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第36届中国控制会议

作者： Tingting Huang Zhengxun Song Ying Wang Feifei Wang Fenfen Guo Zuobin Wang International Research Centre for Nano Handling and Manufacturing of China Changchun University of Science and Technology

ISBN: (纸本)9781538629185

The force acting between molecules and substrates is crucial for the manipulation *** force microscopy（AFM） is not only a versatile tool for imaging in biological research,but also a versatile one for the manipulation of biological *** of the high mechanical resolution of AFM,it is preferred for the study of the interaction of mechanics in biological *** work is focused on the using of the Single-molecule force spectroscopy based on AFM（AFM-SMFS） method in the process of dna *** explored the manipulation methods based on AFM,and then introduced one of the modification methods of the dna molecules and the AFM *** with the different statements of the AFM cantilever by AFM-SMFS,analyzed the adhesion force between the molecules and the substrates.

关键词： Nano-manipulation AFM dna manipulation AFM-SMFS

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Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第2期17卷 83-99页

作者： Jian-zhong XU Wei-guo ZHANG The Key Laboratory of Industrial Biotechnology Ministry of Education School of Biotechnology Jiangnan University

With the availability of the whole genome sequence of Escherichia coli or Corynebacterium glutamicum, strategies for directed dna manipulation have developed rapidly. dna manipulation plays an important role in understanding the function of genes and in constructing novel engineering bacteria according to requirement. dna manipulation involves modifying the autologous genes and expressing the heterogenous genes. Two alternative approaches, using electroporation linear dna or recombinant suicide plasmid, allow a wide variety of dna manipulation. However, the over-expression of the desired gene is generally executed via plasmid-mediation. The current review summarizes the common strategies used for genetically modifying E. coli and C. glutamicum genomes, and discusses the technical problem of multi-layered dna manipulation. Strategies for gene over-expression via integrating into genome are proposed. This review is intended to be an accessible introduction to dna manipulation within the bacterial genome for novices and a source of the latest experimental information for experienced investigators.

关键词： Escherichia coli Corynebacterium glutamicum dna manipulation Site-directed mutagenesis Gene inactivation Gene over-expression

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