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Effects of plant extract neferine on cyclic adenosine monophosphate and cyclic guanosine monophosphate levels in rabbit corpus cavernosum in vitro

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Asian Journal of Andrology 2008年第2期10卷 307-312页

作者： Jun Chen Ji-Hong Liu Tao Wang Heng-Jun Xiao Chun-Ping Yirl Jun Yang Department of Urology Tongji Hospital Huazhong University of Science and Technology Wuhan 430030 China Department of Urology The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630 China Department of Pharmacy Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Aim： To further investigate the relaxation mechanism of neferine （NED, a bis-benzylisoquinoline alkaloid extracted （isolated） from the green seed embryo of Nelumbo nucifera Gaertn in China, on rabbit corpus cavernosum tissue in vitro. Methods： The effects of Nef on the concentrations of cyclic adenosine monophosphate （cAMP） and cyclic guanosine monophosphate （cGMP） in isolated and incubated rabbit corpus cavernosum tissue were recorded using ^125I radioimmunoassay. Results： The basal concentration of cAMP in corpus cavernosum tissue was 5.67 ± 0.97 pmol/mg. Nef increased the cAMP concentration in a dose-dependent manner （P 〈 0.05）, but this effect was not inhibited by an adenylate cyclase inhibitor （cis-N-[2-phenylcyclopentyl]azacyclotridec-1-en-2-amine, MDL-12, 330A）（P 〉 0.05）. The accumulation of cAMP induced by prostaglandin Et （PGEt, a stimulator of cAMP production） was also augmented by Nef in a dose-dependent manner （P 〈 0.05）. The basal concentration of cGMP in corpus cavernosum tissue is 0.44 ± 0.09 pmol/mg. Nef did not affect this concentration of cGMP, either in the presence or in the absence of a guanyl cyclase inhibitor （1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, ODQ）（P 〉 0.05）. Also, sodium nitroprusside （SNP, a stimulator of cGMP production）-induced cGMP production was not enhanced by Nef （P 〉 0.05）. Conclusion： Nef, with its relaxation mechanism, can enhance the concentration of cAMP in rabbit corpus cavernosum tissue, probably by inhibiting phosphodiesterase activity. （Asian JAndro12008 Mar; 10： 307-312）

关键词： neferine cyclic adenosine monophosphate cyclic guanosine monophosphate rabbit corpus cavernosum

Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate

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International Journal of Oral Science 2013年第2期5卷 85-91页

作者： Jyun-Yi Wu Chia-Hsin Chen Li-Yin Yeh Ming-Long Yeh Chun-Chan Ting Yan-Hsiung Wang Institute of Biomedical Engineering National Cheng Kung University Department of Physical Medicine and Rehabilitation Kaohsiung Medical University Hospital Department of Physical Medicine and Rehabilitation Faculty of MedicineCollege of MedicineKaohsiung Medical University Department of Physical Medicine and Rehabilitation Kaohsiung Municipal Ta-Tung Hospital Department of Oral Hygiene College of MedicineKaohsiung Medical University School of Dentistry College of Dental MedicineKaohsiung Medical University Orthopaedic Research Center College of MedicineKaohsiung Medical University

Retaining or improving periodontal ligament （PDL） function is crucial for restoring periodontal defects. The aim of this study was to evaluate the physiological effects of low-power laser irradiation （LPLI） on the proliferation and osteogenic differentiation of human PDL （hPDL） cells. Cultured hPDL cel Is were irradiated （660 nm） daily with doses of O, 1, 2 or 4 J .cm-2. Cell proliferation was evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide （MTT） assay, and the effect of LPLI on osteogenic differentiation was assessed by Alizarin Red S staining and alkaline phosphatase （ALP） activity. Additionally, osteogenic marker gene expression was confirmed by real-time reverse transcription-polymerase chain reaction （RT-PCR）. Our data showed that LPLI at a dose of 2 ***-2 significantly promoted hPDL cell proliferation at days 3 and 5. In addition, LPLI at energy doses of 2 and 4 ***-2 showed potential osteogenic capacity, as it stimulated ALP activity, calcium deposition, and osteogenic gene expression. We also showed that cyclic adenosine monophosphate （cAMP） is a critical regulator of the LPLI-mediated effects on hPDL cells. This study shows that LPLI can promote the proliferation and osteogenic differentiation of hPDL cells. These results suggest the potential use of LPLI in clinical applications for periodontal tissue regeneration.

关键词： cell proliferation cyclic adenosine monophosphate human periodontal ligament cells low-power laser irradiation osteogenic differentiation

Inhibition of matrix metalloproteinase-9 secretion by dimethyl sulfoxide and cyclic adenosine monophosphate in human monocytes

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World Journal of Biological Chemistry 2021年第1期12卷 1-14页

作者： Darcy R Denner Maria LD Udan-Johns Michael R Nichols Department of Chemistry and Biochemistry University of Missouri-St.LouisSt LouisMO 63121United States

BACKGROUND Matrix metalloproteinases(MMPs),including MMP-9,are an integral part of the immune response and are upregulated in response to a variety of *** details continue to emerge concerning the mechanistic and regulatory pathways that mediate MMP-9 *** is significant evidence for regulation of inflammation by dimethyl sulfoxide(DMSO)and 3',5'-cyclic adenosine monophosphate(cAMP),thus investigation of how these two molecules may regulate both MMP-9 and tumor necrosis factorα(TNFα)secretion by human monocytes was of high *** hypothesis tested in this study was that DMSO and cAMP regulate MMP-9 and TNFαsecretion by distinct *** To investigate the regulation of lipopolysaccharide(LPS)-stimulated MMP-9 and tumor necrosis factorαsecretion in THP-1 human monocytes by dimethyl sulfoxide and *** The paper describes a basic research study using THP-1 human monocyte *** experiments were conducted at the University of *** in the Department of Chemistry and *** monocyte cells were grown,cultured,and prepared for experiments in the University of *** Cell Culture Facility as per accepted *** were treated with LPS for selected exposure times and the conditioned medium was collected for analysis of MMP-9 and TNFα*** including DMSO,cAMP regulators,and anti-TNFαantibody were added to the cells prior to LPS ***-9 secretion was analyzed by gel electrophoresis/western blot and quantitated by ImageJ ***αsecretion was analyzed by enzyme-linked immuno sorbent *** data is presented as the average and standard error for at least 3 *** analysis was done using a two-tailed paired Student t-test.P values less than 0.05 were considered significant and designated as such in the *** and cAMP regulators were from Sigma-Aldrich,MMP-9 standard and antibody and TNFαantibodies were from R&D Systems,and amyloid-βpeptide was fro

关键词： Matrix metalloproteinase-9 Inflammation Human monocytes Tumor necrosis factor alpha cyclic adenosine monophosphate Dimethyl sulfoxide

A New Discovery:Ziziphus jujuba Mill Peel Contains the Highest Amount of cyclic adenosine monophosphate

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A New Discovery:Ziziphus jujuba Mill Peel Contains the Highe...

Effects of Zuogui Pill(左归丸) and Yougui Pill(右归丸) on the Expression of Brain-Derived Neurotrophic Factor and cyclic adenosine monophosphate/Protein Kinase A Signaling Transduction Pathways of Axonal Regeneration in Model Rats with Experimental Autoimm

中国化学会第十九届全国有机分析及生物分析学术研讨会

作者： Fengzheng Chen Department of Chemistry Leshan Normal University

Background:Thefruit of Ziziphus jujuba Mill is atraditionalChinese medicine and *** find optimal Jujube raw material for preparation Jujubefruit oral liquid characterized by the content cyclic adenosine monophosphate（cAMP）,four levels *** and methods:The cAMP contents of four levels Jujubefruit peels were determined by high performance liquid ***:The result showed that the cAMP content of grade A Jujubefruit is lowest and grade D Jujubefruit is highest;what’s more,the cAMP content of peel is about 885 μg/g,which is almost eighty percent higher than the pulp’***:A new discovery:the highest content cAMP is found in Ziziphus jujuba Mill Peel.

关键词： Ziziphus jujuba Mill Jujube fruit cyclic adenosine monophosphate HPLC

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Chinese Journal of Integrative Medicine 2014年第1期20卷 24-30页

作者：王义周寇爽谷凌云郑琦李明齐放赵晖王蕾 School of Traditional Chinese Medicine Capital Medical University Concord Medical Services Holdings Limited Department of Traditional Chinese Medicine the Military General Hospital of Beijing People's Liberation Army

Objective：To study the effects of Zuogui Pill（左归丸,ZGP）and Yougui Pill（右归丸,YGP）on the expressions of brain-derived neurotrophic factor（BDNF）and cyclic adenosine monophosphate（cAMP）/protein kinase A（PKA）signaling of axonal regeneration in the Lewis rats with experimental autoimmune encephalomyelitis（EAE）,in order to explore the possible mechanism of ZGP and YGP on promoting axonal ***：The rats were randomly divided into normal control（NC）,model（MO）,prednisone acetate（PA）,ZGP and YGP *** EAE model of rat was established by injecting antigen containing myelin basic protein（MBP）*** brain and spinal cord were harvested on the 14th and 28th day postimmunization（PI）,the protein and mRNA expression of BDNF and PKA in the brain and spinal cord of rats were detected by Western blot analysis and real-time quantitative polymerase chain reaction（PCR）,and the cAMP levels were detected by using enzyme-immunoassay ***：（1）On the 28th day PI,the mRNA expression of BDNF in brain white matter and spinal cord of rats in ZGP and YGP groups were up-regulated,especially in YGP group（P〈0.05 or P〈0.01）.（2）On the 14th day PI,the cAMP levels in brain white matters significantly increased in PA and YGP groups compared with MO group（P〈0.05 or P〈0.01）,and the cAMP level in YGP group was higher than that in ZGP group（P〈0.05）.The cAMP level in spinal cord also significantly increased in YGP group compared with MO,PA and ZGP groups,respectively（P〈0.01）.（3）On the 14th day PI,the PKA expression in spinal cord of rats in ZGP group was significantly decreased compared with MO and YGP groups,respectively（P〈0.05）.（4）On the 28th day PI,there was a positive correlation between cAMP and PKA expression in the brain white matter of YGP ***：The results suggest that ZGP and YGP may promote axonal regeneration by modulating cAMP/PKA signal transduction pathway,but the targets of molecular mechanism of ZGP may be different from those of YGP.

关键词： experimental autoimmune encephalomyelitis Zuogui Pill Yougui Pill brain-derived neurotrophic factor cyclic adenosine monophosphate protein kinase A axonal injury and regeneration Chinese medicine

Exendin-4 inhibits high-altitude cerebral edema by protecting against neurobiological dysfunction

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Neural Regeneration Research 2018年第4期13卷 653-663页

作者： Zhong-Lei Sun Xian-Feng Jiang Yuan-Chi Cheng Ying-Fu Liu Kai Yang Shuang-Long Zhu Xian-Bin Kong Yue Tu Ke-Feng Bian Zhen-Lin Liu Xu-Yi Chen Affiliated Hospital of Logistics University of Chinese People's Armed Police Forces Institute of Traumatic Brain Trauma and Neurological of CAPF Neurotrauma Repair Key Laboratory of Tianjin Tianjin China Jinzhou Medical University Jinzhou Liaoning Province China Tianjin Medical University Tianjin China Central Hospital of Fengxian District of Shanghai Shanghai China Logistics University of People's Armed Police Force Tianjin China The No. 2 Hospital of Nanjing Nanjing Jiangsu Province China Tianjin University of Traditional Chinese Medicine Tianjin China

The anti-inflammatory and antioxidant effects of exendin-4（Ex-4） have been reported ***,whether（Ex-4） has anti-inflammatory and antioxidant effects on high-altitude cerebral edema（HACE） remains poorly *** this study,two rat models of HACE were established by placing rats in a hypoxic environment with a simulated altitude of either 6000-or 7000-m above sea level（MASL） for 72 *** altitude of 7000 MASL with 72-hours of hypoxia was found to be the optimized experimental paradigm for establishing HACE ***,in rats where a model of HACE was established by introducing them to a 7000 MASL environment with 72-hours of hypoxia treatment,2,10 and,100 μg of Ex-4 was intraperitoneally *** open field test and tail suspension test were used to test animal *** methods were used to detect change in inflammatory ***-eosin staining was performed to determine pathological changes to brain ***/dry weight ratios were used to measure brain water *** blue leakage was used to determine blood-brain barrier ***-linked immunosorbent assay（ELISA） was performed to measure markers of inflammation and oxidative stress including superoxide dismutase,glutathione,and malonaldehyde values,as well as interleukin-6,tumor necrosis factor-alpha,cyclic adenosine monophosphate levels in the brain *** blot analysis was performed to determine the levels of occludin,ZO-1,SOCS-3,vascular endothelial growth factor,EPAC1,nuclear factor-kappa B,and *** results demonstrate that Ex-4 preconditioning decreased brain water content,inhibited inflammation and oxidative stress,alleviated brain tissue injury,maintain blood-brain barrier integrity,and effectively improved motor function in rat models of *** findings suggest that Ex-4 exhibits therapeutic potential in the treatment of HACE.

关键词： high-altitude cerebral edema exendin-4 cyclic adenosine monophosphate EPACI suppressor of cytokine signaling 3 vascular endothelial growth factor hypoxia inflammation oxidative stress

Expression and role of 5-HT7 receptor in brain and intestine in rats with irritable bowel syndrome

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Chinese Medical Journal 2007年第23期120卷 2069-2074页

作者： ZOU Bai-cang DONG Lei WANG Yan WANG Sheng-hao CAO Ming-bo Department of Gastroenterology Second Hospital of Xi＇anJiaotong University Xi＇an 710004 China

Background The 5-hydroxytryptamine7 receptor （5-HT7 receptor, 5-HTTR） plays an important role in the regulation of smooth muscle relaxation and visceral sensation and might be involved in the pathogenesis of the gastrointestinal dyskinesia, abdominal pain and visceral paresthesia in irritable bowel syndrome （IBS）. The aim of this study was to investigate the role of the 5-HT7 receptor in the pathogenesis of IBS. Methods A rat model of irritable bowel syndrome with diarrhea （IBS-D） was established by colonic instillation of acetic acid and restraint stress. A rat model with irritable bowel syndrome with constipation （IBS-C） was established by stomach irrigated with 0-4℃ cool water daily for 14 days. The content and distribution of 5-HT in the brain and gut were examined by immunohistochemistry and the mRNA expression of the 5-HT7 receptor was determined by fluorescent quantitative reverse transcription polymerase chain reaction. The accumulation of cyclic adenosine monophosphate （cAMP） in all the same tissues was measured by radioimmunity. Results The models of IBS were reliable by identification. The immunohistochemistry results showed that there were significantly more 5-HT positive cells in the IBS-D group than in the control group in the hippocampus, hypothalamus, jejunum, ileum, proximate colon and distal colon （P〈0.05）, as well as more than were found in the IBS-C group in jejunum and ileum （P〈0.05）. There were more 5-HT positive cells in the IBS-C group than in the control hippocampus, hypothalamus, ileum, proximate colon, and distal colon （P〈0.05）. Real time-PCR results showed that the expression level of the 5-HT7 receptor in both the IBS-C and IBS-D groups were enhanced compared with the control group in the hippocampus and hypothalamus （P〈0.05）. The expression level of 5-HT7 receptors in the IBS-C group was notably greater when compared with the controls in the ileum and colon （P〈0.05）. The cAMP accumulation in the hippocampus and hypothalamus in both the

关键词： irritable bowel syndrome 5-hydroxytryptamine 5-hydroxytryptamine7 receptor cyclic adenosine monophosphate

adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca^(2+)

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Food Science and Human Wellness 2023年第3期12卷 832-841页

作者： Qiao Bai Xiaoping Feng Yu Wang Chunyu Wu Ye Liu Jiao Sun Tianli Yue Fangyu Long College of Food Science and Engineering Northwest A&F UniversityYangling 712100China Beijing Key Laboratory of Plant Resource Research and Development Beijing Technology and Business UniversityBeijing 100048China Department of Breast Surgery The Affi liated Hospital of Qingdao UniversityQingdao 266000China

Jujube contains abundant cyclic adenosine monophosphate(cAMP)and the ultrasonic-assisted pectinase extraction(UAPE)conditions for obtaining the maximum cAMP yield from jujube were *** array design was applied to evaluate the effects of 4 variables by UAPE on cAMP *** results showed that the optimal cAMP yield(783.0μg/g)was derived at ratio of liquid to solid 5 mL/g,ratio of pectinase to raw material 1.5%,time 60 min and temperature 40℃.Moreover,the effect of cAMP on the anti-allergic function of action induced by immunoglobulin E(IgE)and its meschanism was investigated through establishing the sensitized cell model in rat basophilic leukemia(RBL-2 H3)cells using dinitrophenylated(DNP)-bovine serum albumin(BSA)-*** results showed that cAMP interfered with sensitized cells,effectively inhibited the occurrence of basophil degranulation in dose dependence,and significantly reduced the activity ofβ-hexosamindase(β-hex),at the optimal concentration of 50μg/*** level of anti-inflammatory factor interleukin-10(IL-10)was promoted and the content of pro-inflammatory factor tumor necrosis factor-α(TNF-α)was suppressed by *** addition,influx of intracellular Ca^(2+) was repressed *** results demonstrate that jujube cAMP regulated the cytokine balance in the allergy pathway through blocking the influx of extracellular Ca^(2+),with the prevention of allergy symptoms.

关键词： Ultrasonic-assisted pectinase extraction cyclic adenosine monophosphate Rat basophilic leukemia cells Ca^(2+)influx Allergy

Taurochenodeoxycholic acid mediates cAMP-PKA-CREB signaling pathway

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Chinese Journal of Natural Medicines 2020年第12期18卷 898-906页

作者： QI You-Chao DUAN Guo-Zhen MAO Wei LIU Qian ZHANG Yong-Liang LI Pei-Feng College of Veterinary Medicine Inner Mongolia Agricultural UniversityHohhot 010018China Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease Ministry of AgricultureHohhot 010018China College of Forestry Inner Mongolia Agricultural UniversityHohhot 010018China Immunology Progamme Life Sciences InstituteNational University of SingaporeSingapore 117597Singapore

Taurochenodeoxycholic acid(TCDCA)is one of the main effective components of bile acid,playing critical roles in apoptosis and immune responses through the TGR5 *** this study,we reveal the interaction between TCDCA and TGR5 receptor in TGR5-knockdown H1299 cells and the regulation of inflammation via the cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)-cAMP response element binding(CREB)signal pathway in NR8383 *** TGR5-knockdown H1299 cells,TCDCA significantly activated cAMP level via TGR5 receptor,indicating TCDCA can bind to TGR5;in NR8383 macrophages TCDCA increased cAMP content compared to treatment with the adenylate cyclase(AC)inhibitor ***,activated cAMP can significantly enhance gene expression and protein levels of its downstream proteins PKA and CREB compared with groups of ***,TCDCA decreased tumour necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6,IL-8 and IL-12 through nuclear factor kappa light chain enhancer of activated B cells(NF-κB)*** and CREB are primary regulators of anti-inflammatory and immune *** results thus demonstrate TCDCA plays an essential anti-inflammatory role via the signaling pathway of cAMP-PKA-CREB induced by TGR5 receptor.

关键词： Taurochenodeoxycholic acid TGR5 receptor cyclic adenosine monophosphate Protein kinase A cAMP response element binding Anti-inflammation