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Addressable dna Information Processing System with a Fluorescent Readout for Rewritable Memory

Chinese Journal of Chemistry 2023年第20期41卷 2628-2634页

作者： Zirui Xi Mengyao Yang Yuqiang Hu Tongbo Wu School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei430030 China

dna-based nanostructure allows the construction of molecular devices useful in biological computing and information ***,an addressable and editable dna information processing system established on a fluorescence intensity signal detection platform to save and encrypt information is *** system operates by encoding information into distinct and changeable units of the trigger strands decoding by fluorescence intensity signal *** toehold-mediated strand displacement reactions,the trigger strand can be precisely added to and removed from the memorizer and reporter to implement the function of editing,encrypting,and *** strategy is simple to implement,requiring only two mixing steps at room temperature for each operation and fluorescence intensity signal detection to read the *** the system can realize accurate retrieval of specific individual information,eliminating all unnecessary redundant *** of its point-to-point accurate readout and programmability,the system is expected to become a powerful tool for the future development of information storage and sensing of biological molecules.

关键词： Information processing Strand displacement Self-assembly Fluorescence dna structures

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Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers

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Chinese Physics B 2018年第6期27卷 543-553页

作者： Yi-Zhou Wang Xi-Miao Hou Hai-Peng Ju Xue Xiao Xu-Guang Xi Shuo-Xing Dou Peng-Ye Wang Wei Li Key Laboratory of Soft Matter Physics Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100190 China School of Life Sciences North west A ＆ F University Yangling 712100 China School of Physical Sciences University of Chinese Academy of Sciences Beijing 100049 China

Human telomeric G-quadruplex plays a crucial role in regulating the genome stability. Despite extensive studies on structures and kinetics of monomeric G-quadruplex, the interaction between G-quadruplexes is still in debate. In this work,we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K~＋buffer. The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed. The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes. Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.

关键词： biophysics G-quadruplexes single-molecule studies dna structures

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dna-Based Architectures for in situ Target Biomolecule Analysis in Confined Nano-space

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Chinese Journal of Chemistry 2021年第7期39卷 2027-2034页

作者： Xiaoxue Hu Yide Huang Hao Yin Lizhi Dai Ye Tian Shenzhen Research Institute Nanjing UniversityShenzhenGuangdong518000 China College of Engineering and Applied Sciences State Key Laboratory of Analytical Chemistry for Life ScienceChemistry and Biomedicine Innovation CenterNanjing UniversityNanjingJiangsu210093 China

In situ target biomolecule analysis is of great significance for real-time monitoring and regulation of endogenous biomarkers and elementary biomolecules in ***,the rapid evolution of structural dna nanotechnology during past decades has established an appealing toolbox for biological analysis and medical *** modulated self-assembly and underlying canonical Watson-Crick base-pairing rules provide possibilities for accurate controlling of the topologies and functions of obtained *** probes composed of diverse dna nanostructures and dna-nanoparticle complexes can create a confined space,which increases target accessibility and improves probe stability,sensitivity and *** this minireview,we retrospect the research progress of in-situ biomolecular analysis based on dna nanostructures for intracellular and in vivo biosensors in confined *** characteristics of distinct dna nanomaterials are first introduced,and then the fundamentals of biosensing process of designed dna nanostructures are ***,we elucidate our perspective over the challenges of this field and discuss the potential directions of this kind of application-oriented fabrication technique.

关键词： Nanotechnology Biomolecule analysis dna structures Fluorescent probes Confined nanospace

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Versatile Magnetic Nanoparticles for Spatially Organized Assemblies of Enzyme Cascades:A Comprehensive Investigation of Catalytic Performance

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Chinese Journal of Chemistry 2022年第12期40卷 1437-1446页

作者： Xinshuang Chu Qinghong Shi Deportment of Biochemical Engineering School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350China Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology(Ministry of Education) Tianjin UniversityTianjin 300350China

Inspired by nature,precise spatial organization of enzyme cascades of interest is crucial to the improvement of catalytic ***,dna scaffolds were introduced to construct a toolkit for versatile immobilization of enzyme pairs on dextran-coated magnetic nanoparticles(MNPs).After the glucose oxidase(GOx)and horseradish peroxidase(HRP)pair was immobilized through random cova-lent,dna-directed and dna tile-directed strategies,the immobilized GOx/HRP pair on the MNP-based carrier assembled with dna tile(TD@MNPs)exhibited the highest activity due to rational spatial organization and less conformational change of constituent *** a decrease in interenzyme distance on TD@MNPs,furthermore,the catalytic efficiency of the HRP/GOx pair increased further for both substrates,2,2'-azinobis(3-ethyl-benzthiazoline-6-sulfonate)(ABTS)and 3,3',5,5'-tetramethyl benzidine(TMB).As the assembled HRP was closer to the carrier surface,the catalytic efficiency of the GOx/HRP pair increased by 6.2-fold for positively charged TMB and only by 62%for negatively charged ABTS compared with the free GOx/HRP ***,a reversal of catalytic efficiency was found after the GOx/HRP pair was assembled on a positively charged carrier(TD@pMNPs).This research demonstrated that MNP-based car-riers had the potential to become a versatile toolkit for shedding an insight into catalytic performance and the development of new biocatalysts.

关键词： Enzymes Immobilization dna structures Substrate transport Spatial organization

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