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Effects of Extreme Air Temperature and Humidity on the Insecticidal Expression Level of Bt Cotton

Journal of Integrative Agriculture 2012年第11期11卷 1836-1844页

作者： CHENYuan WEN Yu-jin CHEN Yuan John Tom Cothren ZHANG Xiang WANG Yong-hui WilliamA Payne CHEN De-hua Ecology and Cultivation in Middle and Lower Reaches of Yangtse River Ministry of Agriculture/Agricultural College Yangzhou University Yangzhou 225009 P.R.China Department of Soil and Crop Sciences Texas A&M University College Station TX77843 USA Key Laboratory of Crops Genetics and Physiology Jiangsu Province/Agricultural College Yangzhou University Yangzhou 225009 P.R. China Norman Borlaug Institute for International Agriculture Texas A&M University College Station TX77843 USA

The higher survival rates of Helicoverpa amigera larvae were usually observed after adverse climate which was related to extreme temperature （T） and relative humidity （RH） stresses in transgenic Bacillus thuringiensis （Bt） cotton. The unstable resistance of Bt cotton to bollworms has been correlated with the reduced expression of CrylAc δ-endotoxin. The objective of this study was to investigate the effects of combined temperature and relative humidity stresses on the leaf CrylAc insecticidal protein expression during critical developmental stages. The study was undertaken on two transgenic cotton cultivars that share same parental background, Sikang 1 （a conventional cultivar） and Sikang 3 （a hybrid cultivar）, during the 2007 and 2008 growing seasons at the Yangzhou University Farm, Yangzhou, China. The study was arranged with two factors that consisted of temperature （two levels） and relative humidity （three levels）. The six T/RH treatments were 37℃/95%, 37℃/70%, 37℃/50%, 18℃/95%, 18℃/70%, and 18℃/50%. In 2007, the six treatments were imposed to the plants at peak flowering stage for 24 h; in 2008, the six treatments were applied to the plants at peak square, peak flowering, and peak boll stages for 48 h. The results of the study indicated that the leaf insecticidal protein expression in CrylAc was significantly affected by extreme temperature only at peak flowering stage, and by both extreme temperature and relative humidity during boll filling stage. The greatest reductions were observed when the stresses were applied at peak boll stage. In 2008, after 48 h stress treatment, the leaf Bt endotoxin expression reduced by 25.9-36.7 and 23.6-40.5% at peak boll stage, but only by 14.9-26.5 and 12.8-24.0% at peak flowering stage for Sikang 1 and Sikang 3, respectively. The greatest reduction was found under the low temperature combined with low relative humidity condition for both years. It is believed that the temperature and relative humidity stresses may be attribut

关键词： Bt cotton high temperature low temperature relative humidity cryiac protein

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