检索结果-南通市图书馆

Troglitazone Induced Apoptosis via PPARγ Activated POX-induced ROS Formation in HT29 cells

Biomedical and Environmental Sciences 2011年第4期24卷 391-399页

作者： WANG Jing LV XiaoWen SHI JiePing HU XiaoSong DU YuGuo State Key Laboratory of Environmental chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Feed Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China 3.College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijing 100083China college of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China

Objective In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells,the effects of PPARγ and POX-induced ROS were *** [3-（4,5）-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide （MTT） assay,Annexin V and PI staining using FAcS,plasmid transfection,ROS formation detected by DcFH staining,RNA interference,RT-PcR RT-QPcR,and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPARγ pathway and POX-induced ROS formation in HT29 *** Troglitazone was found to inhibit the growth of HT29 cells by induction of *** this process,mitochondria related pathways including ROS formation,POX expression and cytochrome c release increased,which were inhibited by pretreatment with GW9662,a specific antagonist of PPARγ.These results illustrated that POX upregulation and ROS formation in apoptosis induced by troglitazone was modulated in PPARγ-dependent ***,the inhibition of ROS and apoptosis after POX siRNA used in troglitazone-treated HT29 cells indicated that POX be essential in the ROS formation and PPARγ-dependent apoptosis induced by *** The findings from this study showed that troglitazone-induced apoptosis was mediated by POX-induced ROS formation,at least partly,via PPARγ activation.

关键词： Troglitazone Apoptosis HT29 POX ROS formation PPARγ cytochrome c release

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

cdk2 acts upstream of mitochondrial permeability transition during paclitaxel-induced apoptosis

引用

Protein & cell 2011年第7期2卷 543-553页

作者： Xiao-Xi Guo Hanna Kim Yang Li Hyungshin Yim Seung Ki Lee Ying-Hua Jin Key Laboratory for Molecular Enzymology&Engineering of the Ministry of Education Jilin UniversityChangchun 130012China Division of Pharmaceutical Biosciences Research Institute for Pharmaceutical SciencesCollege of PharmacySeoul National UniversitySeoul 151-742Korea

Sequential activation of cyclin-dependent kinases(cdks)controls mammalian cell *** we demonstrate that the upregulation of cyclin-dependent kinase 2(cdk2)activity coincides with the loss of mitochondrial membrane potential(MMP)in paclitaxel-induced *** expression of the dominant negative cdk2(cdk2-dn)and a specific cdk2 inhibitor,p21WAF1/cIP1,effectively suppresses the loss of MMP,the release of cytochrome c,and subsequent activation of caspase-3 in paclitaxel-treated *** forced activation of cdk2 by overexpression of cyclin A dramatically promotes these *** further show that cdk2 activation status does not interfere with a procedure that lies downstream of cytochrome c release induced by Bax *** findings suggest that cdk2 kinase can regulate apoptosis at earlier stages than mitochondrial permeability transition and cytochrome c release.

关键词： apoptosis cyclin-dependent kinase 2 cytochrome c release mitochondrial membrane potential paclitaxel

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Study on the structure and composition of aortic valve calcific deposits:Etiological aspects

引用

Journal of Biophysical chemistry 2011年第1期2卷 19-25页

作者： Rafael.M.Prieto I.Gomila O.Sohnel A.costa-Bauza O.Bonnin Felix Grases Laboratory of Renal Lithiasis Research University Institute of Health Sciences Research(IUNICS)University of Balearic IslandsPalma of MallorcaSpain cIBER Fisiopatología Obesidad y Nutricion(cB06/03) Instituto de Salud Carlos IIIMadridSpain University of J.E.Purkyne Faculty of Environmental StudiesUsti n.L.Czech Republic University Hospital Son Dureta Palma of MallorcaSpain

The structures and chemical compositions of valve calcific deposits were investigated. The deposits was chosen arbitrarily and subjected to chemical analysis, observation with scanning microscope, semi-quantitative determination of ca, Mg, Na, K, P and c elements by energy dispersive X-ray, X-ray diffraction and Fourier transform infrared spectroscopy carried out. These deposits were found to have non-uniform internal structures composed of layers of a structureless aspidinic inorganic material, substantial amounts of voluminous organic material and in a few samples small spheres were also present. Two groups of deposits with distinctly different chemical compositions were identified: one group with a low ca/P molar ratio (1.59) and the other group with a high (1.82) ca/P molar ratio. The deposits belonging to the group with a low ca/P molar ratio contain higher concentration of magnesium and consist of increased amount of amorphous calcium phosphate. The deposits with a high ca/P molar ratio contain low concentration of magnesium and consist predominantly of carbonated hydroxyapatite. The inorganic material was identified as a poorly crystalline carbonate hydroxyapatite containing molecular water of the average formula ca9.1Mg0.4(Na,K)(PO4)5.8(cO3)0.3(OH)2. The actual chemical composition of the apatitic solid phase varies not only from deposit to deposit but also within the same deposit. The non-uniform internal structure of the deposits, the occasional presence of spherical particles and the variable point composition of the individual deposits indicate that their formation did not proceed under more or less constant conditions.

关键词： Mitochondrial Swelling cytochrome c release calcium Alloxan Mitochondrial Permeability Transition

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：