检索结果-南通市图书馆

Enhanced efficacy of CTLA-4 fusion anti-caries dna vaccines in gnotobiotic hamsters

Acta Pharmacologica Sinica 2007年第8期28卷 1236-1242页

作者： Feng ZHANG~3 Yu-hong LI~3 Ming-wen FAN~2 Rong JIA Qing-an XU Ji-hua GUO Fei YU Qi-wei TIAN Key Laboratory for Oral Biomedical Engineering of Ministry of Education,School and Hospital of Stomatology,Wuhan University,Wuhan 430079,China Key Laboratory for Oral Biomedical Engineering of Ministry of Education School and Hospital of Stomatology Wuhan University Wuhan China

Aim:To evaluate the comparative immunogenicity and protective efficacy of thecytotoxic T-lymphocyte-associated antigen 4(CTLA-4)fusion anti-caries dnavaccines pGJA-P/VAX1,pGJA-P,and non-fusion anti-caries dna constructpGLUA-P in *** addition,the ability of CTLA-4 to target pGJA-P/VAX 1-encoding antigen to dendritic cells was tested in ***:All dnaconstructs contain genes encoding the A-P regions of a cell surface protein(PAc)and the glucan binding(GLU)domain of glucosyltransferases(GTFs)of cari-ogenic organism Streptococcus *** dendritic cells were mixed withthe CTLA-4-Ig-GLU-A-P protein expressed by pGJA-P/VAX1-transfected cellsand analyzed by flow *** hamsters were immunized with anti-caries dna vaccines by intramuscular injection or intranasal *** responses to a representative antigen PAc were assayed by ELISA,andcaries protection was evaluated by Keyes caries ***:A flow cytometricanalysis demonstrated that CTLA-4-Ig-GLU-A-P protein was capable of bind-ing to human dendritic cells,pGJA-P/VAX1 and pGJA-P induced significantlyhigher specific salivary and serum anti-PAc antibody responses than pGLUA-ESignificantly fewer caries lesions were also observed in hamsters immunized withpGJA-P/VAX1 and *** was no significant difference in the anti-PAcantibody level or caries scores between pGJA-P/VAX1 and ***:Antigen encoded by CTLA-4 fusion anti-caries dna vac-cine pGJA-P/VAX1 could specifically bind to human dendritic cells through theinteraction of CTLA-4 and B7 *** antigen to CTLA-4 has beenproven to greatly enhance the immunogenicity and protective efficacy of anti-caries dna vaccines.

关键词： dna vaccines Streptococcus mutans dendritic cells cytotoxic T-lymphocyteassociated antigen 4

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

dna vaccines Approach:From Concepts to Applications

引用

World Journal of vaccines 2014年第2期4卷 50-71页

作者： Vanessa Bastos Pereira Meritxell Zurita-Turk Tessalia Diniz Luerce Saraiva Camila Prosperi De Castro Bianca Mendes Souza Pamela Mancha Agresti Fernanda Alvarenga Lima Vanessa Nathalie Pfeiffer Marcela Santiago Pacheco Azevedo Clarissa Santos Rocha Daniela Santos Pontes Vasco Azevedo Anderson Miyoshi Laboratorio de Genetica Celular e Molecular Instituto de Ciencias BiologicasUniversidade Federal de Minas Gerais(UFMG)Belo HorizonteBrazil Universidade Estadual da Paraiba(UEPB) Joao PessoaBrazil

dna vaccines are the third generation vaccines based on purified plasmid preparations containing transgenes that encode antigenic/therapeutic proteins or peptides capable of triggering an immune response against a wide range of diseases. This vaccine platform presents several attributes that confer distinct advantages over other vaccine technologies in terms of safety, ease of fabrication and stability. Many aspects, such as antigen expression and especially vector design, are under study because of their great influence on immunogenicity and efficacy of dna vaccines. In this regard, with the attempt of improving the efficiency of dna vaccines, co-expression of stimulatory sequences and diverse vector delivery systems are being optimized. With this in mind, this review aims to giving a conceptual approach of dna vaccines, explaining their mechanisms of action and listing the already licensed veterinary dna vaccines presented in the market.

关键词： dna vaccines Vector Design Delivery Systems Approved dna vaccines Bacterial Delivery

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Efficacy Studies of dna vaccines: Rabies and West Nile dna vaccines

Efficacy Studies of DNA Vaccines: Rabies and West Nile DNA V...

引用

2005全国第二届核酸疫苗研讨会

作者： Stephen Wu Joan Plana Duran Mercedes Mourino Noel Tordo Pierre Perrin Diane Hathaway Neil Jennings Kate Jennen Terry Ng Mike Gill Yu-Wei Chiang Hsien-Jue Chu Fort Dodge Animal Health Div.of Wyeth800 5th St.N.W.Fort DodgeIowa 50501 Fort Dodge Veterinaria S.A.Carretera Camprodon s/n -La Riba17813 Vall de BianyaGeronaSpain Unit Antiviral Strategies Institut Pasteur25-28 Rue du Dr Roux75724 Paris Codex 15France Fort Dodge Animal Health Div.of Wyeth800 5th St. N.W.Fort DodgeIowa 50501

The progress of research in nucleic acid immunization promises new avenues to many infectious disease prophylaxis and therapeutics in human and animals. The commercialization and licensing of dna vaccines are subjected to similar if not more stringent regulatory and market conditions as in many conventional vaccines in terms of administration regimen and dose presentation. The efficacy of a rabies dna vaccine containing a plasmid encoding for the rabies virus glycoprotein gene was evaluated in dogs in a single-dose regimen. The extended duration of immunity of the vaccine was demonstrated by challenge with a wild type virus 17 months post vaccination. Similarly, conventional equine West Nile Virus vaccines are available in the United States as a monovalent as well as multivalent combination vaccines containing a number of other viral antigens.

关键词： dna vaccines rabies, west nile

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Epitope dna vaccines against tuberculosis： spacers and ubiquitin modulates cellular immune responses elicited by epitope dna vaccine

引用

第二军医大学学报 2005年第2期26卷 191-191页

作者： Wang QM Sun SH Hu ZL Zhou FJ Yin M Xiao CJ Zhang JC Department of Medical Genetics Second Military Medical University Shanghai 200433 China

Cell-mediated immune responses are crucial in the protection against tuberculosis. In this study, we constructed epitope dna vaccines (p3-M-38) encoding cytotoxic T lymphocyte (CTL) epitopes of MPT64 and 38 kDa proteins of Mycobacterium tuberculosis. In order to observe the influence of spacer sequence (Ala-Ala-Tyr) or ubiquitin (UbGR) on the efficacy of the two CTL epitopes, we also constructed dna vaccines, p3-M-S(spacer)-38, p3-Ub (UbGR)-M-S-38 and p3-Ub-M-38. The immune responses elicited by the four dna vaccines were tested in C57BL/6 (H-2b) mice. The cytotoxicity of T cells was detected by LDH-release method and by enzyme-linked immunospot assay for epitope-specific cells secreting interferon-gamma. The results showed that dna immunization with p3-M-38 vaccine could induce epitope-specific CD8+ CTL response and that the spacer sequence (AAY) only enhanced M epitope presentation. The protein-targeting sequence (UbGR) enhanced the immunogenicity of the two epitopes. The finding that defined spacer sequences at C-terminus and protein-targeting degradation modulated the immune response of epitope string dna vaccines will be of importance for the further development of multi-epitope dna vaccines against tuberculosis.

关键词： dna vaccines immune responses Mycobacterium tuberculosis cytotoxic T lymphocyte protection against dna immunization T cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Granulocyte-macrophage colony-stimulating factor dna prime-protein boost strategy to enhance efficacy of a recombinant pertussis dna vac-cine

引用

Acta Pharmacologica Sinica 2006年第11期27卷 1487-1494页

作者： Qing-tian LI~(2,3,5)Yong-zhang ZHU~(2,3,5)Jia-you CHU~(2,6)Ke DONG~3 Ping HE~3 Chun-yan FENG~3 Bao-yu HU~3 Shu-min ZHANG~4 Xiao-kui GUO~(3,6)2 Institute of Medical Biology,Peking Union Medical College and Chinese Academy of Medical Sciences,Kunming 650118,China 3 Department of Medical Microbiology and Parasitology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China 4 Chinese National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China Institute of Medical Biology Peking Union Medical College and Chinese Academy of Medical Sciences Kunming China Department of Medical Microbiology and Parasitology Shanghai Jiao Tong University School of Medicine Shanghai China Chinese National Institute for the Control of Pharmaceutical and Biological Products Beijing China

Aim:To investigate a new strategy to enhance the efficacy of a recombinantpertussis dna *** strategy is co-injection with cytokine plasmids asprime,and boosted with purified homologous ***:A recombinantpertussis dna vaccine containing the pertussis toxin subunit 1(PTS 1),fragmentsof the filamentous hemagglutinin(FHA)gene and pertactin(PRN)gene encodingfilamentous hemagglutinin and pertactin were ***/c mice were im-munized with several dna vaccines and antigen-specific antibodies anti-PTS1,anti-PRN,anti-FHA,cytokines interleukin(IL)-10,IL-4,IFN-γ,TNF-α,and spleno-cyte-proliferation assay were used to describe immune ***:Therecombinant dna vaccine could elicit similar immune responses in mice as that ofseparate plasmids encoding the 3 fragments,*** immunized withdna and boosted with the corresponding protein elicited more antibodies thanthose that received dna as *** particular,when the mice were co-immu-nized with murine granulocyte-macrophage colony-stimulating factor plasmidsand boosted with proteins,all 4 cytokines and the 3 antigen-specific antibodieswere significantly increased compared to the pVAX1 ***-PTS1,anti-FHA,IL-4 and TNF-α elicited in the colony stimulating factor(CSF)prime-proteinboost group showed significant increase compared to all the other ***:This prime and boost strategy has proven to be very useful in im-proving the immunogenicity of dna vaccines against pertussis.

关键词： granulocyte-macrophage colony-stimulating factor pertussis dna vaccines

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Enhancing cellular immune response to HBV M dna vaccine in mice by codelivery of interleukin-18 recombinant

引用

Journal of Zhejiang University Science 2004年第4期5卷 467-471页

作者：陈建忠朱海红刘克洲陈智 InstituteofImmunology SchoolofMedicineZhejiangUniversityHangzhou310031China InstituteofInfectiousDiseases FirstAffiliatedHospitalSchoolofMedicineZhejiangUniversityHangzhou310003China

Objective:To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV dna ***:BALB/c mice were immunized with pCMV-M alone or co-immunized with pcdna3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA;splenocytes were isolated for detecting specific CTL response and cytokine assay in ***:The anti-HBs antibody level of mice co-immunized with pcdna3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone,but there was not significantly different (P>0.05).Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcdna3-18 and pCMV-M was significantly enhanced (P0.05).Conclusion:The plasmid encoding IL-18 together with HBV M gene dna vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.

关键词： Interleukin-18 Hepatitis B virus dna vaccines Immune response

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nucleic acid vaccines: A taboo broken and prospect for a hepatitis B virus cure

引用

World Journal of Gastroenterology 2021年第41期27卷 7005-7013页

作者： Efthymios P Tsounis Athanasia Mouzaki Christos Triantos Division of Gastroenterology Department of Internal MedicineUniversity of PatrasPatras 26504Greece Division of Hematology Department of Internal MedicineUniversity of PatrasPatras 26504Greece

Although a prophylactic vaccine is available,hepatitis B virus(HBV)remains a major cause of liver-related morbidity and *** treatment options are improving clinical outcomes in chronic hepatitis B;however,true functional cure is currently the exception rather than the *** acid vaccines are among the emerging immunotherapies that aim to restore weakened immune function in chronically infected *** vaccines in particular have shown promising results in vivo by reducing viral replication,breaking immune tolerance in a sustained manner,or even decimating the intranuclear covalently closed circular dna reservoir,the hallmark of HBV *** dna vaccines encoding surface antigens administered by conventional injection elicit HBVspecific T cell responses in humans,initial clinical trials failed to demonstrate additional therapeutic benefit when administered with nucleos(t)ide *** an attempt to improve vaccine immunogenicity,several techniques have been used,including codon/promoter optimization,coadministration of cytokine adjuvants,plasmids engineered to express multiple HBV epitopes,or combinations with other *** vaccine delivery by electroporation is among the most efficient strategies to enhance the production of plasmid-derived antigens to stimulate a potent cellular and humoral anti-HBV *** results suggest that dna vaccination via electroporation efficiently invigorates both arms of adaptive immunity and suppresses serum HBV *** contrast,the study of mRNA-based vaccines is limited to a few in vitro experiments in this *** studies are needed to clarify the prospects of nucleic acid vaccines for HBV cure.

关键词： Chronic hepatitis B Therapeutic vaccination Nucleic acid vaccines dna vaccines Electroporation Immunotherapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Enhancing dna vaccine potency against hantavirus by co-administration of interleukin-12 expression vector as a genetic adjuvant

引用

Chinese Medical Journal 2005年第4期118卷 313-319页

作者： ZHENGLan-yan MOULing LINSong LURun-ming LUOEn-jie DepartmentofMicrobiologyandParasitology ChinaMedicalUniversityShenyang110001China DepartmentofLaboratoryMedicine MunicipalHospitalofInfectiousDiseaseShenyang110003China

Background The heavy incidence and mortality of hemorrhagic fever with renal syndrome, as well as no specific drugs in curing the disease, clearly indicate the need for development Of the more effective hantavirus vaccine. Refining the dna vaccination strategy to elicit more clinically efficacious immune responses is now under intensive investigation. In the present study, we examined the effects of using an interleukin-12 expression plasmid as a genetic adjuvant to enhance the immune responses induced by a dna vaccine based on the S gene encoding nucleocapsid protein against hantavirus. Methods BALB/c mice were immunized three times by intramuscular inoculations of dna vaccine encoding of hantanvirus nucleoeapsid protein alone or in combination with a plasmid expressing murine interleukin-12 (pcIL-12). Booster immunizations were employed 2 times at 2-week interval. To evaluate the Immoral and cellular immune responses, antigen-specific lymphocyte proliferation and antibody production were assayed by MTT method and ELISA respectively. The level of interleukin-4 and interferon-gamma in the splenic lymphocytic cultured supernatant were detected with ELISA kit at day 5, 10, 17, 35 and 42 after primary immunization. Results Antigen-specific IgG antibodies was increased markedly at day 17 in the experiment groups and reached a plateau after day 35. As pcIL-12 co-injected, a significant inhibition of antigen-specific IgG levels was displayed over the period and the antibody mean titre was decreased to only about 1: 50 at day 42 after primary immunization, significantly lower than the group immunized with pcdna3.1 + S alone, in which the mean titre was about 1:70. Interferon-gamma was increased remarkably by the co-injection of pcIL-12 compared with the injection of pcdna3.1 + S alone. However, the production of interleukin-4 was inhibited by pcIL-12 coinjection. Furthermore, pcIL-12 co-injection efficiently enhanced antigen-specific lymphocyte proliferation. Conclusion Hu

关键词： dna vaccines hantavirus gene adjuvant interleukin-12 nucleocapsid proteins

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enhancement of dna vaccine-induced immune responses by a 72-bp element from SV40 enhancer

引用

Chinese Medical Journal 2007年第6期120卷 496-502页

作者： LI Hai-shan LIU Yong LI Ding-feng ZHANG Ran-ran TANG Hai-li ZHANG Yu-wei HUANG Wei LIU Ying PENG Hong XU Jian-qing HONG Kun-xue SHAO Yi-ming State Key Laboratory for Infectious Disease Prevention and Control National Center for AIDS/STD Control and Prevention Chinese Center for Disease 100050 China

Background Although dna vaccine is considered as the next generation of vaccine, most dna vaccine candidates are still suffering from the relatively weak immunogenicity despite the increased dosage of plasmid dna administered. In order to enhance the immune responses elicited by a codon-optimized HIV gag dna vaccine, a modified plasmid vector pDRVI1.0 and a booster immunization with replicating Tiantan vaccinia （RTV） strain expressing the same gene were employed. Methods Vector pDRVI1.0 was constructed through inserting the 72-bp element from the SV40 enhancer, which was reported promoting nuclear transport of plasmid dna, to the upstream of cytomegalovirus enhancer/promoter region of the plasmid vector pVR1012. Gene expression levels from expression plasmids based on pDRVI1.0 and pVR1012 were tested. Humoral and cellular immune responses induced by dna vaccine alone or dna prime-RTV boost regimen were determined in mice. Results It was shown that the 72-bp element significantly enhanced the gene expression level in non-dividing cells. gag-specific humoral and cellular immune responses induced by dna vaccination were both significantly improved, while the Thl/Th2 balance was not obviously affected by the 72-bp element. RTV boosting further significantly enhanced dna vaccine-palmed antibody and T cell responses in a Thl-biased manner. Conclusions The 72-bp SV40 enhancer element should be included in the dna vaccine vector and RTV strain is a very efficient live vector for boosting immunization.

关键词： dna vaccines SV40 enhancer prime-boost vaccinia Tiantan strain

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enhanced immunization after intranasal coadministration of Escherichia coli heat-labile enterotoxin B subunit and human papillomavirus 16-L1 dna vaccine

引用

Chinese Medical Journal 2006年第5期119卷 408-411页

作者： WANG Jing ZHAO Chang-an WANG Kai ZHENG Jin WANG Yi-li SI Lü-sheng Key Laboratory of Biomedical Information Engineering of Education Ministry Institute for Cancer Research School of Life Science ＆ Technology Xi＇an Jiaotong University Xi＇an 710061 China

Human papillomavirus （HPV）, mainly types 16 and 18, are the most important initiating agents of cervical cancer. Prevention of high-risk HPV infections is a potentially effective approach to control HPV associated c... 详细信息

关键词： Escherichia coil heat-labile enterotoxin B subunit human papillomavirus type 16 dna vaccines

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：