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Synthesis and dna adsorption of Poly(2-Vinyl-4,6-Diamino-1,3,5-Triazine)Coated Polystyrene Microspheres

Journal of Wuhan University of Technology(Materials Science) 2018年第4期33卷 999-1006页

作者：黄雷王小涛 XIE Xun XIE Weihong LI Xuefeng GONG Xinghou LONG Shijun GUO Huiling 刘最芳 Hubei Provincial Key Laboratory of Green Materials for Light Industry School of Materials and Engineering Hubei University of Technology Collaborative Innovation Center of Green Light-weight Materials and Processing Hubei University of Technology School of Food and Biological Engineering Hubei University of Technology

To take advantage of the polymer＇s special properties, we synthesized PVDAT coated polystyrene（PVDAT@PS） microspheres through semi-continuous precipitation polymerization, and demonstrated the possibility of controlling the size of microspheres by using different sized PS cores. Mechanism of the microsphere synthesis was investigated. Preliminary application studies indicated that the synthesized microspheres adsorbed double-stranded dnas（dsdnas） comprising A-T base pairs in pH7.4 phosphate buffered saline, and the adsorption capacity was in a range of 3.28 mg to 5.58 mg per gram PVDAT@PS microspheres depending on A-T base pair percentage and chain length of the dsdnas.

关键词： 2-vinyl-4,6-diamino-l,3,5-triazine PVDAT@PS dna adsorption semi-continuousprecipitation polymerization

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Clamped-end effect on static detection signals of dna-microcantilever

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Applied Mathematics and Mechanics(English Edition) 2021年第10期42卷 1423-1438页

作者： Junzheng WU Nenghui ZHANG Shanghai Institute of Applied Mathematics and Mechanics Shanghai Key Laboratory of Mechanics in Energy EngineeringSchool of Mechanics and Engineering ScienceShanghai UniversityShanghai 200072China

Boundary constraint induced inhomogeneous effects are important for mechanical responses of nano/*** microcantilever sensors,the clamped-end constraint induced inhomogeneous effect of static deformation,so called the clamped-end effect,has great influence on the detection *** paper is devoted to developing an alternative mechanical model to characterize the clamped-end effect on the static detection signals of the *** from the previous concentrated load models,the dna adsorption is taken as an equivalent uniformly distributed tangential load on the substrate upper surface,which exactly satisfies the zero force boundary condition at the ***,a variable coefficient differential governing equation describing the non-uniform deformation of the dna-microcantilever induced by the clamped-end constraint is established by using the principle of minimum potential *** reducing the order of the governing equation,the analytical solutions of the curvature distribution and static bending deflection are *** comparing with the previous approximate surface stress models,the clamped-end effect on the static deflection signals is discussed,and the importance of the neutral axis shift effect is also illustrated for the asymmetric laminated microcantilever.

关键词： surface stress dna adsorption microcantilever biosensor static deflection clamped-end effect non-uniform deformation

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