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creatine kinase in the diagnosis and prognostic prediction of amyotrophic lateral sclerosis:a retrospective case-control study

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Neural Regeneration Research 2021年第3期16卷 591-595页

作者： Xue-Ping Chen Qian-Qian Wei Ru-Wei Ou Yan-Bing Hou Ling-Yu Zhang Xiao-Qin Yuan Yun-Qian Yao De-Sheng Jia Qian Zhang Wei-Xue Li Hui-Fang Shang Department of Neurology West China Hospital of Sichuan UniversityChengduSichuan ProvinceChina

creatine kinase is a muscle enzyme that has been reported at various levels in different studies involving patients with amyotrophic lateral *** the present retrospective case-control study,we included 582 patients with amyotrophic lateral sclerosis and 582 age-and sexmatched healthy *** amyotrophic lateral sclerosis participants received treatment in the Department of Neurology,West China Hospital,China,between May 2008 and December *** creatine kinase levels in patients with amyotrophic lateral sclerosis were significantly higher than those in healthy *** analysis revealed that serum creatine kinase levels in men were higher than those in women in both amyotrophic lateral sclerosis patients and healthy *** with patients with bulbar-onset amyotrophic lateral sclerosis,patients with limb-onset amyotrophic lateral sclerosis had higher creatine kinase ***'s correlation analysis revealed that serum creatine kinase levels were not correlated with body mass index,Amyotrophic lateral Sclerosis Functional Rating ScaleRevised score,or progression *** adjusting for prognostic covariates,higher log creatine kinase values were correlated with higher overall survival in the amyotrophic lateral sclerosis *** also investigated the longitudinal changes in serum creatine kinase levels in 81 amyotrophic lateral sclerosis patients;serum creatine kinase levels were decreased at the second blood test,which was sampled at least 6 months after the first blood ***,our results suggest that serum creatine kinase levels can be used as an independent factor for predicting the prognosis of amyotrophic lateral sclerosis *** study received ethical approval from the Ethics Committee of West China Hospital,China(approval No.2015(236))on December 23,2015.

关键词： amyotrophic lateral sclerosis biomarker creatine kinase diagnosis epidemiological study prognosis serology survival

American ginseng supplementation attenuates creatine kinase level induced by submaximal exercise in human beings

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World Journal of Gastroenterology 2005年第34期11卷 5327-5331页

作者： Cheng-Chen Hsu Min-Chen Ho Li-Chin Lin Borcherng Su Mei-Chich Hsu Graduate Institute of Physical Education National College of Physical Education and Sports Taoyuan County and School of Medicine Taipei Medical University Taipei Taiwan China Graduate Institute of Sports Science National College of Physical Education and Sports Taoyuan County Taiwan China Taiwan Biotech Co. Ltd Taiwan China Department of Anatomic Pathology E-Da Hospital I-Shou University Kaoshiung County Taiwan Republic of China

AIM: To investigate whether American ginseng (AG, Panax quinquefolium) supplementation was able to improve endurance exercise ***: Thirteen physically active male college students were divided into two groups (AG or placebo)and received supplementation for 4 wk, before the exhaustive running exercise. Treadmill speed was increased to a pace equivalent to 80% VO2max of the subject. A 4-wk washout period followed before the subjects crossed over and received the alternate supplement for the next 4 *** then completed a second exhaustive running exercise. The physiological variables that were examined included time to exhaustion and oxygen pulse. Moreover,the plasma creatine kinase (CK) and lactate were measured prior to the exercise, at 15 and 30 min during exercise,immediately after exercise, and 20, 40, 60, and 120 min after ***: The major finding of this investigation was that the production plasma CK during the exercise significantly decreased for group AG than for group P. Secondary physiological finding was that 80% VO2max running was not improved over a 4-wk AG supplementation ***: Supplementation with AG for 4 wk prior to an exhaustive aerobic treadmill running reduced the leakage of CK during exercise, but did not enhance aerobic work capacity. The reduction of plasma CK may be due to the fact that AG is effective for the decrease of skeletal muscle cell membrane damage, induced by exercise during the high-intensity treadmill run.

关键词： Panax quinquefoflum American ginseng creatine kinase Endurance exercise

creatine kinase profiling with adolescent sprinters and jumpers-an approach based on individual reference ranges

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Creatine kinase profiling with adolescent sprinters and jump...

Studies on the creatine kinase MM isoforms of normal and Duchenne muscular dystrophic patients

第16届亚洲运动会科学大会

作者： Voss SC Elzain Elgingo MI Sailly M Mattar R Merzoian A Peltola E Bourdon PASPIRE Academy Sport Science Directorate

＜正＞Introduction:The concentration of creatine kinase（CK）in blood has been widely used for many years as a marker of exercise induced muscle *** values for male and female athletes have previously been established... 详细信息

ISBN: (纸本)9787564405564

关键词： reference value Athletes creatine kinase Serum levels Adolescent responders

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Chinese Medical Journal 1998年第1期111卷 75-77页

作者：赵昕毕春雷杨振华 Department of Laboratory Medicine Beijing Hospital Beijing 100730 China

OBJECTIVE: To study the changes of creatine kinase MM (CK-MM) isoforms in Duchenne muscular dystrophy (DMD) patients. METHODS: Serum samples from 49 DMD patients and 40 control subjects were collected for CK-MM isoforms measurement. CK-MM isoforms were separated within 30 minutes by electrophoresis on agarose gel with a discontinuous buffer system at constant current of 30 mA and low voltage of 200-300 V, then measured by fluorescence scanning. RESULTS: Significant differences of MM2/MM1 ratio were found between DMD patients and control subjects (P

关键词： Adolescent Adult Biological Markers Child Child, Preschool creatine kinase Electrophoresis, Agar Gel Female Humans Isoenzymes Male Muscular Dystrophies

Effects of Tuina on serum creatine kinase and skeletal muscle mitochondria in delayed onset muscle soreness model rats

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Journal of Acupuncture and Tuina Science 2022年第6期20卷 446-452页

作者： WEI Qingbo ZHAO Qian GU Jialing LIN Jia ZHU Yan SONG Ziqi LI Fenglei YANG Yanping Nanjing University of Chinese Medicine Nanjing 210023China Jiangsu Second Chinese Medicine Hospital Nanjing 210017China Jiangsu Haibin Rehabilitation Hospital Lianyungang 222042China 不详

Objective To observe the effect of Tuina(Chinese therapeutic massage)on creatine kinase(CK),mitochondrial Ca^(2+)concentration,and ultrastructure of skeletal muscle in delayed onset muscle soreness(DOMS)model *** A total of 130 healthy male Sprague-Dawley rats were randomly divided into a blank group,an exercise control group,a pre-exercise Tuina group,and a post-exercise Tuina *** to the time points for sample collection,the exercise control group was divided into a 0 h exercise control group,a 24 h exercise control group,a 48 h exercise control group,and a 72 h exercise control group;the pre-exercise Tuina group was further divided into a 0 h pre-exercise Tuina group,a 24 h pre-exercise Tuina group,a 48 h pre-exercise Tuina group,and a 72 h pre-exercise Tuina group;and the post-exercise Tuina group was divided into a 0 h post-exercise Tuina group,a 24 h post-exercise Tuina group,a 48 h post-exercise Tuina group,and a 72 h post-exercise Tuina *** in all groups except for the blank group received DOMS *** performed Nie-Pinching manipulation and finger Nian-Twisting manipulation on the lower limbs of the *** samples were collected at 0 h,24 h,48 h,or 72 h after exhaustive exercise for each pre-exercise Tuina *** samples were collected at 0 h,24 h,48 h,or 72 h after Tuina for each post-exercise Tuina *** changes in serum CK,skeletal muscle mitochondrial Ca^(2+)concentration,and Ca^(2+)-adenosine triphosphatase(ATPase)were *** ultrastructure changes of skeletal muscles in each group were observed by a transmission electron *** The electron microscope showed that compared with the exercise control group,the skeletal muscle structures of the pre-exercise Tuina group and the post-exercise Tuina group were significantly improved,and the overall performance of skeletal muscle in the pre-exercise Tuina group was more similar to that of the blank *** level of serum CK in the pre-ex

关键词： Tuina Massage Manual Therapies creatine kinase Myalgia Fatigue Mitochondria Rats

Effects of Glycerol in the Refolding and Unfolding of creatine kinase

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Tsinghua Science and Technology 2002年第4期7卷 352-362页

作者：欧文斌朴龙斗孟凡国周海梦 Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

The effects of glycerol in the refolding, reactivation, unfolding, and inactivation of guanidine denatured creatine kinase were studied by observing the fluorescence emission spectra and the circular dichroism spectra, and by recovery and inactivation of enzymatic activity and aggregation. The results show that low concentrations of glycerol (creatine kinase, but high glycerol concentrations decrease its recovery. Glycerol favors the secondary structural formation and inhibits aggregation of creatine kinase as proline does. These systematic observations further support the suggestion that low concentrations of glycerol possibly play a chaperone role in the refolding of creatine kinase. In addition, glycerol reduces the inactivation and unfolding rate of creatine kinase, increases the change in transition free energy of unfolding (ΔΔG u) and stabilizes its active conformation relative to the partially unfolded state with no glycerol. In the presence of glycerol, the inactivation and unfolding dynamics of creatine kinase are related to glycerol concentrations. Glycerol blocks the exposure of hydrophobic areas and the dissociation of dimers, and protects creatine kinase against guanidine denaturation in a concentration dependent manner. This study suggests that glycerol as an energy substrate for metabolism and organic components in vivo, assists correct protein folding, maintains adequate rates of enzymatic catalysis and stabilizes the protein secondary and tertiary conformations. 

关键词： glycerol creatine kinase unfolding refolding guanidine denaturation

Characterization of the Partially Folded Monomeric Intermediate of creatine kinase

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Tsinghua Science and Technology 2002年第4期7卷 347-351页

作者：朴龙斗周海梦 Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

The importance of understanding the protein folding pathway and intermediates is well recognized on the basis of extensive studies of protein folding in vitro and in vivo. creatine kinase (CK) is a typical model for studying unfolding and refolding of proteins due to several interesting properties. Recent studies on the folding of CK show that its partially folded monomeric intermediate is present kinetically and is stable at equilibrium. The present paper contains 33 references as a mini review to characterize the properties of CK from studies on the CK folding pathway. Characterization of these intermediates is an essential step toward understanding the mechanism of protein folding. Some well determined schemes are suggested as protein folding models.

关键词： creatine kinase intermediate partially folded state folding pathway

Effect of Mixing Macromolecular Crowding Agents on the Refolding of Lysozyme and creatine kinase

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Effect of Mixing Macromolecular Crowding Agents on the Refol...

Serum creatine kinase and high grade glioma-associated seizures

第七届全国酶学学术讨论会

作者： Bing-Rui Zhou, Fen Du, Zheng Zhou, Zhong-Ying Mo, Yi Liang (Laboratory of Structural Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China, E-mail: liangyi@***)

In vivo the main question that protein folding encountered is that theintracellular environment is highly crowded due to the presence of highconcentrations of soluble and insoluble macromolecules,which include protein,nucleic acid,and *** order to mimic the crowding environments in thecytoplasm,we add both protein crowding agent and polysaccharide to the refolding

关键词： macromolecular crowding mixing crowding agents protein refolding lysozyme creatine kinase refolding kinetics

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Serum creatine kinase and high grade glioma-associated seizu...