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dek42 encodes an RNA-binding protein that affects alternative pre-m RNA splicing and maize kernel development

Journal of Integrative Plant Biology 2019年第6期61卷 728-748页

作者： Yi Zuo Fan Feng Weiwei Qi Rentao Song State Key Laboratory of Plant Physiology and Biochemistry National Maize Improvement CenterBeijing Key Laboratory of Crop Genetic ImprovementJoint International Research Laboratory of Crop Molecular BreedingCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Shanghai Key Laboratory of Bio-Energy Crops Plant Science CenterSchool of Life SciencesShanghai UniversityShanghai 200444China

RNA-binding proteins (RBPs) play an important role in post-transcriptional gene regulation. However, the functions of RBPs in plants remain poorly understood. Maize kernel mutant dek42 has small defective kernels and lethal seedlings. dek42 was cloned by Mutator tag isolation and further confirmed by an independent mutant allele and clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 materials. dek42 encodes an RRM_RBM48 type RNA-binding protein that localizes to the nucleus. dek42 is constitutively expressed in various maize tissues. The dek42 mutation caused a significant reduction in the accumulation of dek42 protein in mutant kernels. RNA-seq analysis showed that the dek42 mutation significantly disturbed the expression of thousands of genes during maize kernel development. Sequence analysis also showed that the dek42 mutation significantly changed alternative splicing in expressed genes, which were especially enriched for the U12-type intron-retained type. Yeast two-hybrid screening identified SF3a1 as a dek42-interacting protein. dek42 also interacts with the spliceosome component U1-70K. These results suggested that dek42 participates in the regulation of pre-messenger RNA splicing through its interaction with other spliceosome components. This study showed the function of a newly identified RBP and provided insights into alternative splicing regulation during maize kernel development.

关键词： dek42 encodes an RNA-binding protein that affects alternative pre-mRNA splicing and maize kernel development^FA dek42 encodes

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