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Screening of stable internal reference genes by quantitative real-time PCR in humpback grouper cromileptes altivelis

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Journal of Oceanology and Limnology 2021年第5期39卷 1985-1999页

作者： Xiaojuan CHEN Yun SUN Panpan ZHANG Jianlong LI Haiping LI Caoying WEI Zhenjie CAO Yongcan ZHOU State Key Laboratory of Marine Resource Utilization in South China Sea Hainan UniversityHaikou 570228China Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province Haikou 570228China Department of Aquaculture College of Marine SciencesHainan UniversityHaikou 570228China

Humpback grouper cromileptes altivelis is one commercial fish with considerable economic *** determine the expression stabilities of six commonly used internal reference genes in *** challenged by Vibrio harveyi and viral nervous necrosis virus(VNNV)through quantitative real-time PCR(qRT-PCR),the expression levels of selected genes in five immune organs stimulated with pathogenic infection were carefully evaluated using algorithms of geNorm,NormFinder,and *** results show that the expre ssion stabilities of the six candidate inte rnal reference genes were diffe re *** no rmal physiological conditions,RPL13 were identified as the most stably expressed genes among five different immune organs(liver,spleen,kidney,intestine,and gill).After *** stimulation,RPL13,RPL13,EF1 A,RPL13,and EF1 A were identified by geNorm,NormFinder,and BestKeeper as the most stable genes in liver,spleen,kidney,intestine,and gill,*** these three algorithms suggested that under stimulation of VNNV,RPL13,EF1 A,Actin,RPL13,and Actin were as the most stable genes in liver,spleen,kidney,intestine,and gill,*** results suggest that specific experiment conditions and tissue types shall be considered when selecting the reference genes in qRT-PCR *** study provided a solid foundation for future studies on gene expression of *** under different conditions.

关键词： cromileptes altivelis reference gene expression stability pathogenic infection

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