检索结果-南通市图书馆

Improving Cross-Protection against Influenza Virus Using Recombinant Vaccinia Vaccine Expressing NP and M2 Ectodomain Tandem Repeats

引用

Virologica Sinica 2019年第5期34卷 583-591页

作者： Wenling Wang Baoying Huang Xiuping Wang Wenjie Tan Li Ruan NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

Conventional influenza vaccines need to be designed and manufactured ***,they occasionally provide poor protection owing to antigenic ***,there is an urgent need to develop universal vaccines against influenza *** nucleoprotein(NP)and extracellular domain of matrix protein 2(M2e)genes from the influenza A virus A/Beijing/30/95(H3N2),we constructed four recombinant vaccinia virus-based influenza vaccines carrying NP fused with one or four copies of M2e genes in different *** recombinant vaccinia viruses were used to immunize BALB/C *** and cellular responses were measured,and then the immunized mice were challenged with the influenza A virus A/Puerto Rico/8/34(PR8).NP-specific humoral response was elicited in mice immunized with recombinant vaccinia viruses carrying full-length NP,while robust M2e-specific humoral response was elicited only in the mice immunized with recombinant vaccinia viruses carrying multiple copies of *** recombinant viruses elicited NP-and M2e-specific cellular immune responses in *** immunization with RVJ-4M2eNP induced remarkably higher levels of IL-2 and IL-10 cytokines specific to ***,RVJ-4M2eNP immunization provided the highest cross-protection in mice challenged with 20 MLD5〇of ***,the cross-protection potentially correlates with both NP and M2e-specific humoral and cellular immune responses induced by RVJ-4M2eNP,which expresses a fusion antigen of full-length NP preceded by four M2e *** results suggest that the rational fusion of NP and multiple M2e antigens is critical toward inducing protective immune responses,and the 4M2eNP fusion antigen may be employed to develop a universal influenza vaccine.

关键词： Influenza A virus(IAV) Cross-protection Recombinant vaccinia virus conserved antigen

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Lactobacillus plantarum displaying conserved M2e and HA2 fusion antigens induces protection against influenza virus challenge

Lactobacillus plantarum displaying conserved M2e and HA2 fus...

引用

中国畜牧兽医学会动物微生态学分会第五届第十三次全国学术研讨会

作者： Wen-Tao Yang Gui-Lian Yang Liang Zhao Yu-Bei Jin Yan-Long Jiang Hai-Bin Huang Chun-Wei Shi Jian-Zhong Wang Guan Wang Yuan-Huan Kang Gui-Lian Yang Chun-Feng Wang College of Animal Science and Technology Jilin Provincial Engineering Research Center of Animal ProbioticsKey Laboratory of Animal Production and Product Quality Safety of Ministry of EducationJilin Agricultural University

Avian influenza virus(AIV) can infect poultry,mammals and other hosts and causes enormous economic losses to the global poultry *** this study,to develop a novel and potent oral vaccine based on Lactobacillus plantarum(***) for controlling the spread of AIV in the poultry industry,we constructed a recombinant *** strain displaying the 3 M2 e-HA2 protein of the influenza virus and determined the effect of N/pgsA’-3 M2 e-HA2 against ATV in *** first confirmed that the 3 M2 e-HA2 fusion protein was expressed on the surface of *** via flow cytometry and immunofluorescence *** experimental results demonstrated that chicks immunized with N/pgsA’-3 M2 e-HA2 could induce specific humoral,mucosal and T cell-mediated immune responses,eliciting the host body to protect itself against ***,compared to oral administration,the intranasal immunization of chicks with N/pgsA’-3 M2 e-HA2 provided a stronger immune response,resulting in a potent protective effect that hindered the loss of body weight,decreasing pulmonary virus titers and reducing lung and throat pathological ***,our results indicate that our novel approach is an effective method of vaccine design to promote mucosal immunity.

关键词： conserved antigen Lactobacillus plantarum influenza virus mucosal immunity

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：