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Synthetic and Systems Biotechnology 2024年第2期9卷 242-249页

作者： Qiqun Peng Weiwei Bao Binan Geng Shihui Yang State Key Laboratory of Biocatalysis and Enzyme Engineering and School of Life SciencesHubei UniversityWuhan430062China

lactate is an important monomer for the synthesis of poly-lactate(PLA),which is a substitute for the petrochemical *** achieve the goal of high lactate titer,rate,and yield for commercial production,efficient lactate production pathway is needed as well as genetic targets that affect high lactate production and *** this study,an LldR-based d-lactate biosensor with a broad dynamic range was first applied into Zymomonas mobilis to select mutant strains with strong GFP fluorescence,which could be the mutant strains with increased d-lactate ***,LldR-based d-lactate biosensor was combined with a genome-wide CRISPR interference(CRISPRi)library targeting the entire genome to generate thousands of mutants with gRNA targeting different genetic targets across the whole ***,two mutant libraries were selected containing 105 and 104 mutants with different interference sites from two rounds of fluorescence-activated cell sorting(FACS),*** genetic targets of ZMO1323 and ZMO1530 were characterized and confirmed to be associated with the increased d-lactate production,further knockout of ZMO1323 and ZMO1530 resulted in a 15%and 21%increase of d-lactate production,*** work thus not only established a high-throughput approach that combines genome-scale CRISPRi and biosensor-assisted screening to identify genetic targets associated with d-lactate production in ***,but also provided a feasible high-throughput screening approach for rapid identification of genetic targets associated with strain performance for other industrial microorganisms.

关键词： d-lactate Biosensor LldR Genome-wide CRISPRi FACS Zymomonas mobilis

Soluble intercellular adhesion molecule-1,d-lactate and diamine oxidase in patients with inflammatory bowel disease

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World Journal of Gastroenterology 2009年第31期15卷 3916-3919页

作者： Wei-Bing Song Yong-Hui Lv Zhen-Shu Zhang Ya-Nan Li Li-Ping Xiao Xin-Pei Yu Yuan-Yuan Wang Hong-Li Ji Li Ma department of Gerontology Guangzhou General Hospital of the PLA Guangzhou 510010 Guangdong Province China The Traditional Chinese'Medical Hospital of Guangzhou City Guangzhou 510010 Guangdong Province China Institute for digestive diseases Nanfang Hospital Southern Medical University Guangzhou 510515 Guangdong Province China Suzhou River Cadre's Sanatorium Second Artillery Rest of the Cadres of the PLA Beijing 100086 China

AIM： To study the levels of serum soluble intercellular adhesion molecule-1 （sICAM-1）, plasma d-lactate and diamine oxidase （dAO） in patients with inflammatory bowel disease （IBd）, and the potential clinical significance. METHOdS： Sixty-nine patients with IBd and 30 healthy controls were included in this study. The concentration of sICAM-1 was detected with enzyme-linked immunosorbent assay, the level of d-lactate and dAO was measured by spectroscopic analysis, and the number of white blood cells （WBC） was determined by routine procedure. RESULTS： The levels of sICAM-I, dAO, and WBC in IBd patients were significantly higher than those in the control group （P 〈 0,01）, sICAM-I in IBd patients was found to be closely related to the levels of dAO and d-lactate （212.94 ± 69.89 vs 6.35 ± 2.35, P = 0.000）, dAO 212.94 ± 69.89 vs 8.65 ± 3.54, P = 0.000） and WBC （212.94 ± 69.89 vs 7.40 ± 2.61, P = 0.000）, but no significant difference was observed between patients with ulcerative colitis and patients with Crohn＇s disease. The post-treatment levels of sICAM-I, d-lactate and WBC were significantly lower than before treatment （sICAM-I 206.57 ± 79.21 vs 146.21 ± 64.43, P = 0.000）, （d-lactate 1.46 ± 0.94 vs 0.52± 0.32, P = 0.000） and （WBC 7.24 ± 0.2.33 vs 5.21 ± 3.21, P = 0.000）. CONCLUSION： sICAM-1, d-lactate and dAO are closely related to the specific conditions of IBd, and thus could be used as a major diagnostic index.

关键词： Inflammatory bowel diseases Intercellular adhesion molecule-1 d-lactate diamine oxidase

超早期EN联合乌司他丁治疗SAP对免疫功能、d-lactate、endotoxin的影响

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中文科技期刊数据库（全文版）医药卫生 2023年第11期 44-47页

作者：李军郴州市第一人民医院湖南郴州423001

探讨分析急性重症胰腺炎(severe acute pancreatitis,SAP)患者采用超早期肠内营养(enteral nutrition, EN)联合乌司他丁治疗对免疫功能、d-lactate、endotoxin产生的影响效应。方法本次研究时间节点为2022年1月—2022年12月,选择了在... 详细信息

探讨分析急性重症胰腺炎(severe acute pancreatitis,SAP)患者采用超早期肠内营养(enteral nutrition, EN)联合乌司他丁治疗对免疫功能、d-lactate、endotoxin产生的影响效应。方法本次研究时间节点为2022年1月—2022年12月,选择了在我院实施治疗的SAP患者80例,基于随机数字双盲法分组,对照组的治疗仅行超早期EN,观察组行超早期EN+乌司他丁治疗,对两组患者最后治疗结局进行对比和分析。结果与对照组相比,观察组肠鸣音恢复所需时间、体温恢复正常时间、腹痛消失时间均更短(P0.05),经过治疗,患者症状第 5 天发生了明显变化(P0.05),经过治疗,观察组变化优于对照组(P0.05),经过治疗,观察组变化优于对照组(Pd3+、Cd4+、Cd8+水平差异不明显(P>0.05);经过治疗,观察组变化优于对照组(Pd-lactate、endotoxin指标起到改善作用,临床价值显著。

关键词：超早期肠内营养急性重症胰腺炎免疫功能 d-lactate endotoxin

Controversies about the use of serological markers indiagnosis of in flammatory bowel disease

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World Journal of Gastroenterology 2010年第2期16卷 279-280页

作者： Xie, Qin Gan, Hua-Tian Sichuan Univ Dept Geriatr W China Hosp Chengdu 610041 Sichuan Peoples R China Sichuan Univ Dept Gastroenterol W China Hosp Chengdu 610041 Sichuan Peoples R China

The serological markers are increasingly used in diagnosis of inflammatory bowel disease(IBd).d-lactate and diamine oxidase are new indicators that can be used to reveal the damage to intestinal mucosa and permeability alteration in *** the two biological markers seem more sensitive,recent clinical trials and animal experiments have shown controversies about the use of them in diagnosis of ***, these markers should be interpreted cautiously and further prospective studies are needed to establish their clinical role in diagnosis of IBd.

关键词： Inflammatory bowel diseases d-lactate diamine oxidase Intestinal permeability diagnosis

Effect of Lianshu preparation on lipopolysaccharide-induced diarrhea in rats

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World Journal of Gastroenterology 2009年第16期15卷 2009-2015页

作者： Jun Liu Rong Wan Xuan-Fu Xu Xing-Peng Wang Wen-Juan Yang Yu-Jing Xia Hua Liu Qian-Lin Yan de-Xin Yan Chuan-Yong Guo department of Gastroenterology Tenth People's Hospital of Tongji University Shanghai 200072 China department of Chinese Medicine Tenth People's Hospital of Tongji University Shanghai 200072 China Institute of Chinese Medicine of Tongji University Shanghai 200092 China

AIM： To investigate the effect of Lianshu preparation on lipopolysaccharide （LPS）-induced diarrhea in rats. METHOdS： A diarrhea model was established in Sprague dawley rats via injection of 1 mL of 30 mg/kg LPS. A total of 40 rats were randomly divided into normal group, LPS group, LPS ＋ Lianshu group, LPS ＋ berberine group （n = 10 in each group）. Their intestinal mucosal barrier and frequency of diarrhea were observed. Levels of glucose, serum Na^＋, K^＋, Cl and hematocrit, plasma nitrogen monoxide （NO）, diamine oxidase （dAO）, and d （-）-lactate were measured. The number of IgA＋ plasma cells in small intestine was detected and SIgA levels in the intestinal fluid were measured. The antipyretic activity of Lianshu preparation in rats was evaluated using Brewer＇s yeast-induced pyrexia （10 mL/kg of 20% aqueous suspension）. Acetaminophen （250 mg/kg, intragastric administration, bid） was comparison. Temperature used as a standard drug for was recorded 1 h before and 6 h after Brewer＇s yeast injection. Finally, small intestina transmission in mice treated with Lianshu was detected after intraperitoneal injection of methyl prostigmin （2 mg/kg）. Atropine （10 g/kg） was used as a control. The ink content in intestine was determined and the total length of intestine was measured. RESULTS： The frequency of diarrhea was higher in LPS group than in LPS ＋ Lianshu group and LPS ＋ berberine group （36.70± 5.23 vs 28.50 ±4.06 and 32.70±9.30 respectively, P 〈 0.01）, and lower in LP5 ＋ Lianshu group than in LPS ＋ berberine group （P = 0.03）. The levels of Na＋, glucose, Cl, K^＋ were significantly lower in LPS ＋ Lianshu group than in LPS ＋ berberine group （140.35±3.19 mmol/L vs 131.99±4.86 mmol/L, 8.49 ±1.84 mmol/L vs 6.54±2.30 mmol/L, 106.29± 4.41 mmol/L vs 102.5±1.39 mmol/L, 5.08±0.66 mmol/L vs 4.32 ± 0.62 mmol/L respectively, P 〈 0.05）. The level of hematocrit was lower in LPS ＋ Lianshu group than in LPS ＋ berberine group （0.50% ±0.07% vs 0.59%± 0.10% respectively, P 〈 0.05）. The plasma

关键词： Lianshu preparation Lipopolysaccharide diarrhea Nitrogen monoxide d-lactate

Effects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats

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World Journal of Gastroenterology 2008年第38期14卷 5868-5875页

作者： Ying-Jie Sun Wei-Min Chen Tie-Zheng Zhang Hui-Juan Cao Jin Zhou department of Anesthesiology ShengJing Hospital China Medical University Shenyang 110004 Liaoning Province China department of Anesthesiology the Northern Hospital of China Shenyang 110016 Liaoning Province China

AIM： To investigate the tight junction protein expressions of intestinal mucosa in an experimental model of cardiopulmonary bypass （CPB） in rats. METHOdS： Thirty anesthetized rats were randomly divided into two groups： Group S （n = 10） served as sham operation and group C （n = 20） served as CPB which underwent CPB for 1 h. Expression of occludin and zonula occludens-1 （ZO-1） were determined by Western blotting and immunotochemistry, respectively. Plasma levels of diamine oxidase （dAO） and d-lactate were determined using an enzymatic spectrophotometry. RESULTS： Immunohistochemical localization of occludin and ZO-1 showed disruption of the tight junctions in enterocytes lining villi at the end of CPB and 2 h after CPB. The intensities of the occludin and ZO-i at the end of CPB were lower than those of control group （76.4% ± 22.5% vs 96.5% ± 28.5% and 62.4% ± 10.1% vs 85.5% ±25.6%, P 〈 0.05） and were further lower at 2 h after CPB （50.5% ± 10.5% and 45.3% ± 9.5%, P 〈 0.05）. Plasma d-lactate and dAO levels increased significantly （8.688 ± 0.704 vs 5.745 ± 0.364 and 0.898 ± 0.062 vs 0.562 ± 0.035, P 〈 0.05） at the end of CPB compared with control group and were significantly higher at 2 h after CPB than those at the end of CPB （9.377 ± 0.769 and 1.038 ± 0.252, P 〈 0.05）. There were significant negative correlations between occludin or ZO-1 expression and dAO （r^2 = 0.5629,r^2 = 0.5424, P 〈 0.05） or d-lactate levels （r^2 = 0.6512,r^2 = 0.7073, P 〈 0.05） both at the end of CPB and 2 h after CPB. CONCLUSION： CPB markedly down-regulates the expression of occludin and ZO-1 proteins in intestinal mucosa of rats. The close correlation between expression of tight junctions （TJs） and plasma levels of dAO or d-lactate supports the hypothesis that intestinal permeability increases during and after CPB because of decreases in the expressions of TJs.

关键词： Cardiopulmonary bypass Occludin ZO-1 Tight junction diamine oxidase d-lactate

Increased intestinal permeability in pathogenesis and progress of nonalcoholic steatohepatitis in rats

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World Journal of Gastroenterology 2007年第11期13卷 1732-1736页

作者： Xi Jin Chao-Hui Yu Guo-Cai Lv You-Ming Li digestive department of the First Affiliated Hospital Medical School Zhejiang University Hangzhou 310003 Zhejiang Province China Clinical Laboratory of the First Affiliated Hospital Medical School Zhejiang University Hangzhou 310003 Zhejiang Province. China

AIM： To investigate whether increased intestinal permeability contributes to the pathogenesis and progress of nonalcoholic steatohepatitis by observing its dynamic change in rat models. METHOdS： Rat models of nonalcoholic steatohepatitis were established by giving a fat-rich diet. The rats were sacrificed at wk 8, 12 and 16 during the study. Rats fed with normal diet were taken as control. Plasma d-lactate, plasma diarnine oxidase, serum lipids and liver transarninases were measured in blood of the femoral artery. Hepatic steatosis and inflammation were assessed by haematoxylin-eosin staining. RESULTS： A rat model of nonalcoholic steatohepatitis was established successfully. Plasma d-lactate level in model group at wk 8, 12 and 16 and diarnine oxidase level in model group at wk 12, 16 increased significantly compared with those in control group. There were notable differences of d-lactate and diarnine oxidase level in model group between wk 8 and 12 as well as between wk 12 and 16. Serum lipids, liver transaminases and liver injury also increased with disease development CONCLUSION： Increased intestinal permeability caused by intestinal bacterial overgrowth and endotoxin-induced intestinal destruction exists in rats with nonalcoholic steatohepatitis, which may partially explain the pathogenesis and progress of this disease.

关键词： Nonalcoholic steatohepatitis Intestinal permeability d-lactate diamine oxidase

Functional changes of intestinal mucosal barrier insurgically critical patients

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World Journal of Emergency Medicine 2010年第3期1卷 205-208页

作者： Yuan-yuanGuo Mu-linLiu Xian-di He Cong-qiao Jiang Rui-lin Liu department of Gastrointestinal Surgery Afliated Hospital of Bengbu MedicalCollegeBengbu233004China

BACKGROUNd： The gut is capable of inducing multiple organ dysfunction syndrome （MOdS）. In the diagnosis and treatment of critical ill patients, doctors should pay particular attention to the protection or recovery of intestinal barrier function. However, no reliable diagnostic criteria are available clinically. This study aimed to assess the changes of intestinal mucosal barrier function in surgically critical ill patients as well as their signi？ ***： Thirty-eight surgically critical ill patients were enrolled as a study group （APACHE II〉8 scores）, and 15 non-critical ill patients without intestinal dysfunction were selected as a control group （APACHE II〈6）. General information, symptoms, physical signs, and APACHE II scores of the patients were recorded. The patients in the study group were subdivided into an intestinal dysfunction group （n=26） and a non-intestinal dysfunction group （n=12）. Three ml venous blood was collected from the control group on admission and the same volume of plasma was collected from the study group both on admission and in the period of recovery. The plasma concentrations of endotoxin, diamine oxidase （dAO）, d-lactate, and intestinal fatty-acid binding protein （iFABP） were detected respectively. The data collected were analyzed by the SPSS 17.0 software for Windows. RESULTS： The levels of variables were significantly higher in the study group than in the control group （P〈0.01）. They were higher in the intestinal dysfunction group than in the non-intestinal dysfunction group （dAO P〈0.05, endotoxin, d-lactate, iFABP P〈0.01）. In the non-intestinal dysfunction group compared with the control group, the level of endotoxin was not significant （P〉0.05）, but the levels of dAO, d-lactate and iFABP were statistically significant （P〈0.05）. The levels of variables in acute stage were higher than those in recovery stage （P〈0.01）.The death group showed higher levels of variables than the survival group （endotoxin and d-lactate P〈0.01, dAO a

关键词： Intestinal mucosal barrier Endotoxin diamine oxidase d-lactate Intestinal fatty-acid binding protein