 5% *** Single intraperitoneal injection of 200mg·kgden induced pre- malignant lesions in a small quantity of liver ***- quent administration of 10 ppm den via drinking water in- duced pre-malignant lesions in a large quantity of liver cell and liver *** two-phase method is a promising no- vel method to be used in carcinogenesis test to evaluate the carcinogenesis of the chemicals.

关键词： den two-phase method carcinogenesis

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China & The World Cultural Exchange 2020年第10期86卷 36-37页

作者： Zhang Qing Zhang Meilun 不详

Given that offline events are currently restrained by the COVID-19 outbreak,China Cultural Center(CCC)in den Haag redesigned its strategy for China-Netherlands people-to-people ties to focus on online events,taking innovative measures to achieve accessibility-oriented communication and create shared space for cultural and tourism exchanges.

关键词： restrained den innovative

肝郁脾虚因素刺激对den诱发肝癌大鼠免疫功能的影响

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北京中医药大学学报 2002年第6期25卷 34-37页

作者：彭桂英顾立刚王庆国王济李瑞生王秀茜北京中医药大学微生物与免疫教研室北京100029

观察肝郁脾虚因素刺激对二乙基亚硝胺 (den)诱发大鼠实验性肝癌的发生及T淋巴细胞功能的影响 ,以及用疏肝健脾方药对该模型的防治作用。大鼠经肝郁脾虚因素刺激和den 70mg/kg灌胃 ,每周 1次 ,连续 8周后停用den ,预防和治疗组给予疏肝... 详细信息

观察肝郁脾虚因素刺激对二乙基亚硝胺 (den)诱发大鼠实验性肝癌的发生及T淋巴细胞功能的影响 ,以及用疏肝健脾方药对该模型的防治作用。大鼠经肝郁脾虚因素刺激和den 70mg/kg灌胃 ,每周 1次 ,连续 8周后停用den ,预防和治疗组给予疏肝健脾方药 2mL/只灌胃 (1 5g/kg)。大鼠分别在实验第 9、14、19周取脾测定T淋巴细胞增殖和产生IFN γ和IL 2的能力。结果显示 :大鼠经肝郁脾虚因素刺激和den作用下 ,与单纯den诱发肝癌大鼠比较 ,T淋巴细胞增殖和产生IFN γ和IL 2能力的显著降低 (P值分别 den诱发肝癌大鼠的免疫功能。

关键词：肝郁脾虚 den 诱发肝癌大鼠免疫功能 T淋巴细胞疏肝健脾方

den诱发SD大鼠原发性肝癌形成过程中AFU价值的研究

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陕西医学杂志 2005年第5期34卷 532-533,537页

作者：何谦刘余静赵彩蕾西安交通大学第二医院检验科西安710004

目的总结探讨a-L-岩藻糖苷酶(AFU)对原发性肝癌的早期诊断价值。方法将65只SD雄性大鼠随机分为诱癌组和对照组,两组分别于第6,8,11,14,17,20周行尾静脉采血进行血清学指标检查,同时进行MR平扫及其他影像学检查和病理检查。结果AFP直到... 详细信息

目的总结探讨a-L-岩藻糖苷酶(AFU)对原发性肝癌的早期诊断价值。方法将65只SD雄性大鼠随机分为诱癌组和对照组,两组分别于第6,8,11,14,17,20周行尾静脉采血进行血清学指标检查,同时进行MR平扫及其他影像学检查和病理检查。结果AFP直到第18周肝癌演变期与正常对照组无显著性差异;AFU从第14周早期肝癌形成时就与正常对照组有显著性差异,并且随着肝癌的动态发展过程进一步升高。结论AFU对原发性肝癌具有较高的早期诊断价值。

关键词：原发性肝癌 AFU 形成过程 SD大鼠 den 早期诊断价值显著性差异正常对照组诱发 SD雄性大鼠岩藻糖苷酶血清学指标影像学检查静脉采血病理检查 MR平扫同时进行早期肝癌 AFP