检索结果-南通市图书馆

Overall Estimation of Anti-Oxidant Activity by Mammal Macrophage

Open Journal of Rheumatology and Autoimmune Diseases 2014年第1期4卷 13-21页

作者： Nobuo Yamaguchi Natsuko Kawada Xinfeng Jia Kazuhiro Okamoto Katsuko Okuzumi Rui chen Takashi Takahashi Department of Fundamental Research for cAM Kanazawa Medical University Ishikawa Japan Ishikawa Natural Medicinal Products Research center Kanazawa Japan Kanazawa University Graduate School of Medical Science Kanazawa Japan Laboratory of Infectious Diseases Graduate School of Infection Control Sciences Kitasato University Tokyo Japan. Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Super oxide anion had been known as a critical role in a phagocytic cell when microorganisms invade inside. However, this radical seldom need for a clean environment such in a developed country. Moreover, this agent sometimes triggered as serious factor in the site of inflammation. There are many papers which concerned newly found anti-oxidative substances. However, many reports only focused on the molecular basis in vitro, how oxidative stress damaged the human tissue. The key factor for oxidative stress needs to be discussed first for immunological standpoint, such as armed reduction treaty. In other words, minimum armed force is necessary for an invasion by outer or inner irregular cells. On the basis of phagocytic function of the cell in vivo, we tried to access anti-oxidative activity by employing peritoneal murine macrophage and human monocyte. So, the purpose of this study is to establish a conventional but tailor made system for which is useful for evaluating the anti-oxidative activity to different constition. Especially in human peripheral monocyte, the sample was tested each constitution such in granulocyte rich type and lymphocyte rich typ. In order to establish the direct estimation by phagocytic cell, a murine peritoneal exudates cell suspension was obtained after one week of oral administration with anti-oxidative sample. The samples we selected were traditional chinese medicine, Shi-Quan-Da-Bu-Tang (SDT), Bu-Zong-Ye-Qi-Tang (BYT) and Shao-chin-Rong-Tang scRT), Agaricus, chlorella, and Propolis. The amount of generated super oxide anion was calculated through measuring the optical density change of this supernatant using cytochrome-c which was reduced by generated super oxide anion in the phagocytic cell. The generated super oxide anion after administration of Agaricus, chlorella, SDT, BYT, scRT and Propolis were 2.64, 1.95, 1.24, 1.25, 2.88, 2.55 × 10﹣5 mmol/ml, respectively, whereas 2.85 × 10﹣5 mmol/ml in control group. The fermented sample from SDT

关键词： Anti-Oxidant Sample Super Oxide Anion Neutrophil Macrophage cytochrome-c constitution

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Metallated Schiff-Base Macromolecules as Alternative Metalloprotein Electron Transfer Intermediates

引用

Journal of Surface Engineered Materials and Advanced Technology 2020年第2期10卷 34-54页

作者： Al c. Farao Rachel Fanelwa Ayaji Meryck Ward Priscilla GL Baker SensorLab Research Group Department of Chemistry University of the Western Cape PB X17 Bellville South Africa

In the construction of biosensors, enzymes function as mediators converting biological signals generated by specific biological processes, into electrochemical signals. The ideology of bio-sensor design is retention of electron transfer activity of the enzyme utilizing superior interfacial architecture. In this work a Schiff-base macromolecule has been synthesized by reflux of 2, 3-diaminonaphthalene and pyrrole-2-carboxaldehyde starting materials. The Schiff-base ligand was subsequently complexed with Fecl2?4H2O under reflux, to produce the Fe-Schiff-base complex. The Schiff-base ligand and Fe-Schiff-base complex were characterized using nuclear magnetic resonance (NMR) spectroscopy, Ultra Violet/Visible (UV/Vis) spectroscopy, Fourier transfer infrared resonance (FTIR) and electron energy loss spectroscopy (EELS) to confirm the structure of the synthesis products. NMR spectroscopy confirmed the imide linkage of Schiff-base formation as two symmetrical peaks at 8.1 and 7.7 ppm respectively. comparison of starting materials and product spectra by UV/Vis spectroscopy confirmed the disappearance of the diaminonaphthalene peak at 250 nm as evidence of complete conversion to product. FTIR spectroscopy of the Schiff-base ligand confirmed the formation of the imine bond at 1595 cm-1. EELS spectra comparing Fecl2?4H2O and the Fe-Schiff-base complex, showed good agreement in the energy loss profiles associated with changes to the electronic arrangement of Fe d-orbitals. EDS clearly identified a spectral band for Fe (7 - 8 eV) in the Fe-Schiff-base complex. Electrochemical evaluation of the Fe-Schiff-base complex was compared to the electrochemical signature of denatured cytochrome-c using cyclic voltammetry and square wave voltammetry. The Fe2+/Fe3+ quasi-reversible behavior for iron in the metallated complex was observed at -0.430 V vs. Ag/Agcl, which is consistent with reference values for iron in macromolecular structures.

关键词： cytochrome-c Macromolecule Metallated Metalloproteins Schiff-Base

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

锰锌联合作用下大鼠生精细胞caspase-3和cyto-c的表达变化

引用

河南职工医学院学报 2012年第5期24卷 577-579,I0001页

作者：郭海才秀莲王国秀河南卫生职工学院正常人体结构教研室郑州451191 遵义医学院组织学与胚胎学教研室贵州遵义563003

目的研究锰和锌对生精细胞caspase-3、cyto-c表达的影响。方法健康雄性SD大鼠80只随机分为10组,空白对照组,Zncl2对照组,15 ***-1和30 ***-1Mncl2组;15 ***-1Mncl2+Zncl2组和30 ***-1Mncl2+Zncl2组。锰组分别给予Mncl2.4H2O腹腔注射4周和6周;Zncl2对照组给予100 mg Zn2+.kg-1饲料口服;锰加锌组分别染锰4周后再给予含锌饲料口服。各组大鼠分别于第4周末和第6周末处死,免疫组化法检测睾丸caspase-3、cyto-c表达。结果与空白组比较,各锰组和锰加锌组caspase-3阳性细胞率均升高,cyto-c阳性细胞率均降低(Pcaspase-3和cyto-c阳性细胞率分别较4周升高和降低(Pcaspase-3和cyto-c阳性细胞率分别较15 ***-1组升高和降低(Pcaspase-3和cyto-c阳性细胞率分别降低和升高。各组caspase-3和cyto-c阳性细胞率呈负相关(r=-0.817,Pcaspase-3和cyto-c表达,并随染锰时间和剂量的增加而分别增加和降低,存在一定的时间-效应和剂量-效应关系,摄入含锌100 ***-1的食物可抑制锰所诱发的caspase-3表达。

关键词：锰锌生精细胞 caspase-3 cytochrome-c

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：