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Extracts from Huangqi(Radix Astragali Mongoliciplus) and Ezhu(Rhizoma curcumae Phaeocaulis) inhibit Lewis lung carcinoma cell growth in a xenograft mouse model by impairing mitogen-activated protein kinase signaling, vascular endothelial growth factor prod

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Journal of Traditional Chinese Medicine 2019年第4期39卷 559-565页

作者： Xu Chengyong Wang Yuguo Feng Jian Xu Ran Dou Yongqi Medical School of Chinese People's Liberation Army Chinese People's Liberation Army General HospitalBeijing 100853China Department of Traditional Chinese Medicine Hainan Branch of Chinese People's Liberation Army General HospitalSanyaHainan province 572013China Medical School of Chinese People's Liberation Army Chinese People's Liberation Army General HospitalBeijing 100853China Department of Traditional Chinese Medicine Chinese People's Liberation Army General HospitalBeijing 100853China

OBJECTIVE: To study the anti-tumor effects of the extracts from Huangqi(Radix Astragali Mongolici)and Ezhu(Rhizoma curcumae Phaeocaulis) on the growth of Lewis lung carcinoma(LLC) in a xenograft mouse model and to investigate the possible underlying ***: LLC tumor-bearing C57 BL/6 mice were treated with normal saline, cisplatin(2 mg/kg intraperitoneally every other day), or Huangqi(Radix Astragali Mongolici) and Ezhu(Rhizoma curcumae Phaeocaulis)(1∶1, 2∶1, or 3∶1 ratio;5, 8, or 11 g/kg crude drug intragastrically every day) for 15 *** weights and tumor volumes were measured every other day. Tumors were excised on day 15 and analyzed. Tumor microvessel density(MVD)was assessed by immunohistochemical staining of CD34;and expression of vascular endothelial cell growth factor(VEGF), the mitogen-activated protein kinases p38 mitogen-activated protein kinase(MAPK), extracellular signal-regulated kinases 1 and 2(ERK1/2), and Jun N-terminal kinase(JNK)and their phosphorylated forms were assessed by Western ***: Treatment with cisplatin caused a significant loss of body weight compared with controls,whereas Huangqi(Radix Astragali Mongolici) and Ezhu(Rhizoma curcumae Phaeocaulis) extract combinations had no effect. Extracts from Huangqi(Radix Astragali Mongolici) and Ezhu(Rhizoma curcumae Phaeocaulis) significantly decreased tumor weight and tumor MVD compared with controls,and at the 3∶1 treatment group had similar efficacy to cisplatin in reducing MVD. Tumors from Huangqi(Radix Astragali Mongolici) and Ezhu(Rhizoma curcumae Phaeocaulis) treatments also showed decreased p38 MAPK, p-p38 MAPK, ERK1/2, p-ERK1/2,JNK, and p-JNK expression compared with the control group(all P curcumae Phaeocaulis) hindered LLC growth in the xenograft mous

关键词： Carcinoma,Lewis lung Vascular endothelial cell growth factors Extracellular signal-regulated MAP kinases Angiogenesis Huangqi(Radix Astragali Mongolici) Ezhu(Rhizoma curcumae Phaeocaulis)

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Effect of optimal combination of Huangqi(Radix Astragali Mongolici) and Ezhu(Rhizoma curcumae Phaeocaulis) on proliferation and apoptosis of A549 lung cancer cells

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Journal of Traditional Chinese Medicine 2018年第3期38卷 351-358页

作者： Xu Chengyong Wang Yuguo Feng Jian Qin Li Xu Ran Dou Yongqi Medical School of Chinese People's Liberation Army Chinese People's Liberation Army General Hospital Beijing 100853 China Department of Traditional Chinese Medicine Hainan Branch of Chinese People's Liberation Army General Hospital Sanya 572013 China Department of Traditional Chinese Medicine Chinese People's Liberation Army General Hospital Beijing100853 China

OBJECTIVE: To investigate the effect of optimal combination(E) of Huangqi(Radix Astragali Mongolici) and Ezhu(Rhizoma curcumae Phaeocaulis) on proliferation and apoptosis of A549 lung cancer cells and the possible mechanism underpinning the ***: A uniform design method was used to optimize the E of Huangqi(Radix Astragali Mongolici) and Ezhu(Rhizoma curcumae Phaeocaulis) in A549 lung cancer cells. MTS assay was applied to analyze the effect of the component formula ofHuangqi(Radix Astragali Mongolici) and Ezhu(Rhizoma curcumae Phaeocaulis) on A549 cells viability in various uniform design groups. A549 cells with exponential growth in routine culture were exposed to Co Cl_2(200 μmol/L) to mimic hypoxic conditions. Group 0 was treated with RPMI-1640, the group Co Cl_2 was treated with Co Cl_2(200 μmol/L),the group DDP + Co Cl_2 was treated with 4 mg/L Cisplatin injection(DDP) + Co Cl_2(200 μmol/L), and the drug group was treated with various dose of E(0.5 E, 1 E, 2 E) + Co Cl_2(200 μmol/L). All groups were cultured for 24 h. Cell apoptosis was measured by Annexin V-FITC/propidium iodide double staining and flow cytometry. Western blot assay and quantitative real-time polymerase chain reaction(q RT-PCR) were employed to detect the protein and m RNA expression of B-celllymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax) and cysteinyl aspartate specific proteinase-3(caspase-3).RESULTS: The E obtained by the uniform design was comprise of 200 mg/L Astragalus polysaccharide(X1) and 32 mg/L Curcumin(X3). Group DDP+Co Cl_2, group 1 E + Co Cl_2 and group 2 E + Co Cl_2 promoted the apoptosis of A549 cells(P 0.05). Compared with group 0, various doses of E + Co Cl_2 could up-regulate the expression of Bax and caspase-3 and down-regulate the expression of Bcl-2 at protein and m RNA levels(P < 0.05).CONCLUSION: Astragalus polysacchari

关键词： Lung neoplasms A549 cells Apoptosis Astragalan Huangqi (Radix Astragali Mongolici) Ezhu (Rhizoma curcumae Phaeocaulis) Curcumin Hypoxia

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Advances in studies on anti-cancer mechanism of Rhizome curcumae

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Precision Medicine Research 2019年第4期1卷 103-108页

作者： Jin-Feng Liu Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin 300171China.

The anti-cancer mechanism of Rhizome curcumae mainly includes inhibiting the proliferation of tumor cells,promoting the apoptosis of tumor cells,inhibiting tumor angiogenesis,increasing the effect of chemotherapy drugs and reducing its side effects,reversing tumor multidrug resistance and improving *** paper summarizes and describes the extracts and anticancer actives of Rhizome curcumae in recent years,as well as their anticancer *** provides theoretical basis and ideas for the research of Rhizome curcumae in the field of anticancer.

关键词： Rhizome curcumae Rhizome curcumae oil Anti-tumor Research progress

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贵州南北盘江地区姜黄挥发油成分的气相色谱-质谱联用分析

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安徽农业科学 2012年第10期40卷 5898-5898,5900页

作者：叶世芸黄勇其骆红梅徐文芬贵阳中医学院贵州贵阳550002

[目的]分析贵州南北盘江地区姜科植物姜黄挥发油的化学成分。[方法]用水蒸气蒸馏法提取姜黄挥发油,并用气相色谱-质谱联用(GC-MS)法对挥发油成分进行分析。[结果]共分离了68个峰,确认了其中14个化合物,含量最多的是芳姜黄酮(28.17%)。[... 详细信息

[目的]分析贵州南北盘江地区姜科植物姜黄挥发油的化学成分。[方法]用水蒸气蒸馏法提取姜黄挥发油,并用气相色谱-质谱联用(GC-MS)法对挥发油成分进行分析。[结果]共分离了68个峰,确认了其中14个化合物,含量最多的是芳姜黄酮(28.17%)。[结论]该试验为贵州南北盘江地区姜黄植物药材的品质评价提供科学的参考依据。

关键词：姜黄(RHIZOMA curcumae LONGAE) 挥发油化学成分气相色谱-质谱联用分析

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三七及其混伪品的紫外谱线组法鉴别研究

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安徽农业科学 2012年第9期40卷 5161-5163页

作者：李海涛徐红欣张娜郝丽静河北大学中医学院河北保定071000 保定市青年路幼儿园河北保定071000 河北大学药学院河北省药物质量研究重点实验室河北保定071000

[目的]建立鉴别三七及其混伪品高良姜、白及、姜黄和莪术的紫外谱线组鉴别方法。[方法]采用水、乙醇、石油醚、乙酸乙酯和氯仿对5种药材分别进行提取,然后进行紫外检测及其一阶导数光谱检测。[结果]正品三七与高良姜、白及、姜黄和莪术... 详细信息

[目的]建立鉴别三七及其混伪品高良姜、白及、姜黄和莪术的紫外谱线组鉴别方法。[方法]采用水、乙醇、石油醚、乙酸乙酯和氯仿对5种药材分别进行提取,然后进行紫外检测及其一阶导数光谱检测。[结果]正品三七与高良姜、白及、姜黄和莪术的紫外吸收光谱的峰位置和吸光度有明显的差异。[结论]该方法简便、准确、灵敏,能够很好的区别三七及其混伪品。

关键词：三七(RADIX NOTOGINSENG) 高良姜(RHIZOMA ALPINIAE OFFCINARUM) 白及(RHIZOMA BLETILLAE) 姜黄(RHIZOMA curcumae LONGAE) 莪术(RHIZOMA curcumae). 紫外谱线组法鉴别

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