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Electrochemical sensing of dna immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film

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Chinese Chemical Letters 2008年第5期19卷 589-591页

作者： Wei Zhang Tao Yang Da Ming Huang Kui Jiao College of Chemistry and Molecular Engineering Qingdao University of Science and Technology Qingdao 266042 China

A novel electrochemical dna biosensor based on zinc oxide （ZnO） nanoparticles and multi-walled carbon nanotubes （MWNTs） for dna immobilization and enhanced hybridization detection is presented. The MWNTs/nano ZnO/chitosan composite film modified glassy carbon electrode （MWNTs/ZnO/CHIT/GCE） was fabricated and dna probes were immobilized on the electrode surface. The hybridization events were monitored by differential pulse voltammetry （DPV） using methylene blue （MB） as an indicator. The sensor can effectively discriminate different dna sequences related to PAT gene in the transgenic corn, with a detection limit of 2.8× 10^-12 mol/L of target sequence.

关键词： ZnO nanoparticles Multi-walled carbon nanotubes Chitosan dna sensor PAT gene

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The roles of interferon-inducible p200 family members IFI16 and p204 in innate immune responses,cell differentiation and proliferation

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Genes & Diseases 2015年第1期2卷 46-56页

作者： Hua Zhao Elena Gonzalezgugel Lei Cheng Brendon Richbourgh Lin Nie Chuanju Liu Department of Orthopaedic Surgery New York University School of MedicineNew YorkNY 10003United States Department of Spine Surgery Qilu Hospital of Shandong UniversityJinan250014China Department of Cell Biology New York University School of MedicineNew YorkNY 10016United States

p204 is a member of the interferon-inducible p200 family proteins in *** p200 family has been reported to be multifunctional regulators of cell proliferation,differentiation,apoptosis and ***-inducible protein 16(IFI16)is regarded as the human ortholog of p204 in several *** is possibly due to the similarity of their *** the consistency of their functions is still ***,an emerging focus has been placed upon the role of the p200 proteins as sensors for microbial dna in innate immune responses and provides new insights into infections as well as autoimmune *** review specially focuses on IFI16 and p204,the member of p200 family in human and murine respectively,and their pathophysiological roles in innate immune responses,cell differentiation and proliferation.

关键词： dna sensor IFI16 Innate immune response Multifunctional regulator p204

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