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Endothelial progenitor cell differentiation using cryopreserved,umbilical cord blood-derived mononuclear cells

Acta Pharmacologica Sinica 2007年第3期28卷 367-374页

作者： Jun-ho JANG~(2,5) Sun-kyung KIM~(3,5) Jeong-eun CHOI~3 Young-jin KIM~3 Hyun-woo LEE~2 Seok-yun KANG~2 Joon-seong PARK~2 Jin-hyuk CHOI~2 Ho-yeong LIM~4 Hugh C KIM~(2,6)2 Department of Hematalogy-Oncology,Ajou University,School of Medicine,Suwon 442749,Korea 3 Biomedical Research Institute,LifeCord,Suwon 442749,Korea 4 Division of Hematology-Ontology,Department of Medicine,Samsung Medical Center,Sungkyunkwan University School of Medicine,Seoul 135710,Korea Department of Hematology-Oncology Ajou University School of Medicine Suwon Korea Department of Biomedical Research Institute Suwon Korea Division of Hematology-Oncology Department of Medicine Samsung Medical Center Sungkyunkwan University School of Medicine Seoul Korea

Aim:To investigate the endothelial differentiation potentiality of umbilical cordblood(UCB),we induced the differentiation of endothelial progenitor cells(EPC)from cryopreserved UCB-derived mononuclear cells(MNC).Methods:MNC fromcryopreserved UCB and peripheral blood(PB)were cultured in M199 medium withendothelial cell growth supplements for 14 *** were characterized by RT-PCR,flow cytometry,and immunocytochemistry *** proliferation of differen-tiated EPC was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide(MTT)assay,and vascular endothelial growth factor(VEGF)concentra-tion was measured using an ELISA *** of UCB-derived EPC werecompared with those of PB-derived ***:A number of round-shapedcells were loosely attached to the bottom after 24 h culture,and numerous spindle-shaped cells began to appear from the round-shaped ones on d *** cellsexpressed endothelial markers such as,Flt-1/VEGFR-1,ecNOS,VE-cadherin,vonWillebrand factor,and secreted VEGE The patterns of endothelial markers of EPCfrom PB and UCB did not show striking *** results of the prolifera-tion and secretion of VEGF were also ***:We successfully cul-tured UCB cells stored at -196℃ into cells with the quality of endothelial *** EPC could be used for angiogenic therapeutics by activating adjacentendothelial cells and enhancing angiogenesis.

关键词： cord blood endothelial cells cryopreserved

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Viable cryopreserved umbilical tissue (vCUT) reduces post-operative adhesions in a rabbit abdominal adhesion model

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Bioactive Materials 2019年第1期4卷 97-106页

作者： Sandeep Dhall Turhan Coksaygan Tyler Hoffman Matthew Moorman Anne Lerch Jin-Qiang Kuang Malathi Sathyamoorthy Alla Danilkovitch Osiris Therapeutics Inc.7015 Albert Einstein DrColumbiaMD21046USA University of Maryland 655 W.Baltimore StreetBaltimoreMD21201USA

Post-operative adhesions,a common complication of surgery,cause pain,impair organ functionality,and often require additional surgical *** of inflammation,protection of injured tissue,and rapid tissue repair are critical for adhesion *** barriers are biomaterials used to prevent adhesions by physical separation of opposing injured *** adhesion barriers have poor anti-inflammatory and tissue regenerative *** cord tissue(UT),a part of the placenta,is inherently soft,conforming,biocompatible,and biodegradable,with antimicrobial,anti-inflammatory,and antifibrotic properties,making it an attractive alternative to currently available adhesion *** use of fresh tissue is preferable,availability and short storage time limit its clinical use.A viable cryopreserved UT(vCUT)“point of care”allograft has recently become *** retains the extracellular matrix,growth factors,and native viable cells with the added advantage of a long shelf life at−80℃.In this study,vCUT's anti-adhesion property was evaluated in a rabbit abdominal adhesion *** cecum was abraded on two opposing sides,and vCUT was sutured to the abdominal wall on the treatment side;whereas the contralateral side of the abdomen served as an internal untreated *** and histological evaluation was performed at 7,28,and 67 days *** adhesions were detectable on the vCUT treated side at all time *** scores for adhesion,inflammation,and fibrosis were lower on the vCUT treated side as compared to the control *** conclusion,the data supports the use of vCUT as an adhesion barrier in surgical procedures.

关键词： Placental cryopreserved Post-surgical Adhesiolysis Inflammation Fibrosis

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Long-Term Structural Valve Degeneration in cryopreserved Mitral and Aortic Homograft: Is Pregnancy a Factor?

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World Journal of Cardiovascular Diseases 2014年第6期4卷 277-286页

作者： Francesco Nappi Cristiano Spadaccio Massimo Chello Mario Lusini Cristophe Acar Department of Cardiac Surgery Centre Cardiologique du Nord St. Denis France

Objective: This study reports the outcomes of the cryopreserved mitral homograft in 119 patients prospectively followed with clinical, echocardiographic and structural valve deterioration assessments. Methods: 119 patients undergoing mitral and aortic homograft implantation. Patient’s causes of mitral disease were: rheumatic disease (n = 75), endocarditis (n = 37) and others (n = 7). There were 40 partial homografts and 88 total homografts. Mitro-aortic homograft valve replacement was performed in 29 cases. Results: Mean follow-up was 9.8 ± 6.3 years (up to 19.2 yrs). There were 7 early (2

关键词： Mitral Homograft cryopreserved

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