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Activation of Ca2+ -activated chloride current by membrane depolarization in rat drg neurons

Activation of Ca2+ -activated chloride current by membrane d...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Xuan ZHANG,Hai-Lin ZHANG~* Department of Pharmacology,Hebei Medical University,Shijiazhuang 050017,China

Ca-activate chloride current(CACC) plays important physiological roles in different tissues including neuronal *** is not clear whether there is specificity in CACC activation by Ca from different sources,namely Ca influx throμgh voltage-/ligand-gated Ca channels in the plasma membrane,vs Ca release from intracellular *** this study we investigated the activation of CACC by intracellular Ca induced by the membrane depolarization in rat drg *** whole-cell patch recordings were performed on drg neurons from 7-dayold SD *** the condition of regular external(high[NaCl]) and internal(high[KCl]) solution,brief depolarization evoked a large inward tail current at -80 *** depolarization-induced tail current selectively appeared in a subset of medium-diameter drg neurons and could be partially blocked by chloride current inhibiters DIDS and NFA, indicating a possibly component of *** tail current could also be blocked by Ca free external solution, pretreatment with BAPTA-AM,and NiCl,indicating Ca influx is essential for the activation of *** fura- 2AM-loaded neurons,brief depolarization range from -30 to +90 mV induced a depolarization-dependent increase of intracellular *** it seems that the depolarization-induced CACC activation and intracellular Ca increase is not merely a result of opening of voltage-dependent Ca *** Na and internal K also affected the depolarization-induced CACC activation and intracellular Ca *** the membrane depolarization-induced activation of CACC may use intracellular Ca throμgh Ca channels as well as transporters.

关键词： calcium drg neuron Ca2+-activate chloride current membrane depolarization voltage-dependent Ca2+ channel

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BmK I directly activates Nav1.8 in primary sensory neurons to induce neuronal hyperexcitability in rats

BmK I directly activates Nav1.8 in primary sensory neurons t...

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第12届生物毒素研究及医药应用学术大会

作者： Pin Ye Li-Ming Hua Yong-Hua Ji Laboratory of Neuropharmacology and Neurotoxicology Shanghai University

Voltage-gated sodium channels(VGSCs) in primary sensory neurons play a key role in transmitting pain signals to the central nervous *** I,a site-3 sodium channel-specific toxin from scorpion Buthus martensi Karsch,induces pain behaviors in ***,the subtypes of VGSCs targeted by BmK I were not entirely *** therefore investigated the effect of BmK I on the current and kinetic properties of Na1.8,which is associated with neuronal hyperexcitability in drg *** was found that BmK I dose-dependently increase Na1.8 current in small-sized(drg neurons,which correlated with its inhibition on both fast and slow ***,voltage-dependent activation and steady-state inactivation curves of Na1.8were shifted in a hyperpolarized ***,BmK I reduced the threshold of neuronal excitability and increased action potential firing in drg *** conclusion,the data fruited in the study clearly demonstrated that BmK I modulated Na1.8remarkably,suggesting BmK I as a valuable probe for studying Na1.8,and Na1.8 is an important target related to BmK I-evoked pain.

关键词： BmK I drg neuron Nav1.8 Hyperexcitability

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IPP5 inhibits neurite growth in primary sensory neurons by main-taining TGF-β/Smad signaling

IPP5 inhibits neurite growth in primary sensory neurons by m...

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中国神经科学学会第十届全国学术会议

作者： Qing-Jian Han Nan-Nan Gao Guo-Qiang Ma Zhen-Ning Zhang Wen-Hui Yu Jing Pan Qiong Wang Xu Zhang Lan Bao State Key Laboratory of Cell Biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of Sciences Institute of Neuroscience and State Key Laboratory of Neuroscience Shanghai Institutes for Biological SciencesChinese Academy of Sciences

Objective Neurite growth is a critical step for successful nerve regeneration after injury and it is regulated by both intrinsic molecules and extracellular *** found that inhibitor 5 of protein phosphatase 1(IPP5),a newly identified inhibitory subunit of protein phosphatase 1(PP1),was highly expressed in rat primary sensory neurons localized in the dorsal root ganglion(drg),specifically in small primary sensory neurons,and was downregulated after sciatic nerve axotomy,and may serve as a neurite growth inhibitory molecule in primary sensory *** we explore the regulatory functions of IPP5 in drg neurite ***(1) Knocking down IPP5 with specific shRNA and examining the neurite growth of cultured drg neurons;(2) Mutating the PP1 binding motif or the PP1inhibiting motif at Thr to determine whether the inhibition of PP1 is responsible for the effect of IPP5 on neurite growth;(3) Blocking TGF-p signaling with TβRI inhibitor SB431542 or Smad2 shRNA to clarify the involvement of TGF-p signaling ***(1) Knocking down IPP5 promote neurite growth in cultured primary sensory neurons.(2) The interaction with and inhibition of PP1 is responsible for the effect of IPP5 on neurite growth.(3) IPP5inhibits neurite growth by regulating the TGF-p signaling *** IPP5 inhibits neurite growth in primary sensory neurons in a PP1-dependent manner and functions by maintaining TGF-p/Smad signaling pathway.

关键词： IPP5 neurite growth PP1 TGF-β/Smad signaling drg neuron

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