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Molecular characterization and subtyping of Blastocystis in urticarial patients in Turkey

Asian Pacific Journal of Tropical Medicine 2019年第10期12卷 450-456页

作者： Merve Aydin Mustafa Yazici Mehtap Demirkazik Ismail Soner Koltas Aytekin Cikman Baris Gulhan Tugce Duran Aysun Yilmaz Murat Kara Department of Medical Microbiology Faculty of MedicineErzincan UniversityErzincanTurkey Department of Medical Microbiology Faculty of MedicineKTO Karatay UniversityKonyaTurkey Department of Dermatology Faculty of MedicineErzincan UniversityErzincanTurkey Department of Medical Parasitology Faculty of MedicineCukurova UniversityAdanaTurkey Department of Medical Genetics Faculty of MedicineKTO Karatay UniversityKonyaTurkey Department of Medical Genetics and Molecular Biology Institute of Health SciencesKocaeli UniversityKocaeliTurkey Department of Medical Microbiology Institute of Health SciencesErzincan UniversityErzincanTurkey

Objective:To investigate Blastocystis’etiologic role and association with gastrointestinal symptomatology in acute and chronic urticaria patients and to identify Blastocystis subtypes responsible for ***:The study included urticaria patients and healthy individuals that presented to our polyclinic between June 2015 and May *** participants were assigned into Group栺(137 patients),subdivided into acute(72)and chronic urticaria patients(65),and Group栻(129 control individuals).Blastocystis presence was investigated by native-Lugol examination,trichrome staining,PCR using sequence tagged site primers,and dna sequencing *** phylogenetic tree was ***:The native-Lugol and trichrome staining methods revealed that 16 patients(16/133,12.0%)had Blastocystis-positive stool samples,of which seven samples(7/133,5.3%)belonged acute and nine(9/133,6.8%)to chronic urticaria *** Blastocystis subtypes,of the acute urticaria patients,three had subtype 1(ST1),one had ST2,and three had *** the chronic urticaria patients,one had ST1 and eight had *** positivity was detected in two control individuals(2/123,1.6%),both being *** subtypes identified by PCR were confirmed by the sequencing *** acute and chronic urticaria groups showed no statistically significant differences for Blastocystis positivity(P=0.60)and subtype distribution(P=0.15).A statistically significant difference was found between the urticaria patients and the controls for Blastocystis positivity(P<0.01),but not for subtype distribution(P=0.67)or for Blastocystis presence and gastrointestinal ***:This study on Blastocystis subtype distribution among Turkish urticaria patients showed results consistent with the *** was concluded that Blastocystis should be kept in mind in patients with urticaria.

关键词： Urticaria Blastocystis Subtypes PCR dna sequence analysis

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Isolation and Characterization of Microsatellites in Snap Bean

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Acta Botanica Sinica 2000年第11期42卷 1179-1183页

作者：郭建春胡新文柳原诚司江川宜伸中国热带农业科学院热带作物生物技术国家重点实验室海口571101 日本国际农业科学研究中心

The objectives of this study were to isolate and characterize microsatellites from a heat tolerant variety of snap bean (Phaseolus vulgaris L.) in order to generate polymorphic genetic markers linked to quantitative trait loci for heat tolerance. A genomic library contained 400-800 bp inserts was constructed and screened for the presence of (GA/CT) n and (CA/GT) n repeats. The proportion of positive clones yielded estimated of 3.72×10 4 such dinucleotide repeats per genome, roughly comparable to the abundance reported in other eukaryotic genomes. Twenty_six positive clones were sequenced. In contrast to mammalian genomes, the (GA/CT) n motif was much more abundant than the (CA/GT) n motif in these clones. The (GA/CT) n repeats also showed longer average repeat length (mean n =10.4 versus 6.5), suggesting that they are better candidates for yielding polymorphic genetic markers in the snap bean genome.

关键词： Phaseolus vulgaris genomic library microsatellite molecular marker dna sequence analysis

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The updated phylogenetic lineages and species recognized from the genus Tuber in China

The updated phylogenetic lineages and species recognized fro...

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中国菌物学会2015年学术年会

作者： Li Fan College of life science Capital normal university

According to the species delimitation of 95% sequence matches in the ITS region, we recognized 58 phylogenetic Tuber species in China（excluding the two previously described species T. xizangense and T. gigantosporum, because their dna data are absent）. These species could be grouped into 9 distinct phylogenetic lineages, including an new lineage Latisporum that is distributed in Asia only, based on the phylogenetic analyses with sequences of four nuclear loci（ITS, LSU, EF1-a, and rpb2 genes）. These results indicated that China is rich in both species diversity and evolutionary phylogeny of this economically and ecologically important truffle group in the world. These lineages and species of Tuber in China are listed as follows: 1. Lineage Aestivum T. sinoaestivum 2. Lineage Excavatum T. neoexcavatum-A T. neoexcavatum-B T. neoexcavatum-C T. sinoexcavatum T. pseudofulgens（nom. prov.） 3. Lineage Japonicum T. turmericum（nom. prov.） T. sp.2（ECM-Zhejiang province） T. sp.3（ECM-Zhejiang province） T. sp.14（ECM-Sichuan province） 4. Lineage Latisporum T. alboumbilicum T. caoi T. latisporum T. parvomurphium T. pseudospherosporum T. baoshanense（nom. prov.） T. daliense（nom. prov.） T. huiliense（nom. prov.） T. linzhiense（nom. prov.） T. muliense（nom. prov.） T. neosphaerosporum（nom. prov.） T. sp.4（ECM-Heilongjiang province）T. sp.5（ECM-Guangdong province） T. sp.6（ECM-Hunan province） T. sp.7（DQ478639/IFS89261, Gansu Province） T. sp.8（DQ478623/IFS89196, Gansu Province） ***.9（DQ478624/IFS89556, Liaoning Province） T. sp.10（ECM-North China） 5. Lineage Macrosporum T. glabrum T. sinomonosporum T. neoglabrum（nom. prov.） 6. Lineage Maculatum T. bomiense T. hubeiense T. pseudomagnatum T. wumengense T. sp.1（ECM-Shannxi and Heilongjiang） 7. Lineage Melanosporum T. pseudobrumale T. pseudohimalayense T. sinense-A T. sinense-B T. sp.13（GU979047, Taiwan） 8. Lineage Puberulum T. jinshajiangense T. lijiangense T. liui T. sinosphaerosporum T. xuanhuaense T. shii（nom. prov.） T. sp.11（ECM-Zhejiang provin

关键词： ascomycota dna sequence analysis phylogeny

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