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Microorganisms from deep-sea hydrothermal vents

Marine Life Science & Technology 2021年第2期3卷 204-230页

作者： Xiang Zeng Karine Alain Zongze Shao Key Laboratory of Marine Biogenetic Resources Third Institute of OceanographyMinistry of Natural ResourcesXiamen361005China Laboratoire de Microbiologie des Environnements Extrêmes LM2E UMR6197 Univ BrestCNRSIFREMERF-29280PlouzanéFrance LIA/IRP 1211 Microbsea Sino-French International Laboratory of Deep-Sea Microbiology29280PlouzanéFrance

With a rich variety of chemical energy sources and steep physical and chemical gradients,hydrothermal vent systems offer a range of habitats to support microbial ***-dependent and independent studies have led to an emerging view that diverse microorganisms in deep-sea hydrothermal vents live their chemolithoautotrophic,heterotrophic,or mixotrophic life with versatile metabolic *** processes are mediated by microorganisms,and notably,processes involving or coupling the carbon,sulfur,hydrogen,nitrogen,and metal cycles in these unique ***,we review the taxonomic and physiological diversity of microbial prokaryotic life from cosmopolitan to endemic taxa and emphasize their significant roles in the biogeochemical processes in deep-sea hydrothermal *** to the physiology of the targeted taxa and their needs inferred from meta-omics data,the media for selective cultivation can be designed with a wide range of physicochemical conditions such as temperature,pH,hydrostatic pressure,electron donors and acceptors,carbon sources,nitrogen sources,and growth *** application of novel cultivation techniques with real-time monitoring of microbial diversity and metabolic substrates and products are also recommended.

关键词： deep-sea hydrothermal vents Cultivation Diversity Biogeochemical cycle

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Identification and Characterization of a Novel Alkalistable and Salt-Tolerant Esterase from deep-sea hydrothermal vents

Identification and Characterization of a Novel Alkalistable ...

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华东地区生物化学与分子生物学学会联合会2017年学术交流会暨安徽省生物化学与分子生物学学会理事会议

作者： Xinwei Yang Lianzuan Wu Ying Xu Chongrong Ke Fangfang Hu Songgang Wu Jianzhong Huang Engineering Research Center of Industrial Microbiology Ministry of EducationFujian Normal University

A novel esterase gene that was selected from a metagenomic dataset of a deep-sea hydrothermal vent was successfully expressed in Escherichia *** recombinant protein(est-OKK),which belongs to the lipase family V,exhibited high activity toward pNP-esters with short acyl chains and especially p-nitrophenyl *** apparent K and V values for reactions with p-nitrophenyl butyrate were 483 μM and 161.3 U/mg,*** est-OKK protein is a moderately thermophilic enzyme that is relatively thermostable and highly salt-tolerant and remained stable in 3 M NaCI for 6 *** est-OKK protein showed the considerable alkalistability,displayed optimal activity at pH 9.0 and maintained approximately 70%of its residual activity after incubation at pH 10 for 4 ***,est-OKK was strongly resistant to Co,Zn,Fe,Na,EDTA,and organic solvents such as acetone and *** together,these results suggest that this esterase could be a highly valuable candidate for biotechnological applications such as organic synthesis reactions and those found in the laundry industry.

关键词： Esterase Alkalistable enzyme Salt-tolerant enzyme deep-sea hydrothermal vents

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