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Target-triggered hairpin-free chain-branching growth of dna dendrimers for contrast-enhanced imaging in living cells by avoiding signal dispersion

引用

Chinese Chemical Letters 2022年第2期33卷 773-777页

作者： Jiaqi Deng Jingyuan Xu Minzhi Ouyang Zhen Zou Yanli Lei Junbin Li Zhihe Qing Ronghua Yang Hunan Provincial Key Laboratory of Cytochemistry School of Chemistry and Food EngineeringChangsha University of Science and TechnologyChangsha 410114China Laboratory of Chemical Biology&Traditional Chinese Medicine Research Ministry of EducationCollege of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha 410081China Department Ultrasound Diagnosis The Second Xiangya HospitalCentral South UniversityChangsha 410011China

The development of amplification strategies is one of the central challenges for detection of lowabundance targets. One-to-many(1:M) amplification strategies in which one target lights many signal probes, has improved the detection sensitivity in bulk solution, but with discounted contrast in cell imaging, because the lighted probes are dissociative and dispersible. In this work, a one-to-large(1:L) signaling mechanism, in which the lighted probes were orderly connected to each other, was conceptually proposed to enhance the contrast in cell imaging by avoiding signal dispersion in amplification. Accordingly,target-triggered hairpin-free chain-branching assembly(HFCBA) holds great potential to implement the1:L mechanism, but using it in cell imaging has yet to be demonstrated. As a proof of concept, a group of probes were first programmed to implement mi RNA-21-triggered HFCBA. After transfection of probes,gradually-growing signal flares in cells were monitored along with the growth of dna dendrimers;and the in situ fluorescence accumulation in HFCBA resulted in highly-enhanced contrast to the surrounding by avoiding signal dispersion in amplification. The contrast-enhanced imaging with signal amplification is significant for biological analysis and molecular medicine. We expect the 1:L mechanism will provide a new thought for high-performance imaging of biomarkers in cells.

关键词： Cell imaging Amplification Signal dispersion Self-assembly dna dendrimers

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