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Proteomic Analysis Shows Constitutive Secretion of MIF and p53-associated Activity of COX-2-/-Lung Fibroblasts

Genomics, Proteomics & Bioinformatics 2017年第6期15卷 339-351页

作者： Mandar Dave Abul B.M.M.K.Islam Roderick V.Jensen Agueda Rostagno Jorge Ghiso Ashok R.Amin Department of Rheumatology New York University Hospital for Joint DiseasesNew YorkNY 10003USA Department of Science STEM DivisionUnion County CollegeCranfordNJ 07016USA Department of Genetic Engineering and Biotechnology University of DhakaDhaka 1000Bangladesh Department of Biological Sciences College of ScienceVirginia TechBlacksburgVA 24060USA Departments of Pathology New York University School of MedicineNew YorkNY 10003USA Department of Bio-Medical Engineering Virginia TechBlacksburgVA 24060USA RheuMatric Inc. BlacksburgVA 24061USA

The differential expression of two closelyassociated cyclooxygenase isozymes, COX-1 and COX-2, exhibited functions beyond eicosanoid metabolism. We hypothesized that COX-1 or COX-2 knockout lung fibroblasts may display altered protein profiles which may allow us to further differentiate the functional roles of these isozymes at the molecular level. Proteomic analysis shows constitutive production of macrophage migration inhibitory factor （MIF） in lung fibroblasts derived from COX-2 / but not wild-type （WT） or COX-1-/- mice. MIF was spontaneously released in high levels into the extracellular milieu of COX2 /- fibroblasts seemingly from the pre- formed intracellular stores, with no change in the basal gene expression of MIF. The secretion and regulation of MIF in COX-2-/- was ＂prostaglandin-independent.＂ GO analysis showed that concurrent with upregulation of MIF, there is a significant surge in expression of genes related to fibroblast growth, FK506 binding proteins, and isomerase activity in COX-2 ! cells. Furthermore, COX-2-/- fibroblasts also exhibit a significant increase in transcriptional activity of various regu- lators, antagonists, and co-modulators of p53, as well as in the expression of oncogenes and related transcripts. Integrative Oncogenomics Cancer Browser （IntroGen） analysis shows downregulation of COX-2 and amplification of MIF and/or p53 activity during development of glioblastomas, ependymoma, and colon adenomas. These data indicate the functional role of the MIF-COX- p53 axis in inflammation and cancer at the genomic and proteomie levels in COX-2-ablated cells. This systematic analysis not only shows the proinflammatory state but also unveils a molecular signature of a pro-oncogenic state of COX-1 in COX-2 ablated cells.

关键词： MIF p53 cyclooxygenases Cancer Proteomics

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