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Molecular Epidemiology of coxsackievirus B1-5 Associated with HFMD in Fujian Province, China, 2011-2016

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Biomedical and Environmental Sciences 2019年第8期32卷 633-638页

作者： CHEN Wei WENG Yu Wei ZHANG Yong Jun HE Wen Xiang ZHU Ying YU Ting Ting XIE Jian Feng ZHENG Kui Cheng YAN Yan Sheng ZHANG Wen Chang Fujian Centre for Disease Control and Prevention Fujian Provincial Key Laboratory of Zoonosis ResearchFuzhou 350001FujianChina School of Public Health Fujian Medical UniversityFuzhou 350108FujianChina

Hand,foot and mouth disease(HFMD)is a common infectious disease that usually affects children less than 5 years of *** is caused by human enteroviruses(HEVs).HEVs,members of the Enterovirus genus of the Picornaviridae... 详细信息

关键词： Molecular Epidemiology coxsackievirus B1-5 Associated HFMD

coxsackievirus B infection presenting as a hemorrhagic pericardial effusion causing tamponade

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Journal of Geriatric Cardiology 2020年第10期17卷 642-644页

作者： Fadi Kandah Pooja Dhruva Jose Ruiz Andres Martinez William Kogler Internal Medicine University of Florida Health JacksonvilleJacksonvilleUSA

The coxsackievirus is well known for its vastly differing clinical *** with coxsackievirus usually present with a viral prodrome which can then progress to the cardiac symptoms of chest pain and/or *** patients improv... 详细信息

关键词： Cardiac tamponade coxsackievirus Pericarditis

Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo

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Virologica Sinica 2023年第4期38卷 549-558页

作者： Shangrui Guo Meng Xun Tingting Fan Xinyu Li Haoyan Yao Xiaozhen Li Bo Wu Hang Yang Chaofeng Ma Hongliang Wang Department of Pathogen Biology and Immunology Xi’an Jiaotong University Health Science CenterXi’an710061China Department of Gynecology and Obstetrics The First Affiliated Hospital of Xi'an Jiaotong UniversityXi’an710061China Department of Viral Diseases Laboratory Xi’an Center for Disease Control and PreventionXi’an710061China

coxsackievirus belongs to the Picornaviridae family and is one of the major pathogens that cause hand,foot and mouth disease(HFMD)in infants and children with potential serious complications and even *** pathogenesis of this virus is not fully elucidated and no vaccine or antiviral drug has been *** this study,a full-length infectious cDNA clone of coxsackievirus B5 virus was assembled and the recombinant virus displayed similar growth kinetics and ability to cause cytopathic effects as the parental *** reporter was then incorporated to generate both full-length and subgenomic replicon(SGR)reporter *** full-length reporter virus is suitable for high-throughput antiviral screening,while the SGR is a useful tool to study viral-host *** importantly,the full-length reporter virus has also been shown to infect the suckling mouse model and the reporter gene could be detected using an in vivo imaging system,thus providing a powerful tool to track viruses in *** summary,we have generated coxsackievirus B5 reporter viruses and provided unique tools for studying virus-host interactions in vitro and in vivo as well as for high-throughput screenings(HTS)to identify novel antivirals.

关键词： coxsackievirus Luciferase High-throughput screening Mouse model

Molecular Characterisation of Two coxsackievirus B6 Strains from the Tibet Autonomous Region of China

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Biomedical and Environmental Sciences 2019年第9期32卷 699-703页

作者： SUN Qiang HONG Mei FENG Ning ZHANG Yong WHO WPRO Regional Polio Reference Laboratory NHC Key Laboratory of biosafetyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Tibet Center for Disease Control and Prevention Lhasa 850000Tibet Autonomous RegionChina Center for Global Public Health Chinese Center for Disease Control and PreventionBeijing 102206China

Enteroviruses (EVs) are members of the genus Enteroviruswithin the orderPicornavirales, family Picornaviridae, and consist of 12 species, including EV-A, EV-B, EV-C, and EV-D, which are associated with human infections. coxsackievirus B6 (CV-B6)belongs to the species EV-B, which currently consists of 63 serotypes, including echovirus (serotypes 1–7,9, 11–21, 24–27, 29–33), coxsackievirus group A (CVA9), coxsackievirus group B (CV-B, serotypes 1–6),the newly identified EVs (serotypes EV-B69, B73–75,B77–88, B93, B97–98, B100–101, B106–107, and B110–113), and the simian enterovirus SA5.

关键词： coxsackievirus B6 belongs

Molecular Characterization of coxsackievirus B1 Strains Isolated from Patients with Hand Foot and Mouth Disease in Yunnan,Southwest China

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Biomedical and Environmental Sciences 2024年第5期37卷 543-548页

作者： XU Dan Han ZHANG Ming CHEN Jun Wei Feng Chang Zeng LIU Yu Han CHU Zhao Yang LIU Wen Jing LI Li MA Shao Hui Institute of Medical Biology Chinese Academy of Medical Sciencesand Peking Union Medical CollegeKunming 650118YunnanChina Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease Kunming 650118YunnanChina School of Life Sciences Yunnan UniversityKunming 650504YunnanChina School of Medicine Yunnan UniversityKunming 650504YunnanChina Department of Clinical Laboratory Kunming Maternal and Child Health hospital Kunming 650031YunnanChina

coxsackievirus(CV)B belongs to the species Enterovirus B,genus Enterovirus of the family *** B(EV-B)includes 63 serotypes:CVB1-6;CVA9;echoviruses E1-7,9,11-21,24-27,and 29-33;EV-B69,EV-B 73-75,EV-B77-88,EV-B 93,EV-B 9... 详细信息

关键词： coxsackievirus Patients belongs

Identifying potential compounds from Bacopa monnieri(brahmi)against coxsackievirus A16 RdRp targeting HFM disease(tomato flu)

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Medicine in Novel Technology and Devices 2023年第4期20卷 87-104页

作者： Parveen Punia Arun Prajapati Priyasha Maitra Avinash Mishra Pt.Neki Ram Sharma Government College RohtakHaryana124001India Growdea Technologies Pvt.Ltd. Gurugram.Haryana122004India

Hand,foot,and mouth disease(HFMD),primarily instigated by coxsackievirus A16(CVA16),poses a serious health concern,necessitating effective therapeutic *** RNA-dependent RNA polymerase(RdRp)of CVA16 emerges as a promising drug target for HFMD *** study presents an in-silico pipeline for the identification of potential RdRp inhibitors against CVA16.A library of 91 natural compounds derived from Bacopa monnieri(brahmi)was virtually screened against the CVA16 ***,Bacobitacin D emerged as a promising hit molecule,forming 8 hydrogen bonds including key catalytic site residues(Asp^(238)and Asp^(329))within the RdRp active ***,molecular dynamics(MD)simulations and MM/GBSA binding free energy calculations was applied on the top three hits that were selected based on exhaustive docking scores(≤-9.55 kcal/mol).Bacobitacin D exhibited sustainable stability,as evidenced by minimal deviation(RMSD=0.75±0.02 nm)during a 100 ns MD ***,Bacopaside IV exhibited the lowestΔGTOTAL binding free energy(-23.70 kcal/mol),while Bacobitacin D displayed a comparableΔGTOTAL of19.14 kcal/*** interpretation of the most populated cluster derived from MD simulations showed direct interactions of Bacobitacin D with pivotal catalytic residues,including Asp^(238)and Ser^(289).This comprehensive study confirmed Bacobitacin D as a potent inhibitor of CVA16 RdRp,offering a potential avenue for therapeutic intervention against *** validation is required to confirm the inhibitory action of Bacobitacin D against HFMD.

关键词： RNA-Dependent RNA polymerase Hand Foot And mouth disease coxsackievirus Molecular dynamics simulation Molecular mechanics with generalized born andsurface area solvation binding free energy Virtual screening

coxsackievirus B_(3m)与T-2毒素对小鼠脂质过氧化物代谢的影响

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中国地方病学杂志 1999年第3期18卷 173-175页

作者：曲波尹新华刘立志于维汉哈尔滨医科大学克山病研究所

目的探讨Ｔ－２毒素与ＣｏｘｓａｃｋｉｅｖｉｒｕｓＢ３ｍ的双重作用对小鼠肝脏脂质过氧化物的影响。方法实验采用析因设计，ＢＡＬＢ／Ｃ小鼠随机分成４组，Ｉ正常对照组；Ⅱ病毒组；ⅢＴ－２毒素组；Ⅳ病毒＋毒素组。病毒组常规腹腔... 详细信息

目的探讨Ｔ－２毒素与ＣｏｘｓａｃｋｉｅｖｉｒｕｓＢ３ｍ的双重作用对小鼠肝脏脂质过氧化物的影响。方法实验采用析因设计，ＢＡＬＢ／Ｃ小鼠随机分成４组，Ｉ正常对照组；Ⅱ病毒组；ⅢＴ－２毒素组；Ⅳ病毒＋毒素组。病毒组常规腹腔接种０．１ｍｌ内含１０００ＴＣＩＤ５０的１６４０营养液；毒素组隔日按１．０ｍｇ／ｋｇ体重Ｔ－２毒素灌胃，直至实验结束；病毒＋毒素组先Ｔ－２灌胃，３周后腹腔接种同稀释度病毒。各组鼠于病毒接种后第７天，１４天，２１天，３０天，５０天分批处死测定肝脏脂质过氧化物（ＬＰＯ）含量。结果显示病毒和Ｔ－２毒素均可非常明显（Ｐ＜０．０１）引起小鼠肝脏ＬＰＯ含量升高。两者双重作用更为明显，但两者之间没有交互作用。结论ＣｏｘｓａｃｋｉｅｖｉｒｕｓＢ３ｍ和Ｔ－２毒素作用于生物体可导致机体脂质过氧化损伤。

关键词： T-2毒素 coxsackievirus 脂质过氧化物克山病

Evaluation of Antibodies Induced by the Injection of Single Capsid Protein or Purified Virus Particle of coxsackievirus B3 in Mice

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World Journal of Vaccines 2014年第4期4卷 164-174页

作者： Toshiaki Shimoyama Takumi Kubota Junsuke Shirai Rie Watanabe Laboratory of Epizootiology Cooperative Department of Veterinary Medicine Faculty of Agriculture Tokyo University of Agriculture and Technology Fuchu Japan Research and Education Center for Prevention of Global Infectious Disease of Animals Fuchu Japan Veterinary Hygiene Joint Faculty of Veterinary Medicine Yamaguchi University Yamaguchi Japan

Four capsid proteins (VP1, VP2, VP3, and VP4) of coxsackievirus B3 (CVB3) were expressed as recombinant proteins in an Escherichia coli expression system and used as antigens for subunit vaccines against CVB3 in ICR mice. Antigens were expressed as thioredoxin-histidine (TrxHis)-tagged protein and purified before immunization. Although all VPs other than VP4 induced anti-CVB3 specific antibodies in mice (detected by ELISA and western blotting), they did not neutralize the infectious CVB3 in a virus neutralization assay. Meanwhile, 2 virus strains were purified from CVB3 virus stock on the basis of their plaque size on HeLa cells. ICR mice were infected with the 2 purified virus strains (S-strain and L-strain) and unpurified virus stock (wild type) to analyze the difference in antibody responses against infections of purified and unpurified virus strains. The reactivity of antisera against each virus strain was tested by ELISA, and the results showed that the inoculation of purified virus strain induced a strong antibody response against the inoculated strain. As a result, the antibody response against wild-type and other virus strains was suppressed. These results suggest using unpurified virus stock as an antigen is advantageous for inducing a broad antibody response in inoculated animals.

关键词： coxsackievirus B3 Antibody Response Cross Reactivity

Evaluation of Reverse Transcription Loop-Mediated Isothermal Amplification assays for Rapid Detection of Human Enterovirus 71 and coxsackievirus A16 in Clinical Samples

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Advances in Infectious Diseases 2012年第4期2卷 110-118页

作者： Hong Zhang Kai Nie Yunzhi Liu Le Luo Wei Huang Shuaifeng Zhou Mengjie Yang Yu Chen Jianmin Luo Lidong Gao Xuejun Ma Department of Microbiology Center for Disease Control and Prevention of HuNan Changsha China State Key Laboratory for Molecular Virology and Genetic Engineering National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China

A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for human enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) infection was further evaluated. The one step reaction was performed in a single tube at 65?C for 45 min for EV71 and 35 min for CVA16. The detection limits of RT-LAMP assays for both EV71 and CVA16 were 0.1 of a 50% tissue culture infective dose (TCID50) per reaction, based on 10—Fold dilutions of a titrated EV71 or CVA16 strain. The specific assay showed there were no cross-reactions with coxsackievirus A (CVA) viruses (CVA 2, 4, 5, 7, 9, 10, 14, and 25), coxsackievirus B (CVB) viruses (CVB 1, 2, 3, 4, and 5) or ECHO viruses (ECHO 3, 6, 11, and 19). In parallel with commercial quantitative real-time polymerase chain reaction (qRT-PCR) diagnostic kits for EV71 and CVA16, the RT-LAMP assay was evaluated with 515 clinical specimens, the results showed the RT-LAMP assay and the qRT-PCR assay were in complete agreement for 513/515 (99.6%) of the specimens. Two samples with discrepant results from two methods were further verified by nested reverse transcription polymerase chain reaction (nRT-PCR) assay and sequencing to be true positives for CVA16. In conclusion, RT-LAMP assay is demonstrated to be a sensitive and specific assay and have a great potential for the rapid and visual screening of EV71 and CVA16 in China, especially in those resource-limited hospitals and rural clinics of provincial and municipal regions.

关键词： Human Enterovirus 71 coxsackievirus A16 Reverse Transcription Loop-Mediated Isothermal Amplification