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Dynamic deformylation of 5-formylcytosine and decarboxylation of 5-carboxylcytosine during differentiation of mouse embryonic stem cells into mouse neurons

引用

Chinese Chemical Letters 2024年第11期35卷 278-283页

作者： Yang Feng Yang-Qing Tian Yong-Qiang Zhao Sheng-Jun Chen Bi-Feng Yuan Key Laboratory of Aquatic Product Processing Ministry of Agriculture and Rural AffairsNational R&D Center for Aquatic Product ProcessingSouth China Sea Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhou 510300China Department of Occupational and Environmental Health School of Public HealthResearch Center of Public HealthRenmin Hospital of Wuhan UniversityWuhan UniversityWuhan 430071China College of Veterinary Medicine Yunnan Agricultural UniversityKunming 650000China

Regulation of cell fate requires the establishment and erasure of 5-methylcytosine(5mC) in genomic *** formation of 5mC is achieved by DNA cytosine methyltransferases(DNMTs),whereas the removal of5mC can be accomplished by various *** from ten-eleven translocation(TET)-mediated oxidation of 5mC followed by thymine DNA glycosylase(TDG)-initiated base excision repair(BER),the direct deformylation of 5-formylcytosine(5fC) and decarboxylation of 5-carboxylcytosine(5caC) have also been discovered as the novel DNA demethylation *** these novel demethylation pathways have been identified in stem cells and somatic cells,their precise roles in regulating cell fate remain ***,we differentiate mouse embryonic stem cells(mESCs) into mouse embryoid bodies(mEBs),followed by further differentiation into mouse neural stem cells(mNSCs) and finally into mouse neurons(mNeurons).During this sequential differentiation process,we employ probe molecules,namely2'-fluorinated 5-formylcytidine(F-5fC) and 2'-fluorinated 5-carboxyldeoxycytidine(F-5caC),for metabolic *** results of mass spectrometry(MS) analysis demonstrate the deformylation and decarboxylation activities are progressively decreased and increased respectively during differentiation process,and this opposite demethylation tendency is not associated with DNMTs and TETs.

关键词： Metabolic labeling Mass spectrometry DNA demethylation deformylation Decarboxylation C-C cleavage Mouse embryonic stem cells Differentiation

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